anti-Cat (Feline) BCL2L1 antibody for Western Blotting

Recommended BCL2L1 Antibody (supplied by: Log in to see )

Antigen
BCL2-Like 1 (BCL2L1) Antibodies
  • bcl-XL
  • bcl-x
  • bcl-x(l)
  • bcl-xl/s
  • bcl-xs
  • bcl2l
  • bclx
  • bclxl
  • xR11
  • BCL2L1
  • Bcl-xS
  • BCL-XL/S
  • BCL2L
  • BCLX
  • BCLXL
  • BCLXS
  • Bcl-X
  • PPP1R52
  • bcl-xL
  • bcl-xS
  • Bcl(X)L
  • Bcl-XL
  • Bcl-Xs
  • Bcl2l
  • BclX
  • Bcl-xl
  • Bclx
  • bcl-X
  • BCL-XL
  • Bcl-xL
  • bcl-xl
  • BCL2-like 1
  • transgene insertion 2, Craig B Thompson
  • Bcl2-like 1
  • bcl2l1
  • BCL2L1
  • LOC100224900
  • Tg(BCL2L1)2Cbt
  • Bcl2l1
Epitope
N-Term, AA 1-50
151
36
30
26
19
15
15
12
11
10
10
9
9
7
6
6
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Cat (Feline), Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
476
353
317
150
36
22
17
14
14
13
12
10
4
3
2
2
1
Host
Rabbit
290
221
3
3
Clonality
Polyclonal
Conjugate
This BCL2L1 antibody is un-conjugated
26
17
11
9
7
7
6
6
5
5
5
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Western Blotting (WB)
400
201
170
160
155
149
127
96
93
27
10
8
7
7
3
3
2
2
1
Supplier
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Available images

Catalog No. ABIN265625
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.905818 ABIN259855 WB Rabbit N-Term, AA 30-70 Log in to see Polyclonal
1 ABIN4283648 WB FITC Rabbit AA 30-70, N-Term Log in to see Polyclonal
1 ABIN4283649 WB DyLight 405 Rabbit AA 30-70, N-Term Log in to see Polyclonal

General

Antigen BCL2-Like 1 (BCL2L1) Antibodies
Epitope N-Term, AA 1-50
(151), (36), (30), (26), (19), (15), (15), (12), (11), (10), (10), (9), (9), (7), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Cat (Feline), Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(476), (353), (317), (150), (36), (22), (17), (14), (14), (13), (12), (10), (4), (3), (2), (2), (1)
Host Rabbit
(290), (221), (3), (3)
Clonality
Polyclonal
Conjugate This BCL2L1 antibody is un-conjugated
(26), (17), (11), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Western Blotting (WB)
(400), (201), (170), (160), (155), (149), (127), (96), (93), (27), (10), (8), (7), (7), (3), (3), (2), (2), (1)
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Product Details anti-BCL2L1 Antibody

Target Details BCL2L1 Application Details Handling Images
Specificity There are three known isoforms of Bcl-X, Bcl-X(S), Bcl-X(beta), and Bcl-X(L). The immunogen used to create this has 100 % identity to all three isoforms.
Purification Immunogen affinity purified
Immunogen Synthetic peptide made to an N-terminal portion of human BCL2L1 (within residues 1-50). [Swiss-Prot# Q07817]

Target Details BCL2L1

Product Details anti-BCL2L1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-XL (BCL2L1 Antibody Abstract)
Background Gene Symbol: BCL2L1
Molecular Weight Theoretical MW: 26 kDa
Gene ID 598
UniProt Q07817
Research Area Cancer
Pathways Apoptosis, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Handling Images back to top
Application Notes Western Blot 1:10000This BCL2L1 antibody is useful for Western blot, where a band is seen ~26 kDa. This antibody is not useful for ICC/IF. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Not for use in: ICC/IF
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for BCL2L1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-Bcl-X primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

Images

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2-Like 1 (BCL2L1) (AA 1-50), (N-Term) antibody (ABIN265625) Detection of Bcl-x in A431 whole cell lysate using ABIN265625.
Western Blotting (WB) image for anti-BCL2-Like 1 (BCL2L1) (AA 1-50), (N-Term) antibody (ABIN265625) Western Blot: Bcl-Xl Antibody [ABIN2656259] - Detection of BCL2L1 / Bcl-Xl in whole c...