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anti-Human BCL2L1 antibody for Immunohistochemistry (Frozen Sections)

Recommended BCL2L1 Antibody (supplied by: Log in to see )

Antigen
BCL2-Like 1 (BCL2L1) Antibodies
  • Bcl(X)L
  • Bcl-X
  • bcl-x
  • bcl-X
  • bcl-x(l)
  • Bcl-xl
  • bcl-xl
  • bcl-XL
  • bcl-xL
  • Bcl-XL
  • BCL-XL
  • Bcl-xL
  • BCL-XL/S
  • bcl-xl/s
  • bcl-xs
  • Bcl-Xs
  • Bcl-xS
  • bcl-xS
  • Bcl2l
  • bcl2l
  • BCL2L
  • BCL2L1
  • BclX
  • Bclx
  • BCLX
  • bclx
  • bclxl
  • BCLXL
  • BCLXS
  • PPP1R52
  • xR11
Reactivity
Human
336
170
147
22
10
8
6
5
3
2
2
1
Host
Rabbit
258
112
3
3
Clonality
Polyclonal
Conjugate
This BCL2L1 antibody is un-conjugated
18
13
5
4
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
268
143
91
77
71
51
49
23
21
13
7
4
3
2
2
1
1
Supplier
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Catalog No. ABIN967340
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN371611 FACS IHC (fro) IP WB Mouse IgG3 Log in to see 7B2-5 15
0.00088051456 ABIN957163 IHC (fro) IHC (p) IP WB Rabbit AA 61-79 Log in to see Polyclonal
0.00088051456 ABIN957162 ICC IHC (fro) IHC (p) IP WB Rabbit AA 46-66 Log in to see Polyclonal
0.00088051456 ABIN440455 FACS IHC (fro) IP IHC WB FITC Mouse IgG3 Log in to see 7B2-5
0.00088051456 ABIN440456 FACS IHC (fro) IP IHC WB Biotin Mouse IgG3 Log in to see 7B2-5
0.00088051456 ABIN957161 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00088051456 ABIN1822168 IHC (fro) IHC (p) IP WB Rabbit IgG N-Term Log in to see Polyclonal
0.00088051456 ABIN440454 FACS IHC (fro) IP IHC WB Mouse IgG3 Log in to see 7B2-5
0.00088051456 ABIN1077849 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 2-212 Log in to see Polyclonal
0.00088051456 ABIN184367 IHC (fro) IP WB Rabbit Log in to see Polyclonal
0.00088051456 ABIN187417 EIA IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see 2H12
0.00088051456 ABIN2688850 IHC (f) IHC (fro) WB Rabbit Ig Fraction AA 46-66 Log in to see Polyclonal
0.00088051456 ABIN2590038 IHC (fro) Rabbit IgG Log in to see Polyclonal

Top referenced anti-BCL2L1 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-BCL2L1 Antibodies

Application / Reactivity Human
ELISA 71 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 50 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 70 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 136 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 63 Antibodies
Immunoprecipitation (IP) 51 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Western Blotting (WB) 255 Antibodies

General

Antigen BCL2-Like 1 (BCL2L1) Antibodies
Reactivity Human
(336), (170), (147), (22), (10), (8), (6), (5), (3), (2), (2), (1)
Host Rabbit
(258), (112), (3), (3)
Clonality
Polyclonal
Conjugate This BCL2L1 antibody is un-conjugated
(18), (13), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(268), (143), (91), (77), (71), (51), (49), (23), (21), (13), (7), (4), (3), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-BCL2L1 Antibody

Target Details BCL2L1 Application Details Handling References for anti-BCL2L1 Antibody (ABIN967340) Images
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. This product contains thimerosal, an organic mercury compound. Mercury and mercury compounds are chemicals known to the State of California to cause birth defects or other reproductive harm. Foreseeable use of this product does not pose a known reproductive toxicity threat.
Purification Purified from antiserum by negative adsorption and affinity chromatography.
Components 1) 51-9000093: Rabbit Anti-Serum-Bcl-X.
Quantity: 0.1 ml (1 ea).
Clone: Polyclonal.
Immunogen: Human Bcl-X aa. 46-66 synthetic peptide.
Isotype: Rabbit Ig.
Storage Buffer: Aqueous buffered solution containing Less or equal than 0.09% sodium azide and Less or equal than 0.03% thimerosal

