anti-Human BID antibody for ELISA (Detection)

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist-like
  • BH3-interacting domain death agonist-like
  • BID
  • bida
  • bid
  • Bid
  • LOC100355991
  • LOC100730546
Epitope
AA 1-195
56
37
36
27
15
14
11
10
9
8
5
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
321
174
74
9
5
3
2
Host
Mouse
296
95
20
9
Clonality
Monoclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, ELISA (Detection), Immunoprecipitation (IP), Western Blotting (WB)
348
185
127
85
53
41
33
22
13
11
11
6
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1996891
$ 218.90
Plus shipping costs $45.00

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Epitope AA 1-195
(56), (37), (36), (27), (15), (14), (11), (10), (9), (8), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(321), (174), (74), (9), (5), (3), (2)
Host Mouse
(296), (95), (20), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, ELISA (Detection), Immunoprecipitation (IP), Western Blotting (WB)
(348), (185), (127), (85), (53), (41), (33), (22), (13), (11), (11), (6), (3), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-BID Antibody

Target Details BID Application Details Handling Images
Specificity Human BID
No cross-reactivity in ELISA with
E.coli cell lysate
No Cross-Reactivity Escherichia coli (E. coli)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BID (rh BID, ABIN2002909 P55957-1, Met 1-Asp 195,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human BID protein (ABIN2002909)
Clone 3C4C2
Isotype IgG2a

Target Details BID

Product Details anti-BID Antibody Application Details Handling Images back to top
Antigen
Alternative Name BID (BID Antibody Abstract)
Background BID, BH3-interacting domain death agonist, is a member of the Bcl-2 protein family. BID contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. It is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent, and also offer a new strategy for the treatment of acute renal failure associated with ischemia-reperfusion. BID receives direct inputs from a key regulator of the cell cycle arrest/DNA repair machinery (ATM), and therefore is an excellent candidate to coordinate genotoxic stress responses and apoptotic cell death. BID is a novel pro-apoptosis Bcl-2 family protein that is activated by caspase 8 in response to Fas/TNF-R1 death receptor signals. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. Recent studies further indicate that BID may be more than just a killer molecule. Deletion of BID inhibits carcinogenesis in the liver, although this genetic alteration promotes tumorigenesis in the myeloid cells. This is likely related to the function of BID to promote cell cycle progression into S phase. BID could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint.
UniProt P55957-1
Research Area Cancer
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BID Antibody Target Details BID Handling Images back to top
Application Notes WB: 2-5 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human BID. The detection limit for Human BID is approximately 0.078 ng/well.
ELISA(Det): 0.1-1 μg/mL
IP: 4-6 μg/mg of lysate
Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN1996891) anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody
Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (ABIN1996891) anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody (Image 2)