anti-Cow (Bovine) Caspase 8 antibody for Western Blotting

Recommended Caspase 8 Antibody (supplied by: Log in to see )

Antigen
Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8, apoptosis-related cysteine peptidase
  • death related ced-3/Nedd2-like protein
  • caspase-8
  • caspase 8
  • casp8
  • Dredd
  • CASP8
  • AaeL_AAEL014148
  • LOC100222284
  • Casp8
  • LOC100348477
Reactivity
Cow (Bovine), Monkey, Mouse (Murine), Rat (Rattus)
347
119
96
25
21
20
3
2
1
Host
Rabbit
267
111
9
2
Clonality
Polyclonal
Conjugate
This Caspase 8 antibody is un-conjugated
18
13
8
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
346
107
91
74
51
36
35
26
14
13
8
8
6
5
2
2
2
1
1
1
1
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Catalog No. ABIN222878
$ 304.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1873004 ABIN1077918 ICC IHC IP WB Rabbit IgG AA 25-175 Log in to see Polyclonal
3.1873004 ABIN3201520 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 25-175 Log in to see
1 ABIN202629 WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN126965 WB Rabbit Log in to see Polyclonal
1 ABIN2470495 IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1732676 IHC (p) IP WB Rabbit IgG full length Log in to see Polyclonal
1 ABIN241602 WB Rabbit Log in to see Polyclonal
1 ABIN1585723 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2618007 ELISA WB Biotin Rabbit IgG AA 25-175 Log in to see Polyclonal
1 ABIN2618011 ELISA WB FITC Rabbit IgG AA 25-175 Log in to see Polyclonal
1 ABIN1002019 WB Rabbit Log in to see Polyclonal
1 ABIN2219209 IC IHC ELISA WB Rabbit IgG AA 25-175 Log in to see Polyclonal
1 ABIN4965117 WB Rabbit IgG Log in to see Polyclonal
1 ABIN2618016 ELISA WB Mouse IgG AA 25-175 Log in to see
1 ABIN2618002 ELISA WB Rabbit IgG AA 25-175 Log in to see Polyclonal
1 ABIN1173794 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN1173795 ICC IF IHC WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN2899511 ELISA IF/ICC IHC WB Rabbit AA 25-175 Log in to see Polyclonal

Top referenced anti-Caspase 8 antibody for Western Blotting

Similar anti-Caspase 8 Antibodies

Application / Reactivity Mouse (Murine) Cow (Bovine) Monkey Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies
Western Blotting (WB) 21 Antibodies 19 Antibodies 102 Antibodies 78 Antibodies
Immunoprecipitation (IP) 14 Antibodies 3 Antibodies 13 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 3 Antibodies 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 8 Antibodies 7 Antibodies 38 Antibodies 34 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies 5 Antibodies

General

Antigen Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
Reactivity Cow (Bovine), Monkey, Mouse (Murine), Rat (Rattus)
(347), (119), (96), (25), (21), (20), (3), (2), (1)
Host Rabbit
(267), (111), (9), (2)
Clonality
Polyclonal
Conjugate This Caspase 8 antibody is un-conjugated
(18), (13), (8), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(346), (107), (91), (74), (51), (36), (35), (26), (14), (13), (8), (8), (6), (5), (2), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Caspase 8 Antibody

Target Details Caspase 8 Application Details Handling ProductDetails: References for anti-Caspase 8 antibody (ABIN222878) Images
Blocking Peptide Blocking peptide for this product available: ABIN412656
Specificity The immunoaffinity purified antibody detects the full length (55 kDa), intermediate cleavage fragments (26-31 kDa) and cleaved large fragment (18 kDa) of caspase-8.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 392 of mouse caspase-8
Isotype IgG

Target Details Caspase 8

Product Details anti-Caspase 8 Antibody Application Details Handling ProductDetails: References for anti-Caspase 8 antibody (ABIN222878) Images back to top
Antigen
Alternative Name Caspase-8 (CASP8 Antibody Abstract)
Background Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.
Synonyms: CASP8 , MGC78473 , CASP-8, MACH, procaspase-8, MCH5, ALPS2B, FLICE, CAP4, EC 3.4.22.61
Gene ID 12370
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades

Application Details

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Handling ProductDetails: References for anti-Caspase 8 antibody (ABIN222878) Images back to top
Application Notes Western blotting (0.5-4 μg/mL). The immunoaffinity purified antibody detects the full length (55 kDa), intermediate cleavage fragments (26-31 kDa) and cleaved large fragment (18 kDa) of caspase-8.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details ProductDetails: References for anti-Caspase 8 antibody (ABIN222878) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) protein A purified rabbit anti-caspase-8 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

ProductDetails: References for anti-Caspase 8 antibody (ABIN222878)

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling Images back to top
Product cited in:

Mahul-Mellier, Strappazzon, Petiot, Chatellard-Causse, Torch, Blot, Freeman, Kuhn, Garin, Verna, Fraboulet, Sadoul: "Alix and ALG-2 are involved in tumor necrosis factor receptor 1-induced cell death." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34954-65, 2008

Peluffo, Stouffer, Tesone: "Activity and expression of different members of the caspase family in the rat corpus luteum during pregnancy and postpartum." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 5, pp. E1215-23, 2007

Woods, Burns, Bradford, Ross, Kosyk, Swenberg, Cunningham, Rusyn: "WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 98, Issue 2, pp. 366-74, 2007

Peluffo, Bussmann, Stouffer, Tesone: "Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at different stages during the natural estrous cycle." in: Reproduction (Cambridge, England), Vol. 132, Issue 3, pp. 465-75, 2006

Sasaki, Ikeda, Sato, Nakanuma: "Decreased expression of Bmi1 is closely associated with cellular senescence in small bile ducts in primary biliary cirrhosis." in: The American journal of pathology, Vol. 169, Issue 3, pp. 831-45, 2006

Images

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling ProductDetails: References for anti-Caspase 8 antibody (ABIN222878) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (ABIN222878) Western blot analysis of capsase-8 in Jurkat cell treated by camptothecin. Procaspase-8