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anti-Rat (Rattus) BAK1 antibody for Immunocytochemistry

Recommended BAK1 Antibody (supplied by: Log in to see )

Antigen
BCL2-Antagonist/killer 1 (BAK1) Antibodies
  • ATBAK1
  • ATSERK3
  • BAK
  • Bak
  • BAK-LIKE
  • Bak1
  • BCL2L7
  • BRI1-associated receptor kinase
  • CDN1
  • ELG
  • ELONGATED
  • F17M5.190
  • F17M5_190
  • N-Bak
  • N-BAK1
  • RECEPTOR KINASES LIKE SERK 10
  • RKS10
  • SERK3
  • SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3
Epitope
AA 74-95, Middle Region
76
20
13
11
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
185
73
33
4
4
1
1
1
1
Host
Rabbit
136
28
22
1
Clonality
Polyclonal
Conjugate
This BAK1 antibody is un-conjugated
4
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
157
72
60
31
31
28
13
11
5
3
3
2
Supplier
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Available images

Catalog No. ABIN3044400
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN1871224 ICC IHC WB Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN2403422 ICC IHC WB Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN2988421 ICC IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal
0.0009962841 ABIN3030114 ICC IHC (p) WB Rabbit IgG Middle Region Log in to see Polyclonal

Similar anti-BAK1 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 7 Antibodies 74 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 5 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 30 Antibodies
Immunofluorescence (IF) 1 Antibodies 32 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 9 Antibodies 61 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 22 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies

General

Antigen BCL2-Antagonist/killer 1 (BAK1) Antibodies
Epitope AA 74-95, Middle Region
(76), (20), (13), (11), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(185), (73), (33), (4), (4), (1), (1), (1), (1)
Host Rabbit
(136), (28), (22), (1)
Clonality
Polyclonal
Conjugate This BAK1 antibody is un-conjugated
(4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(157), (72), (60), (31), (31), (28), (13), (11), (5), (3), (3), (2)
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Product Details anti-BAK1 Antibody

Target Details BAK1 Application Details Handling Images
Sequence QVGRQLALIG DDINRRYDSE FQ
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Bcl-2 homologous antagonist/killer(BAK1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: BCL2-antagonist/killer 1
Protein Name: Bcl-2 homologous antagonist/killer
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human BAK1(74-95aa QVGRQLALIGDDINRRYDSEFQ), different from the related mouse and rat sequences by one amino acid.
Isotype IgG

Target Details BAK1

Product Details anti-BAK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name BAK1 (BAK1 Antibody Abstract)
Background BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene.The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons.By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6.This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
UniProt Q16611
Pathways Apoptosis

Application Details

Product Details anti-BAK1 Antibody Target Details BAK1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BAK1 antibody (BCL2-Antagonist/killer 1) (AA 74-95) (ABIN3044400) Anti-BAK antibody, Western blotting All lanes: Anti BAK at 0.5ug/ml Lane 1: HELA Who...
Immunohistochemistry (IHC) image for anti-BAK1 antibody (BCL2-Antagonist/killer 1) (AA 74-95) (ABIN3044400) Anti-BAK antibody, IHC(P) IHC(P): Rat Lung Tissue