anti-Zebrafish (Danio rerio) Annexin V antibody for Immunocytochemistry

Recommended Annexin V Antibody (supplied by: Log in to see )

Antigen
Annexin A5 (ANXA5) Antibodies
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
  • annexin A5
  • annexin A5b
  • Annexin A5
  • annexin 5
  • annexin A5-like
  • ANXA5
  • anxa5b
  • anxa5
  • LOC100220003
  • LOC100305020
  • LOC100342904
  • Anxa5
  • LOC100521982
Reactivity
Human, Zebrafish (Danio rerio)
231
80
58
15
8
7
7
7
6
4
3
2
2
2
2
1
Host
Mouse
165
94
1
Clonality
Monoclonal
Conjugate
This Annexin V antibody is un-conjugated
26
24
8
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
206
143
103
68
41
29
29
27
8
7
6
2
2
1
1
1
1
Supplier
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Catalog No. ABIN5539965
$ 392.70
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN920518 ICC IHC (fro) IF IHC (p) ELISA Mouse IgG1 Log in to see RUU-WAC2A 1

General

Antigen Annexin A5 (ANXA5) Antibodies
Reactivity Human, Zebrafish (Danio rerio)
(231), (80), (58), (15), (8), (7), (7), (7), (6), (4), (3), (2), (2), (2), (2), (1)
Host Mouse
(165), (94), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Annexin V antibody is un-conjugated
(26), (24), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(206), (143), (103), (68), (41), (29), (29), (27), (8), (7), (6), (2), (2), (1), (1), (1), (1)
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Product Details anti-Annexin V Antibody

Target Details Annexin V Application Details Handling Images
Specificity RUU-WAC2A is a monoclonal that specifically reacts with Annexin A5. This monoclonal antibody is directed to the phospholipid binding domain of Annexin A5 and as such it inhibits Annexin A5 binding to anionic phospholipid membranes for more than 95 % . does not remove Annexin A5 bound to phospholipid surfaces indicating that the epitope is not accessible after interaction with Annexin A5. reacts against human recombinant and endogenous annexin A5. It shows no cross reactivity with Annexins I, II, III, IV, VI, VII and VIII in ELISA or in immunoprecipitation. It does not recognise Monkey and Rat Annexin V in ELISA.
Purification Purified
Immunogen Recombinant Annexin A5.
Clone RUU-WAC2A
Isotype IgG1

Target Details Annexin V

Product Details anti-Annexin V Antibody Application Details Handling Images back to top
Antigen
Alternative Name annexin a5,anxa5 (ANXA5 Antibody Abstract)
Background Annexin A5 (or annexin V) is a cellular protein of the annexin family. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin, and also to inhibit the activity of phospholipase A1. Annexin A5 forms a shield around negatively-charged phospholipid molecules, which blocks the entry of phospholipids into coagulation (clotting) reactions. Antibodies directed against annexin A5 are the cause of the antiphospholipid syndrom in which the formation of the shield is disrupted by antibodies. Without the shield, there is an increased quantity of phospholipid molecules on cell membranes, speeding up coagulation reactions and causing the blood-clotting characteristic of the antiphospholipid antibody syndrome. Annexin A5 is used as a probe in the annexin A5 affinity assay to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis aswell as other forms of cell death (1,2). Platelets also expose phosphatidyl-serine on their surface when activated, which serves as binding site for various coagulation factors. Annexin A5 has been shown to interact with kinase insert domain receptor (3) and integrin beta 5 (4).
UniProt P08758
Pathways Apoptosis

Application Details

Product Details anti-Annexin V Antibody Target Details Annexin V Handling Images back to top
Application Notes ELISA in combination with a polyclonal antibody raised against Annexin V in Rabbit. Immunofluorescence to parafolmaldehyde fixed cell samples. Immunohistochemistry on Frozen, Immunohistochemistry on Formaldehyde Fixed, Paraffin-Embedded Sections. For Immunohistochemistry of paraffin sections a pretreatment step with 2 % amino-propyl-triethoxysilan (in 100 % ethanol) and 0,2 % BSA is recommended. The sections are heated for 15 minutes at 100 °C in a citrate buffer (10 mM trisodium citrate, pH 6,0). The sections are then further processed by routine procedures. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/500 for immunoblotting applications.
Restrictions For Research Use only

Handling

Product Details anti-Annexin V Antibody Target Details Annexin V Application Details Images back to top
Format Liquid
Buffer Buffer System: PBS Preservatives: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.