2) 51-16516N: HeLa Control Lysate.
Quantity: 50 µg (1 ea).
Concentration: 1.0 mg/ml.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol).
Immunogen Human Bcl-X synthetic peptide
Isotype Ig

Target Details BCL2L1

Product Details anti-BCL2L1 Antibody Application Details Handling References for anti-BCL2L1 Antibody (ABIN967340) Images back to top
Antigen
Alternative Name Bcl-x (BCL2L1 Antibody Abstract)
Background Members of the Bcl-2 family play a major role in regulating the response of cells to a wide variety of apoptotic signals. The first member of this multifamily, Bcl-2, was discovered in the mid 1980s through its involvement in t(14,18) chromosomal translocations commonly found in a subset of follicular B-cell lymphomas. Bcl-2 blocks apoptosis, and translocation of Bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B cells deregulates Bcl-2 gene expression, resulting in high levels of Bcl-2 mRNA and protein expression. Deregulation of the Bcl-2 gene, by translocations or other mechanisms, appears to contribute to tumorigenesis by prolonging cell survival rather than by accelerating the rate of cell proliferation. A variety of Bcl-2 homologues have since been described in humans and other mammals, leading to the definition of a multigene family. Like Bcl-2, some Bcl-2 family members block apoptosis, whereas others promote apoptosis and inhibit Bcl-2 activity. Bcl-X is a Bcl-2 homologue that has two isoforms, resulting from alternative splicing. Bcl-X-L (long) is a 241 amino acid protein that is 47% homologous to Bcl-2 on the amino acid level. Bcl-X-S (short) is a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 protein family. bcl-X-L blocks cell death, whereas Bcl-X-S inhibits Bcl-2 and promotes cell death. The antibodies recognize both mouse and human Bcl-X-L (long) and Bcl-X-S (short) proteins. A synthetic peptide corresponding to amino acids 46-66 of human Bcl-X was used as immunogen. This region corresponds to a unique domain that lacks homolog with other known members of the Bcl-2 family.
Research Area Cancer
Pathways Apoptosis

Application Details

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Handling References for anti-BCL2L1 Antibody (ABIN967340) Images back to top
Application Notes Applications include western blot analysis (1:1000 - 1:2000)2-4 and immunohistochemical staining (1:500 - 1:2000) of frozen tissue sections and of formalinfixed or Bouin's-fixed, paraffin-embedded tissue sections. The antiserum should be stored at 4°C. HeLa control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (store lysate at -20°C).
Comment

Related Products: ABIN967390, ABIN968535

Restrictions For Research Use only

Handling

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details References for anti-BCL2L1 Antibody (ABIN967340) Images back to top
Format Liquid
Concentration 1.0 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-BCL2L1 Antibody (ABIN967340)

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Handling Images back to top
Product cited in:

Alexander-Miller, Derby, Sarin, Henkart, Berzofsky: "Supraoptimal peptide-major histocompatibility complex causes a decrease in bc1-2 levels and allows tumor necrosis factor alpha receptor II-mediated apoptosis of cytotoxic T lymphocytes." in: The Journal of experimental medicine, Vol. 188, Issue 8, pp. 1391-9, 1998

Reed, Miyashita, Krajewski, Takayama, Aime-Sempe, Kitada, Sato, Wang, Harigai, Hanada, Krajewska, Kochel, Millan, Kobayashi: "Bcl-2 family proteins and the regulation of programmed cell death in leukemia and lymphoma." in: Cancer treatment and research, Vol. 84, pp. 31-72, 1996

Krajewski, Krajewska, Shabaik, Wang, Irie, Fong, Reed: "Immunohistochemical analysis of in vivo patterns of Bcl-X expression." in: Cancer research, Vol. 54, Issue 21, pp. 5501-7, 1994

Background publications

Ohta, Iwai, Kasahara, Taniguchi, Krajewski, Reed, Miyawaki: "Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues." in: International immunology, Vol. 7, Issue 11, pp. 1817-25, 1996

Images

Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Handling References for anti-BCL2L1 Antibody (ABIN967340) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2L1 antibody (BCL2-Like 1) (ABIN967340) Western blot analysis of Bcl-X. Lysate from HeLa cells was probed with anti-Bcl-X. Th...
 image for anti-BCL2L1 antibody (BCL2-Like 1) (ABIN967340) Paraffin-embedded tissue section of a high grade human astrocytoma stained with polyc...