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anti-Human BAK1 antibody for Western Blotting

Recommended BAK1 Antibody (supplied by: Log in to see )

Antigen
BCL2-Antagonist/killer 1 (BAK1) Antibodies
  • ATBAK1
  • ATSERK3
  • BRI1-associated receptor kinase
  • ELG
  • ELONGATED
  • F17M5.190
  • F17M5_190
  • RECEPTOR KINASES LIKE SERK 10
  • RKS10
  • SERK3
  • SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3
  • Bak1
  • BAK
  • BAK-LIKE
  • BCL2L7
  • CDN1
  • Bak
  • N-BAK1
  • N-Bak
  • Leu-rich receptor Serine/threonine protein kinase BAK1
  • Bak protein
  • BCL2-antagonist/killer 1
  • BCL2-antagonist/killer 1-like
  • BAK1
  • BAK
  • Bak1
  • LOC100342046
Epitope
AA 56-91, BH3 Domain
71
20
15
10
5
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
183
87
39
6
3
3
1
1
Host
Rabbit
135
28
21
1
Clonality
Polyclonal
Conjugate
This BAK1 antibody is un-conjugated
5
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
152
68
59
32
29
28
14
13
4
4
3
2
1
Options
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Catalog No. ABIN388093
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0741942 ABIN1533215 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
3.0741942 ABIN492384 EIA IF WB Mouse IgG2a AA 29-187 Log in to see AT8B4 3
3.0741942 ABIN184699 ELISA WB Goat N-Term Log in to see Polyclonal 1
3.0741942 ABIN967519 WB Mouse IgG1 Log in to see G317-2 4
3.0741942 ABIN499405 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 4
3.0741942 ABIN257856 ELISA WB Goat N-Term Log in to see Polyclonal 1
3.0741942 ABIN674409 IF (p) IHC (p) WB Rabbit IgG AA 20-65 Log in to see Polyclonal 2
3.0741942 ABIN3183478 ELISA IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal
3.0741942 ABIN4282996 IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
3.0741942 ABIN2857046 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
3.0741942 ABIN3042342 IHC (p) WB Rabbit IgG AA 22-211 Log in to see Polyclonal
3.0741942 ABIN3044400 ICC IHC (p) WB Rabbit IgG AA 74-95, Middle Region Log in to see Polyclonal
3.0741942 ABIN2801911 WB Rabbit IgG Log in to see Polyclonal 1
3.0741942 ABIN615464 IHC (p) WB Rabbit AA 1-14, N-Term Log in to see Polyclonal
3.0741942 ABIN2707353 IHC WB Rabbit N-Term Log in to see Polyclonal
3.0741942 ABIN2779335 WB Rabbit N-Term Log in to see Polyclonal
3.0741942 ABIN1871224 ICC IHC WB Rabbit IgG Log in to see Polyclonal
3.0741942 ABIN2465179 ELISA WB Goat N-Term Log in to see Polyclonal
3.0741942 ABIN450021 IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
3.0741942 ABIN3020677 WB Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-BAK1 antibody for Western Blotting

Similar anti-BAK1 Antibodies

Application / Reactivity Human
ELISA 68 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 29 Antibodies
Immunofluorescence (IF) 30 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 60 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Western Blotting (WB) 153 Antibodies

General

Antigen BCL2-Antagonist/killer 1 (BAK1) Antibodies
Epitope AA 56-91, BH3 Domain
(71), (20), (15), (10), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(183), (87), (39), (6), (3), (3), (1), (1)
Host Rabbit
(135), (28), (21), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BAK1 antibody is un-conjugated
(5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(152), (68), (59), (32), (29), (28), (14), (13), (4), (4), (3), (2), (1)
Pubmed 2 references available
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Product Details anti-BAK1 Antibody

Target Details BAK1 Application Details Handling References for anti-BAK1 Antibody (ABIN388093) Images
Specificity This Bak antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66~96 amino acids from the N-terminal region (BH3 domain) of human Bak.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This Bak antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-91 AA from human Bak.
Clone RB04682
Isotype Ig

Target Details BAK1

Product Details anti-BAK1 Antibody Application Details Handling References for anti-BAK1 Antibody (ABIN388093) Images back to top
Antigen
Alternative Name Bak (BAK1 Antibody Abstract)
Background BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
Synonyms: BCL2-antagonist/killer 1, cell death inhibitor 1, apoptosis regulator BAK, Bcl-2 homologous antagonist/killer, BCL2-like 7protein
Molecular Weight 23411 DA
Gene ID 578
UniProt Q16611
Research Area Apoptosis/Necrosis, Cell Structure, Cancer
Pathways Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BAK1 Antibody Target Details BAK1 Handling References for anti-BAK1 Antibody (ABIN388093) Images back to top
Application Notes IHC (p) = 1:50-100, WB = 1:1000
Restrictions For Research Use only

Handling

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details References for anti-BAK1 Antibody (ABIN388093) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BAK1 Antibody (ABIN388093)

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling Images back to top
Background publications

Werner, de Vries, Tait, Bontjer, Borst: "Bcl-2 family member Bfl-1/A1 sequesters truncated bid to inhibit is collaboration with pro-apoptotic Bak or Bax." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22781-8, 2002

Bellosillo, Villamor, López-Guillermo, Marcé, Bosch, Campo, Montserrat, Colomer: "Spontaneous and drug-induced apoptosis is mediated by conformational changes of Bax and Bak in B-cell chronic lymphocytic leukemia." in: Blood, Vol. 100, Issue 5, pp. 1810-6, 2002

Images

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling References for anti-BAK1 Antibody (ABIN388093) back to top
Supplier Images
Western Blotting (WB) image for anti-BAK1 antibody (BCL2-Antagonist/killer 1) (AA 56-91) (ABIN388093) Bak-BH3 (ABIN388093) western blot analysis in 293 cell line lysates (35 µg/lane).This...
Immunohistochemistry (IHC) image for anti-BAK1 antibody (BCL2-Antagonist/killer 1) (AA 56-91) (ABIN388093) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...