We are preparing the requested document. Please wait, this may take a while...!
|Antigen||BH3 Interacting Domain Death Agonist (BID) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This BID antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-BID AntibodyTarget Details BID Application Details Handling References for anti-BID antibody (ABIN356817) Images
|Specificity||This antibody is specific to BID (non pSer61).|
|Purification||Protein A Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S61 of mouse BID.|
Target Details BIDProduct Details anti-BID Antibody Application Details Handling References for anti-BID antibody (ABIN356817) Images back to top
|Alternative Name||BID (BID Antibody Abstract)|
|Background||Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.Synonyms: BH3-interacting domain death agonist, p22 BID|
|Gene ID||12122, 10090|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-BID Antibody Target Details BID Handling References for anti-BID antibody (ABIN356817) Images back to top
ELISA: 1/1,000. Western blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BID Antibody Target Details BID Application Details References for anti-BID antibody (ABIN356817) Images back to top
|Buffer||PBS containing 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-BID antibody (ABIN356817)Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Kaufmann, Jost, Pellegrini, Puthalakath, Gugasyan, Gerondakis, Cretney, Smyth, Silke, Hakem, Bouillet, Mak, Dixit, Strasser: "Fatal hepatitis mediated by tumor necrosis factor TNFalpha requires caspase-8 and involves the BH3-only proteins Bid and Bim." in: Immunity, Vol. 30, Issue 1, pp. 56-66, 2009
Ziporen, Donin, Shmushkovich, Gross, Fishelson: "Programmed necrotic cell death induced by complement involves a Bid-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 1, pp. 515-21, 2008
Mandal, Crusio, Meng, Liu, Kinsella, Clark, Takeuchi, Aifantis: "Regulation of lymphocyte progenitor survival by the proapoptotic activities of Bim and Bid." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 52, pp. 20840-5, 2008
ImagesProduct Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID antibody (ABIN356817) back to top