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anti-Human BID antibody for Western Blotting

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • DKFZp469E066
  • FP497
  • fp497
  • si:ch211-238n5.6
  • xbid
Reactivity
Human
274
129
56
2
1
Host
Rabbit
267
69
21
10
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
20
18
13
8
7
7
3
3
3
3
3
3
3
3
3
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
280
166
107
63
34
33
26
18
12
11
9
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN222962
$ 283.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12502767 ABIN1532322 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12502767 ABIN388099 IHC (p) WB Rabbit Ig AA 68-103, BH3 Domain Log in to see Polyclonal 3
0.12502767 ABIN389523 IHC (p) WB Rabbit Ig pSer65 Log in to see Polyclonal 5
0.12502767 ABIN499461 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.12502767 ABIN499463 EIA WB Rabbit IgG Middle Region Log in to see Polyclonal 2
0.12502767 ABIN968577 IF WB Mouse IgG1 AA 50-62 Log in to see 7-Bid 5
0.12502767 ABIN968686 IF WB Mouse IgG1 AA 46-168 Log in to see 40-Bid 3
0.12502767 ABIN968979 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 3C5 2
0.12502767 ABIN336094 EIA WB Mouse IgG1 AA 1-195, N-Term Log in to see 4D3
0.12502767 ABIN1105530 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3C5 2
0.12502767 ABIN189116 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG Log in to see Y8
0.12502767 ABIN2788659 IF WB Rabbit C-Term Log in to see Polyclonal
0.12502767 ABIN3042321 ICC IHC (p) WB Rabbit IgG AA 1-195 Log in to see Polyclonal
0.12502767 ABIN1996891 IP ELISA WB Mouse IgG2a AA 1-195 Log in to see 3C4C2
0.12502767 ABIN1996893 FACS IF ELISA WB Mouse IgG1 AA 1-195 Log in to see 10
0.12502767 ABIN2465182 ELISA WB Goat C-Term Log in to see Polyclonal
0.12502767 ABIN2705594 WB Rabbit Center Log in to see Polyclonal
0.12502767 ABIN1996899 IP ELISA WB Rabbit IgG AA 1-195 Log in to see 2
0.12502767 ABIN1871288 IHC WB Rabbit IgG Log in to see Polyclonal
0.12502767 ABIN2855009 IP WB Rabbit IgG Center Log in to see Polyclonal

Top referenced anti-BID antibody for Western Blotting

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Application / Reactivity Human
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ELISA 139 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 13 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 98 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 47 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Immunostaining (ISt) 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
SDS-PAGE (SDS) 1 Antibodies
Western Blotting (WB) 228 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Reactivity Human
(274), (129), (56), (2), (1)
Host Rabbit
(267), (69), (21), (10)
Clonality
Polyclonal
Conjugate This BID antibody is un-conjugated
(20), (18), (13), (8), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(280), (166), (107), (63), (34), (33), (26), (18), (12), (11), (9), (3), (2), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID Antibody (ABIN222962) Images
Blocking Peptide Blocking peptide for this product available: ABIN412616
Specificity The antibody detects 22 kDa human Bid .
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 37 of human Bid
Isotype IgG

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN222962) Images back to top
Antigen
Alternative Name Bid (BID Antibody Abstract)
Background Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed throμgh Fas.
Synonyms: FP497 , MGC42355, MGC15319
Gene ID 637
UniProt P55957
Research Area Cancer, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN222962) Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL), Immunoprecipitation (5-10 μg/mL), and Immunohistochemistry (20-40 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN222962) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-Bid polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-BID Antibody (ABIN222962)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Product cited in:

Liang, Yang, Huang, Lin, Chou, Chen, Pan: "Antegrade retrieval of ureteral stents through an 8-French percutaneous nephrostomy route." in: AJR. American journal of roentgenology, Vol. 191, Issue 5, pp. 1530-5, 2008

Chen, Huang, Wang: "Deficiency of Bim in dendritic cells contributes to overactivation of lymphocytes and autoimmunity." in: Blood, Vol. 109, Issue 10, pp. 4360-7, 2007

Huang, Masselli, Frisch, Hunton, Jiang, Wang: "Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29401-13, 2007

Ju, Valencia, Pang, Ke, Gao, Dong, Liu: "Proteome-wide identification of family member-specific natural substrate repertoire of caspases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 36, pp. 14294-9, 2007

Kulik, Carson, Vomastek, Overman, Gooch, Srinivasula, Alnemri, Nunez, Weber: "Tumor necrosis factor alpha induces BID cleavage and bypasses antiapoptotic signals in prostate cancer LNCaP cells." in: Cancer research, Vol. 61, Issue 6, pp. 2713-9, 2001

Wang, Wieckowski, Goldstein, Gastman, Rabinovitz, Gambotto, Li, Fang, Yin, Rabinowich: "Resistance to granzyme B-mediated cytochrome c release in Bak-deficient cells." in: The Journal of experimental medicine, Vol. 194, Issue 9, pp. 1325-37, 2001

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN222962) back to top
Supplier Images
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN222962) Western blot analysis of Bid expression in Jurkat cell lysate.