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anti-Mouse (Murine) BID antibody for Enzyme Immunoassay

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • DKFZp469E066
  • FP497
  • fp497
  • si:ch211-238n5.6
  • xbid
Epitope
pSer61
50
43
34
17
15
14
13
11
9
8
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
305
136
62
4
2
2
1
Host
Rabbit
276
90
21
10
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
310
186
118
71
40
34
30
19
13
13
11
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN356817
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088587624 ABIN499461 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.00088587624 ABIN499463 EIA WB Rabbit IgG Middle Region Log in to see Polyclonal 2
0.00088587624 ABIN372916 EIA IHC (fro) IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.00088587624 ABIN372917 EIA IHC (p) WB Rabbit IgG Middle Region Log in to see Polyclonal

Top referenced anti-BID antibody for Enzyme Immunoassay

Similar anti-BID Antibodies

Application / Reactivity Mouse (Murine)
ELISA 48 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Western Blotting (WB) 106 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Epitope pSer61
(50), (43), (34), (17), (15), (14), (13), (11), (9), (8), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(305), (136), (62), (4), (2), (2), (1)
Host Rabbit
(276), (90), (21), (10)
Clonality
Polyclonal
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(310), (186), (118), (71), (40), (34), (30), (19), (13), (13), (11), (3), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID Antibody (ABIN356817) Images
Specificity This antibody is specific to BID (non pSer61).
Purification Protein A Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S61 of mouse BID.
Isotype Ig

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN356817) Images back to top
Antigen
Alternative Name BID (BID Antibody Abstract)
Background Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.Synonyms: BH3-interacting domain death agonist, p22 BID
Gene ID 12122, 10090
UniProt P70444
Research Area Cancer
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN356817) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN356817) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-BID Antibody (ABIN356817)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Background publications

Kaufmann, Jost, Pellegrini, Puthalakath, Gugasyan, Gerondakis, Cretney, Smyth, Silke, Hakem, Bouillet, Mak, Dixit, Strasser: "Fatal hepatitis mediated by tumor necrosis factor TNFalpha requires caspase-8 and involves the BH3-only proteins Bid and Bim." in: Immunity, Vol. 30, Issue 1, pp. 56-66, 2009

Ziporen, Donin, Shmushkovich, Gross, Fishelson: "Programmed necrotic cell death induced by complement involves a Bid-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 1, pp. 515-21, 2008

Mandal, Crusio, Meng, Liu, Kinsella, Clark, Takeuchi, Aifantis: "Regulation of lymphocyte progenitor survival by the proapoptotic activities of Bim and Bid." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 52, pp. 20840-5, 2008

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN356817) back to top
Supplier Images
 image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (pSer61) (ABIN356817) anti-BH3 Interacting Domain Death Agonist (BID) (pSer61) antibody