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anti-Human BIRC5 antibody for Western Blotting

Recommended BIRC5 Antibody (supplied by: Log in to see )

Antigen
Baculoviral IAP Repeat-Containing 5 (BIRC5) Antibodies
  • AAC-11
  • AP14
  • API4
  • Api4
  • EPR-1
  • survivin40
  • TIAP
Epitope
C-Term
20
5
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
65
35
10
1
1
1
Host
Rabbit
57
22
3
Clonality
Polyclonal
Conjugate
This BIRC5 antibody is un-conjugated
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
59
24
20
14
13
7
5
3
2
2
1
1
1
Supplier
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Catalog No. ABIN500841
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN396525 WB Mouse IgG2a kappa AA 1-101 Log in to see 5B10 5
0.12303223 ABIN559893 IF IP RNAi ELISA WB Mouse IgG2a kappa AA 1-100, partial Log in to see 5B10 2
0.12303223 ABIN1107626 WB Mouse IgG1 Log in to see 2H5H2 3
0.12303223 ABIN3043195 WB Rabbit IgG AA 121-142, C-Term Log in to see Polyclonal
0.12303223 ABIN1449649 EIA IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2465183 ELISA WB Goat C-Term Log in to see Polyclonal
0.12303223 ABIN2452811 FACS IF WB Mouse IgG1 Log in to see 2D9
0.12303223 ABIN3042792 IHC (p) ELISA WB Rabbit IgG AA 1-142 Log in to see Polyclonal
0.12303223 ABIN969504 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 1H5
0.12303223 ABIN2163020 IF IHC ELISA WB Rabbit IgG Thr117 Log in to see Polyclonal
0.12303223 ABIN1875407 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2165471 ELISA WB Goat IgG C-Term Log in to see Polyclonal
0.12303223 ABIN947570 IF IP WB Rabbit AA 1-142, full length Log in to see Polyclonal
0.12303223 ABIN306760 WB Rabbit IgG C-Term Log in to see Polyclonal 2
0.12303223 ABIN306761 WB Rabbit IgG N-Term Log in to see Polyclonal 2
0.12303223 ABIN2428785 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2404306 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN513405 IF WB Mouse AA 1-142, full length Log in to see Polyclonal
0.12303223 ABIN513406 IF PLA WB Rabbit AA 1-142, full length Log in to see Polyclonal
0.12303223 ABIN1845166 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see

Top referenced anti-BIRC5 antibody for Western Blotting

Similar anti-BIRC5 Antibodies

Application / Reactivity Human
ELISA 16 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunohistochemistry (IHC) 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Western Blotting (WB) 48 Antibodies

General

Antigen Baculoviral IAP Repeat-Containing 5 (BIRC5) Antibodies
Epitope C-Term
(20), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(65), (35), (10), (1), (1), (1)
Host Rabbit
(57), (22), (3)
Clonality
Polyclonal
Conjugate This BIRC5 antibody is un-conjugated
(2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(59), (24), (20), (14), (13), (7), (5), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BIRC5 Antibody

Target Details BIRC5 Application Details Handling References for anti-BIRC5 Antibody (ABIN500841) Images
Specificity This antibody detects IAP4 / BIRC5 at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Peptide affinity chromatography
Immunogen Survivin antibody was raised against a peptide corresponding to amino acids near the carboxy-terminus of human survivin.
Isotype IgG

Target Details BIRC5

Product Details anti-BIRC5 Antibody Application Details Handling References for anti-BIRC5 Antibody (ABIN500841) Images back to top
Antigen
Alternative Name IAP4 / BIRC5 (BIRC5 Antibody Abstract)
Background Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP (1-3). Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas (1,3).Synonyms: API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, Baculoviral IAP repeat-containing protein 5, Survivin
Gene ID 332
NCBI Accession NP_001159
Research Area Cancer, Apoptosis/Necrosis, Neurology
Pathways Apoptosis, Cell Division Cycle

Application Details

Product Details anti-BIRC5 Antibody Target Details BIRC5 Handling References for anti-BIRC5 Antibody (ABIN500841) Images back to top
Application Notes ELISA. Western blot: 0.5 to 1 μg/mL, an approximate 17 kDa band should be detected. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BIRC5 Antibody Target Details BIRC5 Application Details References for anti-BIRC5 Antibody (ABIN500841) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-BIRC5 Antibody (ABIN500841)

Product Details anti-BIRC5 Antibody Target Details BIRC5 Application Details Handling Images back to top
Background publications

Ambrosini, Adida, Altieri: "A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma." in: Nature medicine, Vol. 3, Issue 8, pp. 917-21, 1997

Images

Product Details anti-BIRC5 Antibody Target Details BIRC5 Application Details Handling References for anti-BIRC5 Antibody (ABIN500841) back to top
Supplier Images
Immunofluorescence (IF) image for anti-BIRC5 antibody (Baculoviral IAP Repeat-Containing 5) (C-Term) (ABIN500841) Immunocytochemistry staining of MOLT4 cells using AP30844PU-N at 10 μg/ml.
Western Blotting (WB) image for anti-BIRC5 antibody (Baculoviral IAP Repeat-Containing 5) (C-Term) (ABIN500841) Western blot analysis of survivin in MOLT4 cell lysate in the absence (A) or presence...