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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||36 Antibodies||38 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies||3 Antibodies|
|Flow Cytometry (FACS)||15 Antibodies||21 Antibodies|
|Immunochromatography (IC)||3 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies|
|Immunofluorescence (IF)||42 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||25 Antibodies|
|Immunohistochemistry (IHC)||75 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||21 Antibodies|
|Immunoprecipitation (IP)||12 Antibodies|
|Western Blotting (WB)||104 Antibodies|
|Antigen||BCL2-Like 1 (BCL2L1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BCL2L1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|15 references available|
|Supplier||Log in to see|
Product Details anti-BCL2L1 AntibodyTarget Details BCL2L1 Application Details Handling References for anti-BCL2L1 Antibody (ABIN371611) Images
|Specificity||This antibody recognizes Bcl-xL.|
|Characteristics||Synonyms: Bcl2-L-1, BCL2L1, BCL2L, BCLX, Bcl-x, bcl-xL, bcl-xS, Bcl-2-like protein 1|
Target Details BCL2L1Product Details anti-BCL2L1 Antibody Application Details Handling References for anti-BCL2L1 Antibody (ABIN371611) Images back to top
|Alternative Name||Bcl-2-Like 1 (BCL2L1 Antibody Abstract)|
|Background||Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems. (Ref.1) It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment. (Ref.2) Bcl-2 and one of its homologues, Bcl-xL, protect cells from apoptosis (Ref.3,4) while other homologues of Bcl-2 such as Bax, Bad and Bak have been shown to enhance apoptosis. (Ref.5-8) Bcl-xL has been shown to block apoptosis which is induced by a variety of stimuli and, under certain conditions, offers greater protection against apoptosis than Bcl-2. (Ref.9-13) In contrast, Bad and Bax inhibit the protective functions of Bcl-xL and Bcl-2, respectively. Although heterodimerization between Bcl-xL/Bad and Bcl-2/Bax was originally thought to be essential for the differential anti-apoptotic activity of Bcl-xL and Bcl-2. (Ref.5,14) Other results suggest that the formation of heterodimers may not be necessary for this death-repressing activity. (Ref.15,16)Synonyms: BCL2L, BCL2L1, BCLX, Bcl-2-like protein 1, Bcl-x, Bcl2-L-1, bcl-xL, bcl-xS|
Application DetailsProduct Details anti-BCL2L1 Antibody Target Details BCL2L1 Handling References for anti-BCL2L1 Antibody (ABIN371611) Images back to top
Flow Cytometry: < / = 3 μg/10^6 cells. Western Blot: < / = 0.5 μg/mL. Immunohistochemistry on Frozen Sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details References for anti-BCL2L1 Antibody (ABIN371611) Images back to top
|Buffer||100 mM Borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-BCL2L1 Antibody (ABIN371611)Product Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Handling Images back to top
Gottschalk, Boise, Oltvai et al.: "The ability of Bcl-x(L) and Bcl-2 to prevent apoptosis can be differentially regulated." in: Cell death and differentiation, Vol. 3, Issue 1, pp. 113-8, 2006 (PubMed).
Nuñez, Clarke: "The Bcl-2 family of proteins: regulators of cell death and survival." in: Trends in cell biology, Vol. 4, Issue 11, pp. 399-403, 2004 (PubMed).
Cheng, Levine, Boise et al.: "Bax-independent inhibition of apoptosis by Bcl-XL." in: Nature, Vol. 379, Issue 6565, pp. 554-6, 1996 (PubMed).
Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993 (PubMed).
Boise, González-García, Postema et al.: "bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death." in: Cell, Vol. 74, Issue 4, pp. 597-608, 1993 (PubMed).
Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, 1994 (PubMed).
Dole, Jasty, Cooper et al.: "Bcl-xL is expressed in neuroblastoma cells and modulates chemotherapy-induced apoptosis." in: Cancer research, Vol. 55, Issue 12, pp. 2576-82, 1995 (PubMed).
González-García, García, Ding et al.: "bcl-x is expressed in embryonic and postnatal neural tissues and functions to prevent neuronal cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 10, pp. 4304-8, 1995 (PubMed).
Shimizu, Eguchi, Kosaka et al.: "Prevention of hypoxia-induced cell death by Bcl-2 and Bcl-xL." in: Nature, Vol. 374, Issue 6525, pp. 811-3, 1995 (PubMed).
Kiefer, Brauer, Powers et al.: "Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 736-9, 1995 (PubMed).
Chittenden, Harrington, OConnor et al.: "Induction of apoptosis by the Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 733-6, 1995 (PubMed).
Farrow, White, Martinou et al.: "Cloning of a bcl-2 homologue by interaction with adenovirus E1B 19K." in: Nature, Vol. 374, Issue 6524, pp. 731-3, 1995 (PubMed).
Cory: "Regulation of lymphocyte survival by the bcl-2 gene family." in: Annual review of immunology, Vol. 13, pp. 513-43, 1995 (PubMed).
Gottschalk, Boise, Thompson et al.: "Identification of immunosuppressant-induced apoptosis in a murine B-cell line and its prevention by bcl-x but not bcl-2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 15, pp. 7350-4, 1994 (PubMed).
Cohen: "Programmed cell death in the immune system." in: Advances in immunology, Vol. 50, pp. 55-85, 1991 (PubMed).
ImagesProduct Details anti-BCL2L1 Antibody Target Details BCL2L1 Application Details Handling References for anti-BCL2L1 Antibody (ABIN371611) back to top