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|Antigen||Annexin A5 (ANXA5) ELISA Kits|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
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Product Details Annexin V ELISA KitTarget details Application Details Handling Images
ELISA kit for the detection of Annexin V in the research laboratory
Alternative Names: Annexin V ELISA kit
Target detailsProduct Details Annexin V ELISA Kit Application Details Handling Images back to top
|Alternative Name||Annexin V (ANXA5 ELISA Kit Abstract)|
Annexins are a family of calcium-dependent phospholipid-binding proteins. They are abundant in the eukaryotic kingdom. Though structurally well investigated the in vivo function of the annexins is still unclear. They definitively belong to a family of ubiquitous cytoplasmic proteins involved in signal transduction. All annexins have been shown to have a putative binding site for protein kinases C but only annexin V possesse a potential pseudo-substrate site. Thus annexin V seems to modulate the activity of some PKCs on their substrates. Annexin V was found to play a major role in matrix vesicleinitiated cartilage calcification as a collageregulated calcium channel. Annexin V binds to procoagulant phospholipids (Vascular anticoagulant alpha) with high affinity. Annexin V's preferential binding partner is phosphatidylserine (PS). PS is predominantly located in membrane leaflets, which face the cytosol.
Synonyms: Annexin V ELISA kit.
|Research Area||Cardiovascular, Atherosclerosis, Apoptosis/Necrosis, Coagulation|
Application DetailsProduct Details Annexin V ELISA Kit Target details Handling Images back to top
|Application Notes||Optimal conditions to be determined by end-user|
|Assay Procedure||An antihuman Annexin V coating antibody is adsorbed onto microwells. Human Annexin V present in the sample orstandard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman Annexin V antibody is added and binds to human Annexin V captured by the first antibody. Following incubation unbound biotinconjugated antihuman Annexin V antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antihuman Annexin V antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human Annexin V present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human Annexin V standard dilutions and human Annexin V sample concentration determined.|
|Restrictions||For Research Use only|
HandlingProduct Details Annexin V ELISA Kit Target details Application Details Images back to top
|Storage Comment||Store at 2-8 °C.|