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|Application / Reactivity||Human|
|Cellular Assay (CA)||1 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||11 Antibodies|
|Flow Cytometry (FACS)||13 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies|
|Immunofluorescence (IF)||26 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||97 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||47 Antibodies|
|Immunoprecipitation (IP)||27 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies|
|SDS-PAGE (SDS)||1 Antibodies|
|Western Blotting (WB)||227 Antibodies|
|Antigen||BH3 Interacting Domain Death Agonist (BID) Antibodies|
|Conjugate||This BID antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-BID AntibodyTarget Details BID Application Details Handling References for anti-BID Antibody (ABIN222962) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412616|
|Specificity||The antibody detects 22 kDa human Bid .|
|Immunogen||Synthetic peptide surrounding amino acid 37 of human Bid|
Target Details BIDProduct Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN222962) Images back to top
|Alternative Name||Bid (BID Antibody Abstract)|
Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed throμgh Fas.
Synonyms: FP497 , MGC42355, MGC15319
|Research Area||Cancer, Apoptosis/Necrosis|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN222962) Images back to top
Western blot analysis (0.5-4 μg/mL), Immunoprecipitation (5-10 μg/mL), and Immunohistochemistry (20-40 μg/mL).
However, the optimal conditions should be determined individually.
Country of Animal Origin: China
|Restrictions||For Research Use only|
HandlingProduct Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN222962) Images back to top
|Buffer||100 μg (0.5 mg/mL) affinity purified rabbit anti-Bid polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-BID Antibody (ABIN222962)Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
|Product cited in:||
Liang, Yang, Huang et al.: "Antegrade retrieval of ureteral stents through an 8-French percutaneous nephrostomy route." in: AJR. American journal of roentgenology, Vol. 191, Issue 5, pp. 1530-5, 2008 (PubMed).
Ju, Valencia, Pang et al.: "Proteome-wide identification of family member-specific natural substrate repertoire of caspases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 36, pp. 14294-9, 2007 (PubMed).
Huang, Masselli, Frisch et al.: "Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29401-13, 2007 (PubMed).
Chen, Huang, Wang: "Deficiency of Bim in dendritic cells contributes to overactivation of lymphocytes and autoimmunity." in: Blood, Vol. 109, Issue 10, pp. 4360-7, 2007 (PubMed).
Wang, Wieckowski, Goldstein et al.: "Resistance to granzyme B-mediated cytochrome c release in Bak-deficient cells." in: The Journal of experimental medicine, Vol. 194, Issue 9, pp. 1325-37, 2001 (PubMed).
Kulik, Carson, Vomastek et al.: "Tumor necrosis factor alpha induces BID cleavage and bypasses antiapoptotic signals in prostate cancer LNCaP cells." in: Cancer research, Vol. 61, Issue 6, pp. 2713-9, 2001 (PubMed).
ImagesProduct Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN222962) back to top