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|Application / Reactivity||Monkey||Cow (Bovine)||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||11 Antibodies||12 Antibodies||73 Antibodies||51 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies||3 Antibodies||4 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies||4 Antibodies||13 Antibodies||10 Antibodies|
|Immunohistochemistry (IHC)||5 Antibodies||4 Antibodies||26 Antibodies||24 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies||1 Antibodies||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Antigen||Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies|
|Reactivity||Cow (Bovine), Monkey, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Caspase 8 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-Caspase 8 AntibodyTarget Details Caspase 8 Application Details Handling References for anti-Caspase 8 Antibody (ABIN222878) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412656|
|Specificity||The immunoaffinity purified antibody detects the full length (55 kDa), intermediate cleavage fragments (26-31 kDa) and cleaved large fragment (18 kDa) of caspase-8.|
|Immunogen||Synthetic peptide surrounding amino acid 392 of mouse caspase-8|
Target Details Caspase 8Product Details anti-Caspase 8 Antibody Application Details Handling References for anti-Caspase 8 Antibody (ABIN222878) Images back to top
|Alternative Name||Caspase-8 (CASP8 Antibody Abstract)|
Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.
Synonyms: CASP8 , MGC78473 , CASP-8, MACH, procaspase-8, MCH5, ALPS2B, FLICE, CAP4, EC 126.96.36.199
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling|
Application DetailsProduct Details anti-Caspase 8 Antibody Target Details Caspase 8 Handling References for anti-Caspase 8 Antibody (ABIN222878) Images back to top
|Application Notes||Western blotting (0.5-4 μg/mL).|
Country of Animal Origin: China
|Restrictions||For Research Use only|
HandlingProduct Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details References for anti-Caspase 8 Antibody (ABIN222878) Images back to top
|Buffer||100 μg (0.2 mg/mL) protein A purified rabbit anti-caspase-8 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-Caspase 8 Antibody (ABIN222878)Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling Images back to top
|Product cited in:||
Mahul-Mellier, Strappazzon, Petiot et al.: "Alix and ALG-2 are involved in tumor necrosis factor receptor 1-induced cell death." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34954-65, 2008 (PubMed).
Peluffo, Stouffer, Tesone: "Activity and expression of different members of the caspase family in the rat corpus luteum during pregnancy and postpartum." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 5, pp. E1215-23, 2007 (PubMed).
Woods, Burns, Bradford et al.: "WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 98, Issue 2, pp. 366-74, 2007 (PubMed).
Peluffo, Bussmann, Stouffer et al.: "Expression of caspase-2, -3, -8 and -9 proteins and enzyme activity in the corpus luteum of the rat at different stages during the natural estrous cycle." in: Reproduction (Cambridge, England), Vol. 132, Issue 3, pp. 465-75, 2006 (PubMed).
Sasaki, Ikeda, Sato et al.: "Decreased expression of Bmi1 is closely associated with cellular senescence in small bile ducts in primary biliary cirrhosis." in: The American journal of pathology, Vol. 169, Issue 3, pp. 831-45, 2006 (PubMed).
ImagesProduct Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 Antibody (ABIN222878) back to top