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anti-Mouse (Murine) Caspase 7 antibody for Immunoprecipitation

Recommended Caspase 7 Antibody (supplied by: Log in to see )

Antigen
Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) Antibodies
  • AI314680
  • CASP-7
  • CASP7
  • Caspase-7
  • caspase-7
  • caspase7
  • cmh-1
  • CMH-1
  • ICE-IAP3
  • ice-lap3
  • ICE-LAP3
  • LICE2
  • mCASP-7
  • Mch3
  • mch3
  • MCH3
  • MCH3/SCA-2
  • SCA-2
  • xCaspase-7
Epitope
full length
32
29
12
7
7
6
6
6
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
285
108
85
8
7
4
4
3
3
2
1
1
Host
Mouse
203
90
4
Clonality
Monoclonal
Conjugate
This Caspase 7 antibody is un-conjugated
14
11
9
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
249
104
84
73
30
20
14
12
8
8
5
4
3
1
Supplier
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Catalog No. ABIN967332
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089465064 ABIN222955 IP IHC WB Rabbit IgG active Log in to see Polyclonal 10
0.00089465064 ABIN967333 IP WB Mouse IgG1, kappa full length Log in to see 10-1 6
0.00089465064 ABIN127027 IHC (p) IP WB Rabbit active Log in to see Polyclonal
0.00089465064 ABIN126920 IP WB Mouse IgG1 Log in to see B4-G2
0.00089465064 ABIN1871469 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089465064 ABIN222871 IP WB Mouse IgG1 Log in to see B4-G2
0.00089465064 ABIN1002039 IP IHC WB Rabbit IgG active Log in to see Polyclonal
0.00089465064 ABIN1818443 IP WB Mouse IgG2b N-Term Log in to see 4G2
0.00089465064 ABIN2404284 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089465064 ABIN1002038 IP WB Mouse IgG1 Log in to see B4-G2
0.00089465064 ABIN4288139 IHC IP WB Rabbit Large Subunit Log in to see Polyclonal
0.00089465064 ABIN4288136 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00089465064 ABIN4288137 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00089465064 ABIN2989799 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Caspase 7 antibody for Immunoprecipitation

Similar anti-Caspase 7 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 32 Antibodies 96 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Immunochromatography (IC) 1 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 11 Antibodies
Immunofluorescence (IF) 3 Antibodies 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies
Immunoprecipitation (IP) 15 Antibodies
Western Blotting (WB) 91 Antibodies
Flow Cytometry (FACS) 20 Antibodies

General

Antigen Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) Antibodies
Epitope full length
(32), (29), (12), (7), (7), (6), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(285), (108), (85), (8), (7), (4), (4), (3), (3), (2), (1), (1)
Host Mouse
(203), (90), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 7 antibody is un-conjugated
(14), (11), (9), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(249), (104), (84), (73), (30), (20), (14), (12), (8), (8), (5), (4), (3), (1)
Pubmed 6 references available
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Product Details anti-Caspase 7 Antibody

Target Details Caspase 7 Application Details Handling References for anti-Caspase 7 Antibody (ABIN967332) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human caspase-7 full-length recombinant protein
Clone 10-1
Isotype IgG1, kappa

Target Details Caspase 7

Product Details anti-Caspase 7 Antibody Application Details Handling References for anti-Caspase 7 Antibody (ABIN967332) Images back to top
Antigen
Alternative Name Caspase-7 (CASP7 Antibody Abstract)
Background The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspases are synthesized as inactive proenzymes containing three domains, that are processed into large and small subunits that associate to form the active enzyme. Processing can occur in apoptotic cells by either transactivation, self-proteolysis, or cleavage by another protease. While caspases share a common structure, there are some differences, such as the preferred substrate specificity. These sequence differences in specificity, as well as the size of the NH2 -terminal prodomains can be used to catagorize the caspases into functional groups including, apoptotic initiators (long prodomains), apoptotic executioners (short prodomains), and cytokine processors. Caspase-7, along with caspase-3 and -6 are members of the apoptotic executioners' group containing short prodomains, caspase-7 is structurally and functionally most similar to caspase-3. Upon induction of apoptosis, pro-caspase-7 (35 kDa) is first converted to a 32 kDa intermediate, which is further processed into active subunits consisting of 20 kDa and 11 kDa forms (Swiss-Prot P55210). Active caspase-7 has been shown to cleave the nuclear substrate PARP as well as the sterol regulatory element-binding protein 1 (SREBP-1). In cells undergoing Fas-mediated apoptosis in vivo, active caspase-7 has been shown to translocate from the cytosol to the mitochondrial and microsomal fractions, whereas caspase-3 remains cytosolic. This data supports the hypothesis that similar apoptotic executioners cleave distinct substrates in different cellular compartments.
The antibody recognizes human caspase-7. Full-length recombinant human caspase-7 protein was used as immunogen. The antibodies are routinely tested by western blot and immunoprecipitation analysis using Jurkat T cells (please refer to Table I for what forms of caspase-7 are identified in a particular application).
Synonyms: Mch3
Molecular Weight 20 kDa, 32 kDa, 35 kDa
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Caspase 7 Antibody Target Details Caspase 7 Handling References for anti-Caspase 7 Antibody (ABIN967332) Images back to top
Application Notes The antibody is recommended for western blot analysis (0.62-0.25 µg/ml) and immunoprecipitation (4 µg/200 µg cell lysate).Jurkat T cells (ATCC TIB-152) are recommended as a positive control for these applications. antibodies-online offers several monoclonal caspase-7 antibodies. A Jurkat and HepG2 model cell system was used to evaluate these antibodies, these results are summarized in the following table. However, actual bands observed could vary according to the cell model system or treatment used.
Comment

Related Products: ABIN967389, ABIN968537

Restrictions For Research Use only

Handling

Product Details anti-Caspase 7 Antibody Target Details Caspase 7 Application Details References for anti-Caspase 7 Antibody (ABIN967332) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-Caspase 7 Antibody (ABIN967332)

Product Details anti-Caspase 7 Antibody Target Details Caspase 7 Application Details Handling Images back to top
Product cited in:

Germain, Mathai, Shore: "BH-3-only BIK functions at the endoplasmic reticulum to stimulate cytochrome c release from mitochondria." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 18053-60, 2002

Thornberry, Rano, Peterson, Rasper, Timkey, Garcia-Calvo, Houtzager, Nordstrom, Roy, Vaillancourt, Chapman, Nicholson: "A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 29, pp. 17907-11, 1997

Background publications

Wolf, Green: "Suicidal tendencies: apoptotic cell death by caspase family proteinases." in: The Journal of biological chemistry, Vol. 274, Issue 29, pp. 20049-52, 1999

Germain, Affar, DAmours, Dixit, Salvesen, Poirier: "Cleavage of automodified poly(ADP-ribose) polymerase during apoptosis. Evidence for involvement of caspase-7." in: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28379-84, 1999

Chandler, Cohen, MacFarlane: "Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver." in: The Journal of biological chemistry, Vol. 273, Issue 18, pp. 10815-8, 1998

Duan, Orth, Chinnaiyan, Poirier, Froelich, He, Dixit: "ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16720-4, 1996

Images

Product Details anti-Caspase 7 Antibody Target Details Caspase 7 Application Details Handling References for anti-Caspase 7 Antibody (ABIN967332) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (full length) (ABIN967332) Western blot analysis of caspase-7. Lysates from control (lanes 1-3) and camptothecin...
Western Blotting (WB) image for anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (full length) (ABIN967332) Immunoprecipitation/western blot analysis of caspase-7. Lysates from control (lane 1)...