anti-Human Caspase 7 antibody for Western Blotting

Recommended Caspase 7 Antibody (supplied by: Log in to see )

Antigen
Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) Antibodies
  • xCaspase-7
  • caspase-7
  • caspase7
  • cmh-1
  • ice-lap3
  • mch3
  • CASP7
  • AI314680
  • CMH-1
  • ICE-IAP3
  • Mch3
  • mCASP-7
  • CASP-7
  • ICE-LAP3
  • LICE2
  • MCH3
  • Caspase-7
  • MCH3/SCA-2
  • SCA-2
  • caspase-7-like
  • caspase-7
  • caspase 7, apoptosis-related cysteine peptidase
  • caspase 7
  • LOC100068258
  • xCaspase-7
  • casp7
  • CASP7
  • Casp7
  • LOC100156777
  • LOC100101591
Reactivity
Human, Mouse (Murine), Rat (Rattus)
294
133
113
10
8
7
6
6
4
3
2
2
2
2
1
1
1
Host
Mouse
205
99
3
Clonality
Monoclonal
Conjugate
This Caspase 7 antibody is un-conjugated
14
12
9
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Simple Western (SimWes), Western Blotting (WB)
269
103
100
82
41
19
14
13
10
8
5
3
3
1
1
Supplier
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Catalog No. ABIN153012
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.347672 ABIN967332 IP WB Mouse IgG1, kappa full length Log in to see 10-1 6
3.347672 ABIN222955 IP IHC WB Rabbit IgG active Log in to see Polyclonal 10
3.347672 ABIN252490 IHC (p) WB Mouse IgG1 Log in to see 25B881-1 1
3.347672 ABIN264418 WB Mouse IgG Log in to see MCH3-5 3
3.347672 ABIN967551 IP WB Mouse IgG1, kappa AA 25-42 Log in to see B94-1 6
3.347672 ABIN968191 IF WB Mouse IgG2b AA 4-126 Log in to see CB11 5
3.347672 ABIN967333 IP WB Mouse IgG1, kappa full length Log in to see 10-1 6
3.347672 ABIN4288140 ELISA IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 1
3.347672 ABIN400716 IHC (p) WB Mouse IgG1 active Log in to see 25B881-1
3.347672 ABIN3042355 IHC (p) WB Rabbit IgG AA 117-198 Log in to see Polyclonal
3.347672 ABIN967331 IP WB Mouse IgG1, kappa Log in to see 8-1 6
3.347672 ABIN2787907 WB Rabbit N-Term Log in to see Polyclonal
3.347672 ABIN3043217 IHC (p) WB Rabbit IgG AA 237-255, Middle Region Log in to see Polyclonal
3.347672 ABIN2477917 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 3
3.347672 ABIN2477919 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 3
3.347672 ABIN1536084 IHC ELISA WB Rabbit IgG Cleaved-Asp198 Log in to see Polyclonal
3.347672 ABIN1536085 IHC ELISA WB Rabbit IgG Cleaved-Asp198 Log in to see Polyclonal
3.347672 ABIN1536107 ELISA WB Rabbit IgG Cleaved-Asp198 Log in to see Polyclonal
3.347672 ABIN4288137 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 2
3.347672 ABIN2855362 WB Rabbit IgG Center Log in to see Polyclonal

Top referenced anti-Caspase 7 antibody for Western Blotting

Similar anti-Caspase 7 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine)
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies 1 Antibodies 3 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Simple Western (SimWes) 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 259 Antibodies 116 Antibodies 95 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Immunoprecipitation (IP) 38 Antibodies 24 Antibodies 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 80 Antibodies 51 Antibodies 55 Antibodies
Immunohistochemistry (IHC) 96 Antibodies 47 Antibodies

General

Antigen Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(294), (133), (113), (10), (8), (7), (6), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(205), (99), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 7 antibody is un-conjugated
(14), (12), (9), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Simple Western (SimWes), Western Blotting (WB)
(269), (103), (100), (82), (41), (19), (14), (13), (10), (8), (5), (3), (3), (1), (1)
Pubmed 4 references available
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Product Details anti-Caspase 7 Antibody

