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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||29 Antibodies||26 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||2 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||13 Antibodies||13 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies||22 Antibodies|
|Immunohistochemistry (IHC)||35 Antibodies||34 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Western Blotting (WB)||61 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Antigen||Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) Antibodies|
|Epitope||AA 7-27, N-Term Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Caspase 6 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Caspase 6 AntibodyTarget Details Caspase 6 Application Details Handling Images
|Sequence||FYRSREVLDP AEQYKMDHKR R|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Caspase-6(CASP6) detection. Tested with WB, IHC-P, IHC-F in Mouse, Rat.
Gene Name: caspase 6, apoptosis-related cysteine peptidase
Protein Name: Caspase-6
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of rat Caspase-6(P18)(7-27aa FYRSREVLDPAEQYKMDHKRR), different from the related mouse sequence by two amino acids.|
Target Details Caspase 6Product Details anti-Caspase 6 Antibody Application Details Handling Images back to top
|Alternative Name||CASP6 (CASP6 Antibody Abstract)|
|Background||Caspase 6 is an enzyme that in humans is encoded by the caspase 6 gene. This gene encodes a protein that is a member of the cysteine-aspartic acid protease(caspase) family. Using radiation hybrid mapping, the Caspase 6 gene is localized to human chromosome 4q25-q26. Caspase 6 functions as a downstream enzyme in the caspase activation cascade. And Caspase 6 can cleave lamin A to its signature apoptotic fragment.|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Caspase 6 Antibody Target Details Caspase 6 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Caspase 6 Antibody Target Details Caspase 6 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-Caspase 6 Antibody Target Details Caspase 6 Application Details Handling back to top