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anti-Human BID antibody for Flow Cytometry

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • DKFZp469E066
  • FP497
  • fp497
  • si:ch211-238n5.6
  • xbid
Reactivity
Human
304
137
62
4
2
2
1
Host
Mouse
277
89
21
10
Clonality
Monoclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
310
185
117
71
40
34
30
18
13
12
12
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN968979
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3116459 ABIN1996893 FACS IF ELISA WB Mouse IgG1 AA 1-195 Log in to see 10
0.3116459 ABIN1996896 FACS FITC Mouse IgG1 AA 1-195 Log in to see 10
0.3116459 ABIN1996898 FACS PE Mouse IgG1 AA 1-195 Log in to see 10
0.3116459 ABIN1845950 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.0010621916 ABIN189116 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG Log in to see Y8
0.0010621916 ABIN1496918 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 3C5
0.0010621916 ABIN965667 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 3C5 2
0.0010621916 ABIN600516 FACS IP IHC ELISA WB Mouse IgG AA 83-103 Log in to see
0.0010621916 ABIN2666317 FACS Rat IgG1 Log in to see 1H11 5
0.0010621916 ABIN865441 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 3C5
0.0010621916 ABIN2210327 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B66
0.0010621916 ABIN2666318 FACS Rat IgG1 Log in to see 1H11 5
0.0010621916 ABIN4284619 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 3C5

Top referenced anti-BID antibody for Flow Cytometry

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Application / Reactivity Human
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ELISA 159 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 14 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 30 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 108 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 55 Antibodies
Immunoprecipitation (IP) 33 Antibodies
Immunostaining (ISt) 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
SDS-PAGE (SDS) 1 Antibodies
Western Blotting (WB) 258 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Reactivity Human
(304), (137), (62), (4), (2), (2), (1)
Host Mouse
(277), (89), (21), (10)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(310), (185), (117), (71), (40), (34), (30), (18), (13), (12), (12), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID Antibody (ABIN968979) Images
Purification Ascites
Immunogen Purified recombinant fragment of human BID expressed in E. coli.
Clone 3C5
Isotype IgG1

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN968979) Images back to top
Antigen
Alternative Name BID (BID Antibody Abstract)
Background This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.Tissue specificity: Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level)
Molecular Weight 22 kDa
Gene ID 637
HGNC 637
Research Area Cancer
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN968979) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN968979) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-BID Antibody (ABIN968979)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Product cited in:

Wan, Liu, Xing, Chen: "Bid is required in NPe6-PDT-induced apoptosis." in: Photochemistry and photobiology, Vol. 84, Issue 1, pp. 250-7, 2008

Wu, Xing, Chen, Wang: "Bid is not required for Bax translocation during UV-induced apoptosis." in: Cellular signalling, Vol. 19, Issue 12, pp. 2468-78, 2007

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN968979) back to top
Supplier Images
ELISA image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN968979) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN968979) Figure 2: Flow cytometric analysis of Hela cells using BID mouse mAb (green) and nega...
Immunocytochemistry (ICC) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN968979) Figure 3: Immunofluorescence analysis of Hela cells using BID mouse mAb (green) . Blu...
Immunohistochemistry (IHC) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN968979) Figure 4: Immunohistochemical analysis of paraffin-embedded prostate tissues (left) a...
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN968979) Figure 5: Western blot analysis using BID mouse mAb against Hela (1), A431 (2), Jurka...