Target Details Caspase 7 Application Details Handling ProductDetails: References for anti-Caspase 7 antibody (ABIN153012) Images
Purification Unpurified
Immunogen Full-length recombinant human Caspase 7 [UniProt# P55210]
Clone Mch3 1-1-11
Isotype IgG1 kappa

Target Details Caspase 7

Product Details anti-Caspase 7 Antibody Application Details Handling ProductDetails: References for anti-Caspase 7 antibody (ABIN153012) Images back to top
Antigen
Alternative Name Caspase-7 (CASP7 Antibody Abstract)
Background Gene Symbol: CASP7
Molecular Weight Theoretical MW: 35 kDa
Gene ID 840
UniProt P55210
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Caspase 7 Antibody Target Details Caspase 7 Handling ProductDetails: References for anti-Caspase 7 antibody (ABIN153012) Images back to top
Application Notes Western Blot 1:500-1:1000, Simple Western 1:500This Caspase 7 (Mch3 1-1-11) antibody is useful for Western blot, where a band can be seen at approx. 35 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Caspase 7 Antibody Procedure Guide for NB 500-206 Monoclonal anti-Caspase 7 Western Protocol
1) Prepare samples for a NuPAGE gel and heat them at 70?C for 10 minutes.
2) Run a 4-12 % NuPAGE Bis-Tris gel, loading 50-100 mg of whole cell extract per lane, for 45 minutes at 200 Volts.
) Soak transfer sponges and gel 15 minutes and nitrocellulose membrane 5 minutes prior to transfer.
) Transfer the proteins to the nitrocellulose membrane for 1 hour at 30 Volts, in 4 degrees C transfer buffer.
) Block the membrane in TBST + 5 % non-fat dry milk, for 1 hour at room temperature (RT), shaking gently.
) Rinse the membrane twice with TBST.
) Incubate the membrane in anti-Caspase 7 [NB 500-206], diluted 1:500-1:1,000 in TBS + 1 % BSA, for 90 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in TBS + 1 % BSA for 35 minutes at RT.
) Wash the membrane once for 15 minutes, then 3 times 5 minutes, in TBST at RT.
) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.*Positive control(s): HeLa whole cell extracts (cat# NB 800-PC1) Jurkat whole cell extracts (cat# NB 800-PC2)
Restrictions For Research Use only

Handling

Product Details anti-Caspase 7 Antibody Target Details Caspase 7 Application Details ProductDetails: References for anti-Caspase 7 antibody (ABIN153012) Images back to top
Format Liquid
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-Caspase 7 antibody (ABIN153012)

Product Details anti-Caspase 7 Antibody Target Details Caspase 7 Application Details Handling Images back to top
Product cited in:

Badr, Hafez, Agha, Shouman: "The Combination of α-Tocopheryl Succinate and Sodium Selenite on Breast Cancer: A Merit or a Demerit?" in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 4741694, 2016 (Sample species: Human). Further details: Western Blotting

Blanc-Brude, Mesri, Wall, Plescia, Dohi, Altieri: "Therapeutic targeting of the survivin pathway in cancer: initiation of mitochondrial apoptosis and suppression of tumor-associated angiogenesis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 9, Issue 7, pp. 2683-92, 2003

Background publications

Kirsch, Doseff, Chau, Lim, de Souza-Pinto, Hansford, Kastan, Lazebnik, Hardwick: "Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c." in: The Journal of biological chemistry, Vol. 274, Issue 30, pp. 21155-61, 1999

Fearnhead, Rodriguez, Govek, Guo, Kobayashi, Hannon, Lazebnik: "Oncogene-dependent apoptosis is mediated by caspase-9." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13664-9, 1998

Images

Product Details anti-Caspase 7 Antibody Target Details Caspase 7 Application Details Handling ProductDetails: References for anti-Caspase 7 antibody (ABIN153012) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (ABIN153012) Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [ABIN153012] - HeLa whole cell lysate.
Simple Western (SimWes) image for anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (ABIN153012) Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [ABIN153012] - Simple Western lane v...