anti-Human Caspase 6 antibody for Enzyme Immunoassay

Recommended Caspase 6 Antibody (supplied by: Log in to see )

Antigen
Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) Antibodies
  • xCaspase-6
  • mch2
  • caspase-6
  • zgc:112960
  • CASP6
  • casp6
  • Mch2
  • mCASP-6
  • MCH2
  • caspase 6, apoptosis-related cysteine peptidase
  • caspase 6
  • caspase-6
  • Caspase-6
  • caspase-6-like
  • casp6
  • CASP6
  • CpipJ_CPIJ017008
  • Casp6
  • LOC100341546
  • LOC100517293
Epitope
pSer257
48
39
35
15
15
15
14
14
12
6
5
5
5
5
5
5
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
287
117
105
16
5
5
5
3
3
1
Host
Rabbit
275
21
19
Clonality
Polyclonal
Conjugate
This Caspase 6 antibody is un-conjugated
18
17
13
10
10
10
3
3
3
3
3
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
268
145
115
65
40
32
26
19
8
5
5
4
1
1
Supplier
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Catalog No. ABIN358091
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5552008 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
1 ABIN372895 EIA IHC (p) WB Rabbit IgG Cleavage Site Log in to see Polyclonal
1 ABIN5554663 EIA IF WB Rabbit IgG Intermediate Domain Log in to see Polyclonal
1 ABIN5554664 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-Caspase 6 antibody for Enzyme Immunoassay

Similar anti-Caspase 6 Antibodies

Application / Reactivity Human
ELISA 128 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 32 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 103 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 60 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 241 Antibodies

General

Antigen Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) Antibodies
Epitope pSer257
(48), (39), (35), (15), (15), (15), (14), (14), (12), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(287), (117), (105), (16), (5), (5), (5), (3), (3), (1)
Host Rabbit
(275), (21), (19)
Clonality
Polyclonal
Conjugate This Caspase 6 antibody is un-conjugated
(18), (17), (13), (10), (10), (10), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(268), (145), (115), (65), (40), (32), (26), (19), (8), (5), (5), (4), (1), (1)
Pubmed 5 references available
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Product Details anti-Caspase 6 Antibody

Target Details Caspase 6 Application Details Handling ProductDetails: References for anti-Caspase 6 antibody (ABIN358091) Images
Specificity This antibody detects Caspase-6 pSer257. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S257 of human caspase 6.
Isotype Ig

Target Details Caspase 6

Product Details anti-Caspase 6 Antibody Application Details Handling ProductDetails: References for anti-Caspase 6 antibody (ABIN358091) Images back to top
Antigen
Alternative Name Caspase-6 (CASP6 Antibody Abstract)
Background Caspase 6 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade.Synonyms: Apoptotic protease Mch-2, CASP-6, CASP6, MCH-2, MCH2
Molecular Weight 33310 Da
Gene ID 839, 9606
UniProt P55212
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Caspase 6 Antibody Target Details Caspase 6 Handling ProductDetails: References for anti-Caspase 6 antibody (ABIN358091) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 6 Antibody Target Details Caspase 6 Application Details ProductDetails: References for anti-Caspase 6 antibody (ABIN358091) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-Caspase 6 antibody (ABIN358091)

Product Details anti-Caspase 6 Antibody Target Details Caspase 6 Application Details Handling Images back to top
Background publications

Kalinin, Kalinin, Aho, Uitto, Aho: "Breaking the connection: caspase 6 disconnects intermediate filament-binding domain of periplakin from its actin-binding N-terminal region." in: The Journal of investigative dermatology, Vol. 124, Issue 1, pp. 46-55, 2005

Suzuki, Kusakai, Kishimoto, Shimojo, Miyamoto, Ogura, Ochiai, Esumi: "Regulation of caspase-6 and FLIP by the AMPK family member ARK5." in: Oncogene, Vol. 23, Issue 42, pp. 7067-75, 2004

Horowitz, Patterson, Guillozet-Bongaarts, Reynolds, Carroll, Weintraub, Bennett, Cryns, Berry, Binder: "Early N-terminal changes and caspase-6 cleavage of tau in Alzheimer's disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 36, pp. 7895-902, 2004

Schmeck, Gross, NGuessan, Hocke, Hammerschmidt, Mitchell, Rosseau, Suttorp, Hippenstiel: "Streptococcus pneumoniae-induced caspase 6-dependent apoptosis in lung epithelium." in: Infection and immunity, Vol. 72, Issue 9, pp. 4940-7, 2004

Méndez, Salas-Ocampo, Arias: "Caspases mediate processing of the capsid precursor and cell release of human astroviruses." in: Journal of virology, Vol. 78, Issue 16, pp. 8601-8, 2004

Images

Product Details anti-Caspase 6 Antibody Target Details Caspase 6 Application Details Handling ProductDetails: References for anti-Caspase 6 antibody (ABIN358091) back to top
Supplier Images
 image for anti-Caspase 6 antibody (Caspase 6, Apoptosis-Related Cysteine Peptidase) (pSer257) (ABIN358091) anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (pSer257) antibody
 image for anti-Caspase 6 antibody (Caspase 6, Apoptosis-Related Cysteine Peptidase) (pSer257) (ABIN358091) anti-Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (pSer257) antibody (Image 2)