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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||17 Antibodies||36 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies||10 Antibodies|
|Immunofluorescence (IF)||7 Antibodies||7 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||26 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||13 Antibodies|
|Immunoprecipitation (IP)||13 Antibodies|
|Simple Western (SimWes)||1 Antibodies|
|Western Blotting (WB)||54 Antibodies|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Flow Cytometry (FACS)||19 Antibodies|
|Antigen||Annexin A5 (ANXA5) Antibodies|
|Epitope||AA 2-320 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Annexin V antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
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Product Details anti-Annexin V AntibodyTarget Details Annexin V Application Details Handling ProductDetails: References for anti-Annexin V antibody (ABIN3043502) Images
|Purpose||Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: annexin A5
Protein Name: Annexin A5
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human Annexin V recombinant protein (Position: A2-D320). Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.|
Target Details Annexin VProduct Details anti-Annexin V Antibody Application Details Handling ProductDetails: References for anti-Annexin V antibody (ABIN3043502) Images back to top
|Alternative Name||ANXA5 (ANXA5 Antibody Abstract)|
Annexin A5 (or annexin V) is a cellular protein in the annexin group. It is mapped to 4q27. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. And Annexin A5 can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Synonyms: Anchorin CII antibody|Annexin 5 antibody|Annexin A5 antibody|Annexin V antibody|Annexin-5 antibody|Annexin5 antibody|AnnexinA5 antibody|AnnexinV antibody|ANX 5 antibody|ANX A5 antibody|ANX5 antibody|ANXA5 antibody|ANXA5_HUMAN antibody|Calphobindin I antibody|CBP I antibody|CBP-I antibody|Endonexin II antibody|ENX 2 antibody|ENX2 antibody|Lipocortin V antibody|PAP I antibody|PAP-I antibody|Placental anticoagulant protein 4 antibody|Placental anticoagulant protein I antibody|PLACENTAL PROTEIN 4 antibody|PP 4 antibody|PP4 antibody|Thromboplastin inhibitor antibody|VAC alpha antibody|VAC-alpha antibody|Vascular anticoagulant alpha antibody|Vascular anticoagulant-alpha antibody
Application DetailsProduct Details anti-Annexin V Antibody Target Details Annexin V Handling ProductDetails: References for anti-Annexin V antibody (ABIN3043502) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for Annexin V is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Annexin V Antibody Target Details Annexin V Application Details ProductDetails: References for anti-Annexin V antibody (ABIN3043502) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ProductDetails: References for anti-Annexin V antibody (ABIN3043502)Product Details anti-Annexin V Antibody Target Details Annexin V Application Details Handling Images back to top
|Product cited in:||
Sun, Kang, Yao, Chen, Sun, An, Jiang, Wang, Hu: "Dehydrocostus lactone suppressed the proliferation, migration, and invasion of colorectal carcinoma through the downregulation of eIF4E expression." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 641-8, 2015
Ji, Li, Wei, Kong, Jin, Chen, Li, Deng: "Effect of celastrol on growth inhibition of prostate cancer cells through the regulation of hERG channel in vitro." in: BioMed research international, Vol. 2015, pp. 308475, 2015
Hu, Dong, Shi, Deng, Li, Lu: "Evaluation of a novel tetra-functional branched poly(ethylene glycol) crosslinker for manufacture of crosslinked, decellularized, porcine aortic valve leaflets." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 102, Issue 2, pp. 322-36, 2014
Xiong, Ma, Zhang, Shao, Zhang, Zhou: "Apoptosis induced by ?,?-dimethylacrylshikonin is associated with Bcl-2 and NF-?B in human breast carcinoma MCF-7 cells." in: Oncology letters, Vol. 6, Issue 6, pp. 1789-1793, 2013
Jin, Liu, Wang, Xu, Yang, Lu, Sun, Hu: "Protective effects of [Gly14]-Humanin on beta-amyloid-induced PC12 cell death by preventing mitochondrial dysfunction." in: Neurochemistry international, Vol. 56, Issue 3, pp. 417-23, 2010
ImagesProduct Details anti-Annexin V Antibody Target Details Annexin V Application Details Handling ProductDetails: References for anti-Annexin V antibody (ABIN3043502) back to top