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anti-Human Caspase 6 antibody for Flow Cytometry

Recommended Caspase 6 Antibody (supplied by: Log in to see )

Antigen
Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) Antibodies
  • casp6
  • CASP6
  • caspase-6
  • mCASP-6
  • MCH2
  • Mch2
  • mch2
  • xCaspase-6
  • zgc:112960
Reactivity
Human, Mouse (Murine), Rat (Rattus)
269
82
78
15
3
2
1
Host
Rabbit
266
19
16
Clonality
Monoclonal
Conjugate
This Caspase 6 antibody is un-conjugated
18
17
13
10
10
10
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
246
146
111
57
34
27
25
13
10
6
5
1
1
Supplier
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Available images

Catalog No. ABIN189207
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010485103 ABIN2687737 ICC FACS IP IHC WB Rabbit IgG Log in to see

Similar anti-Caspase 6 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 29 Antibodies 26 Antibodies
Flow Cytometry (FACS) 1 Antibodies 2 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 5 Antibodies
Immunofluorescence (IF) 14 Antibodies 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 36 Antibodies 35 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 62 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies

General

Antigen Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(269), (82), (78), (15), (3), (2), (1)
Host Rabbit
(266), (19), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 6 antibody is un-conjugated
(18), (17), (13), (10), (10), (10), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(246), (146), (111), (57), (34), (27), (25), (13), (10), (6), (5), (1), (1)
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Product Details anti-Caspase 6 Antibody

Target Details Caspase 6 Application Details Handling Images
Specificity The antibody should recognize the pro-form of Caspase-6.
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues of human Caspase-6 was used as immunogen.
Clone EP1325Y
Isotype IgG

Target Details Caspase 6

Product Details anti-Caspase 6 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Caspase-6 (CASP6 Antibody Abstract)
Background Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in theinitiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). Itexists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellularproteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of thecell membrane, and their cleavage results in subsequent cellular breakdown and death(4). Alternate Names: anti-Apoptosis Related Cysteine Protease antibody, anti-Apoptotic cysteine proteaseMCH2 antibody, anti-Apoptotic protease MCH 2 antibody, anti-Apoptotic proteaseMCH2 antibody, anti-Casp 6 antibody, anti-Casp6 antibody, anti-Caspase 6 apoptosisrelated cysteine protease antibody, anti-Caspase 6 precursor antibody, anti-Caspase6antibody, anti-Human cysteine protease Mch2 isoform alpha antibody, anti-Mch 2antibody, anti-Mch2 antibody.
Gene Symbol: CASP6
Gene ID 839, 12368, 83584
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Caspase 6 Antibody Target Details Caspase 6 Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 35kDa.
Recommended dilutions: Flow Cytometry 1:50, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for pro Caspase 6 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-Caspase 6 Antibody Target Details Caspase 6 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-Caspase 6 Antibody Target Details Caspase 6 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 6 antibody (Caspase 6, Apoptosis-Related Cysteine Peptidase) (ABIN189207) Western blot analysis on MCF-7 cell lysate using anti-Pro Caspase-6 RabMAb (ABIN18920...
Immunohistochemistry (IHC) image for anti-Caspase 6 antibody (Caspase 6, Apoptosis-Related Cysteine Peptidase) (ABIN189207) Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-P...
Immunofluorescence (IF) image for anti-Caspase 6 antibody (Caspase 6, Apoptosis-Related Cysteine Peptidase) (ABIN189207) Immunofluorescent staining of HeLa cells using anti-Pro-Caspase-6 RabMAb