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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||7 Antibodies||68 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||4 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||29 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||30 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||10 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||24 Antibodies|
|Flow Cytometry (FACS)||4 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Antigen||BCL2-Antagonist/killer 1 (BAK1) Antibodies|
|Epitope||AA 74-95, Middle Region Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This BAK1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-BAK1 AntibodyTarget Details BAK1 Application Details Handling Images
|Sequence||QVGRQLALIG DDINRRYDSE FQ|
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Bcl-2 homologous antagonist/killer(BAK1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: BCL2-antagonist/killer 1
Protein Name: Bcl-2 homologous antagonist/killer
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human BAK1(74-95aa QVGRQLALIGDDINRRYDSEFQ), different from the related mouse and rat sequences by one amino acid.|
Target Details BAK1Product Details anti-BAK1 Antibody Application Details Handling Images back to top
|Alternative Name||BAK1 (BAK1 Antibody Abstract)|
|Background||BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene.The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons.By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6.This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.|
|Pathways||Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-BAK1 Antibody Target Details BAK1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BAK1 Antibody Target Details BAK1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling back to top