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anti-Human BID antibody for Immunofluorescence

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • DKFZp469E066
  • FP497
  • fp497
  • si:ch211-238n5.6
  • xbid
Epitope
AA 50-62
43
34
32
17
16
14
13
11
9
8
6
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
274
129
56
2
1
Host
Mouse
268
68
21
10
Clonality
Monoclonal
Conjugate
This BID antibody is un-conjugated
20
18
13
8
7
7
3
3
3
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
280
166
108
63
34
34
25
18
12
11
9
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN968577
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3162927 ABIN1105530 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3C5 2
0.3162927 ABIN2788659 IF WB Rabbit C-Term Log in to see Polyclonal
0.3162927 ABIN513721 IF ELISA WB Mouse IgG2a kappa AA 1-195, full length Log in to see 3E8-1B10
0.0010848765 ABIN968686 IF WB Mouse IgG1 AA 46-168 Log in to see 40-Bid 3
0.0010848765 ABIN189116 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG Log in to see Y8
0.0010848765 ABIN1496918 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 3C5
0.0010848765 ABIN1996893 FACS IF ELISA WB Mouse IgG1 AA 1-195 Log in to see 10
0.0010848765 ABIN965667 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 3C5 2
0.0010848765 ABIN2590266 IF Mouse IgG2a Log in to see 3E9
0.0010848765 ABIN2590267 IF Mouse IgG2a Log in to see 3A10
0.0010848765 ABIN2590268 IF Mouse IgG1 Log in to see 3C8
0.0010848765 ABIN2590262 IF Mouse IgG1 Log in to see 4A1
0.0010848765 ABIN2590269 IF Mouse IgG2b Log in to see 2H9
0.0010848765 ABIN2957879 IC IF IHC WB Rabbit Log in to see Polyclonal
0.0010848765 ABIN865441 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 3C5
0.0010848765 ABIN1977020 IF IHC (p) WB Rabbit IgG AA 60-120 Log in to see Polyclonal
0.0010848765 ABIN1977021 IF IHC (p) Rabbit IgG AA 60-120 Log in to see Polyclonal
0.0010848765 ABIN1845950 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.0010848765 ABIN2671495 IF WB Mouse IgG2b Log in to see OTI2H9
0.0010848765 ABIN2671500 IF IHC WB Mouse IgG2a Log in to see OTI3E9

Top referenced anti-BID antibody for Immunofluorescence

Similar anti-BID Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
ELISA 139 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 13 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 98 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 47 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Immunostaining (ISt) 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
SDS-PAGE (SDS) 1 Antibodies
Western Blotting (WB) 228 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Epitope AA 50-62
(43), (34), (32), (17), (16), (14), (13), (11), (9), (8), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(274), (129), (56), (2), (1)
Host Mouse
(268), (68), (21), (10)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BID antibody is un-conjugated
(20), (18), (13), (8), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunofluorescence (IF), Western Blotting (WB)
(280), (166), (108), (63), (34), (34), (25), (18), (12), (11), (9), (3), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID Antibody (ABIN968577) Images
Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse Bid aa. 50-62
Clone 7-Bid
Isotype IgG1

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN968577) Images back to top
Antigen
Alternative Name Bid (BID Antibody Abstract)
Background Members of the Bcl-2 protein family function to inhibit (Bcl-2, Bcl-XL, Mcl-1, A1) or promote (Bax, Bak, Bcl-Ls, Bad) apoptosis. Bid is a Bcl-2 family death agonist that heterodimerizes with either agonists (Bax) or antagonists (Bcl-2). Bid contains a centrally located BH3 domain that allows interaction with the BH1 domain of Bax and Bcl-2. However, Bid does not contain other domains commonly found in Bcl-2 family members, such as BH1, BH2, or BH4. In addition, Bid does not contain a C-terminal hydrophobic region that is characteristic of membrane-bound Bcl-2 family members. Singlet oxygen-induced apoptosis in human leukemia cells and Fas-induced apoptosis involve caspase-8 cleavage of Bid, which produces a 15 kDa C-terminal fragment and a 6.5 kDa N-terminal fragment. The C-terminal fragment translocates to the mitochondria and promotes the release of cytochrome C through a mechanism that might involve destabilization of the mitochondrial membrane. Thus, the proapoptotic role of Bid may involve mitochondrial membrane destabilization, as well as multiple protein-protein interactions.
Molecular Weight 23 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN968577) Images back to top
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN968577) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-BID Antibody (ABIN968577)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Product cited in:

Devarajan, De Leon, Talasazan, Schoenfeld, Davidowitz, Burk: "The von Hippel-Lindau gene product inhibits renal cell apoptosis via Bcl-2-dependent pathways." in: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 40599-605, 2001

Gómez-Angelats, Cidlowski: "Protein kinase C regulates FADD recruitment and death-inducing signaling complex formation in Fas/CD95-induced apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 44944-52, 2001

Zhuang, Demirs, Kochevar: "p38 mitogen-activated protein kinase mediates bid cleavage, mitochondrial dysfunction, and caspase-3 activation during apoptosis induced by singlet oxygen but not by hydrogen peroxide." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 25939-48, 2000

Kudla, Montessuit, Eskes, Berrier, Martinou, Ghazi, Antonsson: "The destabilization of lipid membranes induced by the C-terminal fragment of caspase 8-cleaved bid is inhibited by the N-terminal fragment." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 22713-8, 2000

Wang, Yin, Chao, Milliman, Korsmeyer: "BID: a novel BH3 domain-only death agonist." in: Genes & development, Vol. 10, Issue 22, pp. 2859-69, 1996

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN968577) back to top
Supplier Images
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 50-62) (ABIN968577) Western blot analysis of Bid on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB...
Immunofluorescence (IF) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 50-62) (ABIN968577) Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).
 image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 50-62) (ABIN968577) anti-BH3 Interacting Domain Death Agonist (BID) (AA 50-62) antibody (Image 3)