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anti-Human BID antibody for Western Blotting

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist-like
  • BH3-interacting domain death agonist-like
  • BID
  • bida
  • bid
  • Bid
  • LOC100355991
  • LOC100730546
Epitope
AA 68-103, BH3 Domain
51
43
37
19
17
15
14
11
10
8
6
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
322
153
68
5
3
2
1
Host
Rabbit
297
96
23
9
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
327
187
129
72
50
41
32
21
14
12
11
3
2
1
1
1
1
1
1
Options
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Catalog No. ABIN388099
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0238638 ABIN389523 IHC (p) WB Rabbit Ig pSer65 Log in to see Polyclonal 5
3.0238638 ABIN222962 IP IHC WB Rabbit IgG Log in to see Polyclonal 6
3.0238638 ABIN1532322 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.0238638 ABIN4284616 WB Rabbit AA 120-180 Log in to see Polyclonal 1
3.0238638 ABIN250381 ELISA WB Goat C-Term, all Isoforms Log in to see Polyclonal 1
3.0238638 ABIN499461 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 1
3.0238638 ABIN499463 EIA WB Rabbit IgG Middle Region Log in to see Polyclonal 2
3.0238638 ABIN968577 IF WB Mouse IgG1 AA 50-62 Log in to see 7-Bid 5
3.0238638 ABIN968686 IF WB Mouse IgG1 AA 46-168 Log in to see 40-Bid 3
3.0238638 ABIN968979 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 3C5 2
3.0238638 ABIN153045 IHC (p) IHC WB Rabbit AA 60-120 Log in to see Polyclonal 2
3.0238638 ABIN4284621 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
3.0238638 ABIN4284618 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
3.0238638 ABIN336094 EIA WB Mouse IgG1 AA 1-195, N-Term Log in to see 4D3
3.0238638 ABIN1105530 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3C5 2
3.0238638 ABIN3042321 ICC IHC (p) WB Rabbit IgG AA 1-195 Log in to see Polyclonal
3.0238638 ABIN2788659 IF WB Rabbit C-Term Log in to see Polyclonal
3.0238638 ABIN3181773 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0238638 ABIN3183516 ELISA IHC WB Rabbit IgG Ser179 Log in to see Polyclonal
3.0238638 ABIN4284615 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 3

Top referenced anti-BID antibody for Western Blotting

Similar anti-BID Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 47 Antibodies 159 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 11 Antibodies
Flow Cytometry (FACS) 6 Antibodies 15 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 27 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Western Blotting (WB) 112 Antibodies
Cellular Assay (CA) 1 Antibodies
ELISA (Capture) 1 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Epitope AA 68-103, BH3 Domain
(51), (43), (37), (19), (17), (15), (14), (11), (10), (8), (6), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(322), (153), (68), (5), (3), (2), (1)
Host Rabbit
(297), (96), (23), (9)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(327), (187), (129), (72), (50), (41), (32), (21), (14), (12), (11), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID Antibody (ABIN388099) Images
Specificity This Bid antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78~108 amino acids from the BH3 domain of human Bid.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This Bid antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-103 AA from human Bid.
Clone RB0957-RB0958
Isotype Ig

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN388099) Images back to top
Antigen
Alternative Name Bid (BID Antibody Abstract)
Background Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.
Synonyms: BH3-interacting domain death agonist, BID, p22 BID
Molecular Weight 21994 DA
Gene ID 637
UniProt P55957
Research Area Apoptosis/Necrosis, Cancer, Cell Structure
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN388099) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN388099) Images back to top
Format Liquid
Concentration 1.855 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BID Antibody (ABIN388099)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Product cited in:

Pedrini, Sau, Guareschi, Bogush, Brown, Naniche, Kia, Trotti, Pasinelli: "ALS-linked mutant SOD1 damages mitochondria by promoting conformational changes in Bcl-2." in: Human molecular genetics, Vol. 19, Issue 15, pp. 2974-86, 2010

Background publications

Wang, Ryter, Dai, Tang, Watkins, Yin, Song, Choi: "Necrotic cell death in response to oxidant stress involves the activation of the apoptogenic caspase-8/bid pathway." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29184-91, 2003

Degli Esposti, Ferry, Masdehors, Boutin, Hickman, Dive: "Post-translational modification of Bid has differential effects on its susceptibility to cleavage by caspase 8 or caspase 3." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15749-57, 2003

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN388099) back to top
Supplier Images
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 68-103) (ABIN388099) The anti-Bid BH3 domain Pab (ABIN388099) is used in Western blot to detect Bid BH3 in...
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 68-103) (ABIN388099) Western blot analysis of anti-hBid-BH3 Pab (ABIN388099) in mouse lung tissue lysates ...
Immunohistochemistry (IHC) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 68-103) (ABIN388099) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...