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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||23 Antibodies||57 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||8 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||12 Antibodies|
|Immunohistochemistry (IHC)||11 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||14 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||51 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Antigen||Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This Caspase 4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-Caspase 4 AntibodyTarget Details Caspase 4 Application Details Handling References for anti-Caspase 4 Antibody (ABIN499561) Images
|Specificity||This antibody was raised against a 16 amino acid peptide from the amino-terminus of human Caspase-4.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Human Casp-4 / ICH-2 / ICE rel II / Mih1 (N-Terminus) Peptide|
Target Details Caspase 4Product Details anti-Caspase 4 Antibody Application Details Handling References for anti-Caspase 4 Antibody (ABIN499561) Images back to top
|Alternative Name||Caspase-4 (CASP4 Antibody Abstract)|
|Background||Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18 (2,3). Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells (4). More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease (5).Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Caspase 4 Antibody Target Details Caspase 4 Handling References for anti-Caspase 4 Antibody (ABIN499561) Images back to top
ELISA. Western Blot: Casp-4 antibody can be used for the detection of Caspase-4 at 1 μg/mL. Depending on cell lines or tissues used, other cleavage products may be observed. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Caspase 4 Antibody Target Details Caspase 4 Application Details References for anti-Caspase 4 Antibody (ABIN499561) Images back to top
|Buffer||PBS, 0.02 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Caspase 4 Antibody (ABIN499561)Product Details anti-Caspase 4 Antibody Target Details Caspase 4 Application Details Handling Images back to top
Martinon, Tschopp: "Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases." in: Cell, Vol. 117, Issue 5, pp. 561-74, 2004 (PubMed).
Gracie, Robertson, McInnes: "Interleukin-18." in: Journal of leukocyte biology, Vol. 73, Issue 2, pp. 213-24, 2003 (PubMed).
Kamens, Paskind, Hugunin et al.: "Identification and characterization of ICH-2, a novel member of the interleukin-1 beta-converting enzyme family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 15250-6, 1995 (PubMed).
Kuida, Lippke, Ku et al.: "Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme." in: Science (New York, N.Y.), Vol. 267, Issue 5206, pp. 2000-3, 1995 (PubMed).
ImagesProduct Details anti-Caspase 4 Antibody Target Details Caspase 4 Application Details Handling References for anti-Caspase 4 Antibody (ABIN499561) back to top