anti-Rat (Rattus) BAK1 antibody for Enzyme Immunoassay

Recommended BAK1 Antibody (supplied by: Log in to see )

Antigen
BCL2-Antagonist/killer 1 (BAK1) Antibodies
  • ATBAK1
  • ATSERK3
  • BRI1-associated receptor kinase
  • ELG
  • ELONGATED
  • F17M5.190
  • F17M5_190
  • RECEPTOR KINASES LIKE SERK 10
  • RKS10
  • SERK3
  • SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3
  • Bak1
  • BAK
  • BAK-LIKE
  • BCL2L7
  • CDN1
  • Bak
  • N-BAK1
  • N-Bak
  • Leu-rich receptor Serine/threonine protein kinase BAK1
  • Bak protein
  • BCL2-antagonist/killer 1
  • BCL2-antagonist/killer 1-like
  • BAK1
  • BAK
  • Bak1
  • LOC100342046
Epitope
N-Term
71
21
15
11
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
188
87
38
6
3
2
1
1
1
Host
Goat
141
28
20
1
Clonality
Polyclonal
Conjugate
This BAK1 antibody is un-conjugated
5
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
159
72
62
35
32
32
14
13
5
3
3
2
1
Supplier
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Catalog No. ABIN5552724
$ 375.38
Plus shipping costs $45.00

General

Antigen BCL2-Antagonist/killer 1 (BAK1) Antibodies
Epitope N-Term
(71), (21), (15), (11), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(188), (87), (38), (6), (3), (2), (1), (1), (1)
Host Goat
(141), (28), (20), (1)
Clonality
Polyclonal
Conjugate This BAK1 antibody is un-conjugated
(5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(159), (72), (62), (35), (32), (32), (14), (13), (5), (3), (3), (2), (1)
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Product Details anti-BAK1 Antibody

Target Details BAK1 Application Details Handling Images
Sequence ASGQGPGPPR QE-C
Specificity This antibody detects Bcl-2-like 7 at N-term.
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Immunogen Peptide from N Terminus of the protein sequence according to NP_001179.1 Genename: BAK1

Target Details BAK1

Product Details anti-BAK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2-Like 7 (BAK1 Antibody Abstract)
Background Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.Synonyms: Apoptosis regulator BAK, BAK, BAK1, BCL2L7, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, Bcl2-L-7, CDN1, cell death inhibitor 1
Gene ID 578
NCBI Accession NP_001179
UniProt Q16611
Pathways Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response

Application Details

Product Details anti-BAK1 Antibody Target Details BAK1 Handling Images back to top
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western Blot: 0.5-1 μg/mL. Approx 25 kDa band seen in A431 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.