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anti-Cat (Feline) BCL2L1 antibody for Western Blotting

Recommended BCL2L1 Antibody (supplied by: Log in to see )

Antigen
BCL2-Like 1 (BCL2L1) Antibodies
  • Bcl(X)L
  • Bcl-X
  • bcl-x
  • bcl-X
  • bcl-x(l)
  • Bcl-xl
  • bcl-xl
  • bcl-XL
  • bcl-xL
  • Bcl-XL
  • BCL-XL
  • Bcl-xL
  • BCL-XL/S
  • bcl-xl/s
  • bcl-xs
  • Bcl-Xs
  • Bcl-xS
  • bcl-xS
  • Bcl2l
  • bcl2l
  • BCL2L
  • BCL2L1
  • BclX
  • Bclx
  • BCLX
  • bclx
  • bclxl
  • BCLXL
  • BCLXS
  • PPP1R52
  • xR11
Epitope
N-Term, AA 30-70
152
34
27
22
19
14
13
13
11
11
11
7
7
7
6
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cat (Feline), Dog (Canine), Human, Sheep (Ovine)
482
305
278
137
16
8
6
5
4
3
2
2
2
1
Host
Rabbit
281
234
4
3
Clonality
Polyclonal
Conjugate
This BCL2L1 antibody is un-conjugated
24
15
10
9
7
7
6
5
5
5
5
5
5
5
5
5
5
5
2
2
2
2
2
2
2
Application
Western Blotting (WB)
405
192
167
157
147
128
125
96
93
23
7
4
3
3
2
1
1
Supplier
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Available images

Catalog No. ABIN259856
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011728023 ABIN4283649 WB DyLight 405 Rabbit AA 30-70, N-Term Log in to see Polyclonal
0.0011728023 ABIN4283648 WB FITC Rabbit AA 30-70, N-Term Log in to see Polyclonal

Similar anti-BCL2L1 Antibodies

Application / Reactivity Human Dog (Canine) Cat (Feline) Sheep (Ovine)
Western Blotting (WB) 388 Antibodies 17 Antibodies 3 Antibodies 5 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Immunoprecipitation (IP) 128 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 184 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 158 Antibodies 6 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 91 Antibodies 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunofluorescence (IF) 150 Antibodies 2 Antibodies

General

Antigen BCL2-Like 1 (BCL2L1) Antibodies
Epitope N-Term, AA 30-70
(152), (34), (27), (22), (19), (14), (13), (13), (11), (11), (11), (7), (7), (7), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cat (Feline), Dog (Canine), Human, Sheep (Ovine)
(482), (305), (278), (137), (16), (8), (6), (5), (4), (3), (2), (2), (2), (1)
Host Rabbit
(281), (234), (4), (3)
Clonality
Polyclonal
Conjugate This BCL2L1 antibody is un-conjugated
(24), (15), (10), (9), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(405), (192), (167), (157), (147), (128), (125), (96), (93), (23), (7), (4), (3), (3), (2), (1), (1)
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Product Details anti-BCL2L1 Antibody

Target Details BCL2L1 Application Details Handling Images
Specificity There are three known isoforms of Bcl-X, Bcl-X(S), Bcl-X(beta), and Bcl-X(L). The immunogen used to create this has 100 % identity to all three isoforms.
Purification Immunogen affinity purified
Immunogen Synthetic peptide made to an N-terminal portion of human BCL2L1 (within residues 30-70). [Swiss-Prot# Q07817]

Target Details BCL2L1

Product Details anti-BCL2L1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-XL (BCL2L1 Antibody Abstract)
Background Gene Symbol: BCL2L1
Molecular Weight Theoretical MW: 26 kDa
Gene ID 598
UniProt Q07817
Pathways Apoptosis

Application Details

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Handling Images back to top
Application Notes Western Blot 2 μg/mL

Not for use in: ICC/IF
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for BCL2L1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 25 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-Bcl-X primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/phosphate, pH 7-8
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

Images

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2L1 antibody (BCL2-Like 1) (N-Term) (ABIN259856) Detection of Bcl-x in A431 whole cell extracts using ABIN259855.