anti-Human BID antibody for Enzyme Immunoassay

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist-like
  • BH3-interacting domain death agonist-like
  • BID
  • bida
  • bid
  • Bid
  • LOC100355991
  • LOC100730546
Epitope
pSer65
57
37
35
27
15
14
11
10
9
8
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
323
175
75
9
5
3
2
Host
Rabbit
299
95
20
9
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
352
188
127
88
54
41
34
23
13
11
10
6
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN358089
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN356816 EIA IHC (p) WB Rabbit Ig BH3 Domain Log in to see Polyclonal 4
1 ABIN5553068 EIA WB Goat C-Term Log in to see Polyclonal
1 ABIN372917 EIA IHC (p) WB Rabbit IgG Middle Region Log in to see Polyclonal
1 ABIN372916 EIA IHC (fro) IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN336094 EIA WB Mouse IgG1 AA 1-195, N-Term Log in to see 4D3
1 ABIN1105530 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3C5 2
1 ABIN5554611 EIA WB Rabbit IgG Middle Region Log in to see Polyclonal
1 ABIN5554610 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN371640 EIA IP WB Mouse IgG2b Log in to see SB50a 10

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Epitope pSer65
(57), (37), (35), (27), (15), (14), (11), (10), (9), (8), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(323), (175), (75), (9), (5), (3), (2)
Host Rabbit
(299), (95), (20), (9)
Clonality
Polyclonal
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(352), (188), (127), (88), (54), (41), (34), (23), (13), (11), (10), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID antibody (ABIN358089) Images
Specificity This antibody detects BID pSer65.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.
Isotype Ig

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID antibody (ABIN358089) Images back to top
Antigen
Alternative Name BID (BID Antibody Abstract)
Background Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.Synonyms: BH3-interacting domain death agonist, p22 BID
Molecular Weight 26836 Da
Gene ID 637
NCBI Accession NP_001187
UniProt P55957
Research Area Cancer
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID antibody (ABIN358089) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID antibody (ABIN358089) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-BID antibody (ABIN358089)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Background publications

Liu, Durrant, Yang, He, Whitby, Myszka, Lee: "The interaction between tBid and cardiolipin or monolysocardiolipin." in: Biochemical and biophysical research communications, Vol. 330, Issue 3, pp. 865-70, 2005

Broaddus, Dansen, Abayasiriwardana, Wilson, Finch, Swigart, Hunt, Evan: "Bid mediates apoptotic synergy between tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and DNA damage." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12486-93, 2005

Weng, Li, Xu, Shi, Tang: "Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10491-500, 2005

Gong, Choi, Franzin, Zhai, Reed, Marassi: "Conformation of membrane-associated proapoptotic tBid." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28954-60, 2004

García-Sáez, Mingarro, Pérez-Payá, Salgado: "Membrane-insertion fragments of Bcl-xL, Bax, and Bid." in: Biochemistry, Vol. 43, Issue 34, pp. 10930-43, 2004

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID antibody (ABIN358089) back to top
Supplier Images
 image for anti-BH3 Interacting Domain Death Agonist (BID) (pSer65) antibody (ABIN358089) anti-BH3 Interacting Domain Death Agonist (BID) (pSer65) antibody
 image for anti-BH3 Interacting Domain Death Agonist (BID) (pSer65) antibody (ABIN358089) anti-BH3 Interacting Domain Death Agonist (BID) (pSer65) antibody (Image 2)