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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||57 Antibodies||154 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies||10 Antibodies|
|Flow Cytometry (FACS)||7 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||7 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||27 Antibodies|
|Immunohistochemistry (IHC)||55 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||10 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||48 Antibodies|
|Immunoprecipitation (IP)||26 Antibodies|
|Western Blotting (WB)||135 Antibodies|
|Cell Culture (CC)||1 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Antigen||BH3 Interacting Domain Death Agonist (BID) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This BID antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-BID AntibodyTarget Details BID Application Details Handling Images
|Specificity||This antibody reacts to BH3 Interacting Domain Death Agonist (BID).|
Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
|Purification||Ion Exchange Chromatography.|
|Immunogen||Synthetic peptide - KLH conjugated corresponding to 14 amino acids near the carboxy- terminus of Human Bid.|
Target Details BIDProduct Details anti-BID Antibody Application Details Handling Images back to top
|Alternative Name||BID (BID Antibody Abstract)|
|Background||Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.Synonyms: BH3-interacting domain death agonist, p22 BID|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-BID Antibody Target Details BID Handling Images back to top
ELISA. Western blot: 0.5-1.0 μg/mL. Immunohistochemistry on Frozen Sections: 2 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BID Antibody Target Details BID Application Details Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
ImagesProduct Details anti-BID Antibody Target Details BID Application Details Handling back to top