anti-Human BIRC5 antibody for Western Blotting

Recommended BIRC5 Antibody (supplied by: Log in to see )

Antigen
Baculoviral IAP Repeat-Containing 5 (BIRC5) Antibodies
  • API4
  • EPR-1
  • AAC-11
  • Api4
  • TIAP
  • survivin40
  • AP14
  • baculoviral IAP repeat containing 5
  • baculoviral IAP repeat-containing 5-like
  • baculoviral IAP repeat-containing 5
  • BIRC5
  • LOC696283
  • Birc5
Epitope
AA 1-101
20
10
5
4
3
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
69
31
9
1
1
1
Host
Mouse
54
26
4
Clonality
Monoclonal
Conjugate
This BIRC5 antibody is un-conjugated
3
2
2
1
1
Application
Western Blotting (WB)
60
24
22
13
13
13
5
3
2
2
2
1
1
1
Options
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Catalog No. ABIN396525
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1588345 ABIN3043195 WB Rabbit IgG AA 121-142, C-Term Log in to see Polyclonal
3.1588345 ABIN4900855 FACS IHC SimWes WB Goat IgG Log in to see Polyclonal 2
3.1588345 ABIN3042792 IHC (p) ELISA WB Rabbit IgG AA 1-142 Log in to see Polyclonal
3.1588345 ABIN559893 IF IP RNAi ELISA WB Mouse IgG2a kappa AA 1-100, partial Log in to see 5B10 2
3.1588345 ABIN1107626 WB Mouse IgG1 Log in to see 2H5H2 3
3.1588345 ABIN2452811 FACS IF WB Mouse IgG1 Log in to see 2D9
3.1588345 ABIN2465183 ELISA WB Goat C-Term Log in to see Polyclonal
3.1588345 ABIN1449649 EIA IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.1588345 ABIN1875407 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.1588345 ABIN969504 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 1H5
3.1588345 ABIN306761 WB Rabbit IgG N-Term Log in to see Polyclonal 2
3.1588345 ABIN306760 WB Rabbit IgG C-Term Log in to see Polyclonal 2
3.1588345 ABIN947570 IF IP WB Rabbit AA 1-142, full length Log in to see Polyclonal
3.1588345 ABIN2404306 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.1588345 ABIN513405 IF WB Mouse AA 1-142, full length Log in to see Polyclonal
3.1588345 ABIN513406 IF PLA WB Rabbit AA 1-142, full length Log in to see Polyclonal
3.1588345 ABIN1845166 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see
3.1588345 ABIN2428785 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN401046 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 1
1 ABIN272115 IF IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-BIRC5 antibody for Western Blotting

Similar anti-BIRC5 Antibodies

Application / Reactivity Human
ELISA 18 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 13 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Simple Western (SimWes) 1 Antibodies

General

Antigen Baculoviral IAP Repeat-Containing 5 (BIRC5) Antibodies
Epitope AA 1-101
(20), (10), (5), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(69), (31), (9), (1), (1), (1)
Host Mouse
(54), (26), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BIRC5 antibody is un-conjugated
(3), (2), (2), (1), (1)
Application Western Blotting (WB)
(60), (24), (22), (13), (13), (13), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-BIRC5 Antibody

Target Details BIRC5 Application Details Handling ProductDetails: References for anti-BIRC5 antibody (ABIN396525) Images
Specificity BIRC5 (NP_001159, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen BIRC5 (NP_001159, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 5B10
Isotype IgG2a kappa

Target Details BIRC5

Product Details anti-BIRC5 Antibody Application Details Handling ProductDetails: References for anti-BIRC5 antibody (ABIN396525) Images back to top
Antigen
Alternative Name BIRC5 (BIRC5 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 16390 DA
Gene ID 12406
NCBI Accession NP_001159
Research Area Cancer, Cell Structure
Pathways Apoptosis, Cell Division Cycle

Application Details

Product Details anti-BIRC5 Antibody Target Details BIRC5 Handling ProductDetails: References for anti-BIRC5 antibody (ABIN396525) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.

Restrictions For Research Use only

Handling

Product Details anti-BIRC5 Antibody Target Details BIRC5 Application Details ProductDetails: References for anti-BIRC5 antibody (ABIN396525) Images back to top
Concentration 0.38mg/ml, 0.5mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

ProductDetails: References for anti-BIRC5 antibody (ABIN396525)

Product Details anti-BIRC5 Antibody Target Details BIRC5 Application Details Handling Images back to top
Background publications

Niu, Cheng, Ren, Yang: "Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis." in: FEBS letters, Vol. 584, Issue 16, pp. 3519-24, 2010

Yoshida, Zhang, Rivera Rosado, Chen, Khan, Moon, Zhang: "Blockade of Rac1 activity induces G1 cell cycle arrest or apoptosis in breast cancer cells through downregulation of cyclin D1, survivin, and X-linked inhibitor of apoptosis protein." in: Molecular cancer therapeutics, Vol. 9, Issue 6, pp. 1657-68, 2010

Yang, Sun, Cao, Liu: "Survivin downregulation by siRNA/cationic liposome complex radiosensitises human hepatoma cells in vitro and in vivo." in: International journal of radiation biology, Vol. 86, Issue 6, pp. 445-57, 2010

Liu, Wu, Chen, Ter-Minassian, Asomaning, Zhai, Wang, Su, Heist, Kulke, Lin, Liu, Christiani: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010

Naumnik, Nilklińska, Ossolińska, Chyczewska: "Serum levels of HMGB1, survivin, and VEGF in patients with advanced non-small cell lung cancer during chemotherapy." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 47, Issue 4, pp. 703-9, 2010

Images

Product Details anti-BIRC5 Antibody Target Details BIRC5 Application Details Handling ProductDetails: References for anti-BIRC5 antibody (ABIN396525) back to top
Supplier Images
Western Blotting (WB) image for anti-BIRC5 antibody (Baculoviral IAP Repeat-Containing 5) (AA 1-101) (ABIN396525) Western Blot analysis of BIRC5 expression in transfected 293T cell line by BIRC5 mono...
Immunohistochemistry (IHC) image for anti-BIRC5 antibody (Baculoviral IAP Repeat-Containing 5) (AA 1-101) (ABIN396525) anti-Baculoviral IAP Repeat-Containing 5 (BIRC5) (AA 1-101) antibody (Image 2)