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Chemical Annexin V ELISA Kit

Recommended Annexin V Product (supplied by: Log in to see )

Antigen
Annexin A5 (ANXA5) ELISA Kits
  • anx
  • Anx5
  • ANX5
  • anx5
  • anxa5
  • ANX V
  • cb989
  • ENX2
  • enx2
  • LC5
  • MGC89158
  • PP4
  • R74653
  • RPRGL3
  • wu:fa98f06
  • wu:fj10f10
Reactivity
Chemical
Alternatives
Kits with alternative reactivity to:
19
18
12
12
8
7
7
6
5
5
4
3
3
2
2
1
1
Application
ELISA
Supplier
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Catalog No. ABIN2869686
$ 1,295.25
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012117105 ABIN2869847 Indirect ELISA Log in to see

Similar Annexin V ELISA Kits

Application / Reactivity Chemical
ELISA 2 ELISA Kits

General

Antigen Annexin A5 (ANXA5) ELISA Kits
Reactivity Chemical
Kits with alternative reactivity to:
(19), (18), (12), (12), (8), (7), (7), (6), (5), (5), (4), (3), (3), (2), (2), (1), (1)
Application ELISA
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Product Details Annexin V ELISA Kit

Target details Application Details Handling Images
Detection Method Colorimetric
Characteristics ELISA kit for the detection of Annexin V in the research laboratory
Alternative Names: Annexin V ELISA kit

Target details

Product Details Annexin V ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Annexin V (ANXA5 ELISA Kit Abstract)
Target Type Chemical
Background Annexins are a family of calcium-dependent phospholipid-binding proteins. They are abundant in the eukaryotic kingdom. Though structurally well investigated the in vivo function of the annexins is still unclear. They definitively belong to a family of ubiquitous cytoplasmic proteins involved in signal transduction. All annexins have been shown to have a putative binding site for protein kinases C but only annexin V possesse a potential pseudo-substrate site. Thus annexin V seems to modulate the activity of some PKCs on their substrates. Annexin V was found to play a major role in matrix vesicleinitiated cartilage calcification as a collageregulated calcium channel. Annexin V binds to procoagulant phospholipids (Vascular anticoagulant alpha) with high affinity. Annexin V's preferential binding partner is phosphatidylserine (PS). PS is predominantly located in membrane leaflets, which face the cytosol.
Synonyms: Annexin V ELISA kit.
Research Area Cardiovascular, Atherosclerosis, Apoptosis/Necrosis, Coagulation
Pathways Apoptosis

Application Details

Product Details Annexin V ELISA Kit Target details Handling Images back to top
Application Notes Optimal conditions to be determined by end-user
Assay Procedure An antihuman Annexin V coating antibody is adsorbed onto microwells. Human Annexin V present in the sample orstandard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman Annexin V antibody is added and binds to human Annexin V captured by the first antibody. Following incubation unbound biotinconjugated antihuman Annexin V antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antihuman Annexin V antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human Annexin V present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human Annexin V standard dilutions and human Annexin V sample concentration determined.
Restrictions For Research Use only

Handling

Product Details Annexin V ELISA Kit Target details Application Details Images back to top
Storage 4 °C
Storage Comment Store at 2-8 °C.