anti-Human CAPNL1 antibody for Immunocytochemistry

Recommended CAPNL1 Antibody (supplied by: Log in to see )

Antigen
Calpain 1, Large Subunit (CAPNL1) Antibodies
  • si:ch211-203k12.4
  • si:dkeyp-101g1.3
  • Capa-1
  • Capa1
  • mu-calpin
  • CANP
  • CANP1
  • CANPL1
  • muCANP
  • muCL
  • calpain 1, (mu/I) large subunit
  • calpain 1, (mu/I) large subunit b
  • calpain 1
  • capn1
  • capn1b
  • Capn1
  • CAPN1
Epitope
AA 312-326, Middle Region
5
4
3
3
3
3
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
54
17
16
3
2
2
2
2
2
2
1
1
Host
Rabbit
33
21
6
Clonality
Polyclonal
Conjugate
This CAPNL1 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
59
33
18
6
6
5
5
3
1
1
Options
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Catalog No. ABIN3044292
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1858230 ICC IHC IP WB Rabbit IgG AA 426-676 Log in to see Polyclonal

Top referenced anti-CAPNL1 antibody for Immunocytochemistry

Similar anti-CAPNL1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 8 Antibodies 8 Antibodies 29 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 1 Antibodies 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 10 Antibodies 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 1 Antibodies 1 Antibodies
Western Blotting (WB) 17 Antibodies 18 Antibodies
Flow Cytometry (FACS) 1 Antibodies

General

Antigen Calpain 1, Large Subunit (CAPNL1) Antibodies
Epitope AA 312-326, Middle Region
(5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(54), (17), (16), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(33), (21), (6)
Clonality
Polyclonal
Conjugate This CAPNL1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(59), (33), (18), (6), (6), (5), (5), (3), (1), (1)
Pubmed 2 references available
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Product Details anti-CAPNL1 Antibody

Target Details CAPNL1 Application Details Handling ProductDetails: References for anti-CAPNL1 antibody (ABIN3044292) Images
Purpose Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Sequence EWNNVDPYER DQLRV
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: calpain 1,(mu/I) large subunit
Protein Name: Calpain-1 catalytic subunit
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1(312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids.
Isotype IgG

Target Details CAPNL1

Product Details anti-CAPNL1 Antibody Application Details Handling ProductDetails: References for anti-CAPNL1 antibody (ABIN3044292) Images back to top
Antigen
Alternative Name CAPN1 (CAPNL1 Antibody Abstract)
Background Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I(mu-calpain) and calpain II(m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L(large, catalytic, 80 kD) and S(small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1(CAPN1) and L2. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy(e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CANPL1 to chromosome 11.

Synonyms: Ca2 activated neutral protease antibody|Calcium activated neutral proteinase 1 antibody|Calcium activated neutral proteinase antibody|Calcium activated neutral proteinase small subunit antibody|Calcium dependent protease small subunit 1 antibody|Calcium dependent protease small subunit antibody|Calcium-activated neutral proteinase 1 antibody|Calpain 1(mu/I) large subunit antibody|Calpain 1 antibody|Calpain 1 catalytic subunit antibody|Calpain 1 large subunit antibody|Calpain Large Polypeptide L1 antibody|Calpain Large Polypeptide L1 antibody|Calpain mu type antibody|Calpain mu-type antibody|Calpain regulatory subunit antibody|Calpain small subunit 1 antibody|Calpain-1 catalytic subunit antibody|Calpain-1 large subunit antibody|Calpain1 antibody|CAN1_HUMAN antibody|CANP 1 antibody|CANP antibody|CANP small subunit antibody|CANPL 1 antibody|CANPL1 antibody|CAPN 1 antibody|CAPN1 antibody|Cell proliferation inducing protein 30 antibody|Cell proliferation-inducing gene 30 protein antibody|Micromolar Calpain antibody|Micromolar-calpain antibody|Mu Calpain antibody|muCANP antibody|muCL antibody
UniProt P07384
Pathways Apoptosis

Application Details

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Handling ProductDetails: References for anti-CAPNL1 antibody (ABIN3044292) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Application Details ProductDetails: References for anti-CAPNL1 antibody (ABIN3044292) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

ProductDetails: References for anti-CAPNL1 antibody (ABIN3044292)

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Application Details Handling Images back to top
Product cited in:

Chen, Tang, Gao, Xu, Jiang, Cao, Fu, Sun, Liu, Shi: "Fibrosis, adipogenesis, and muscle atrophy in congenital muscular torticollis." in: Medicine, Vol. 93, Issue 23, pp. e138, 2014

Mo, Chen, Li, Zheng, Ke, Deng, Li, Jiang, Wu, Wang, Wang, Yang, Cao: "Suppression of NHE1 by small interfering RNA inhibits HIF-1?-induced angiogenesis in vitro via modulation of calpain activity." in: Microvascular research, Vol. 81, Issue 2, pp. 160-8, 2011

Images

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Application Details Handling ProductDetails: References for anti-CAPNL1 antibody (ABIN3044292) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CAPNL1 antibody (Calpain 1, Large Subunit) (AA 312-326) (ABIN3044292) Anti-Calpain 1 antibody, IHC(P) IHC(P): Rat Lung Tissue
Immunohistochemistry (IHC) image for anti-CAPNL1 antibody (Calpain 1, Large Subunit) (AA 312-326) (ABIN3044292) Anti-Calpain 1 antibody, ICC ICC: HELA Cell
Western Blotting (WB) image for anti-CAPNL1 antibody (Calpain 1, Large Subunit) (AA 312-326) (ABIN3044292) Anti-Calpain 1 antibody, Western blotting Lane 1: Cell Lysate Lane 2: COLO320 Cell L...
Immunohistochemistry (IHC) image for anti-CAPNL1 antibody (Calpain 1, Large Subunit) (AA 312-326) (ABIN3044292) Anti-Calpain 1 antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue