anti-Human Caspase 8 antibody for Immunoprecipitation

Recommended Caspase 8 Antibody (supplied by: Log in to see )

Antigen
Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8 L homeolog
  • death related ced-3/Nedd2-like protein
  • caspase 8
  • caspase-8
  • caspase-8-like
  • caspase 8, apoptosis-related cysteine peptidase
  • casp8.L
  • LOC661095
  • CASP8
  • LOC5563818
  • casp8
  • LOC100222284
  • Casp8
Epitope
full length
44
19
18
16
15
12
10
10
9
8
7
6
6
6
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
361
121
96
25
21
20
3
2
1
Host
Mouse
280
114
10
2
Clonality
Monoclonal
Conjugate
This Caspase 8 antibody is un-conjugated
18
13
8
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
361
109
92
79
50
36
36
29
14
13
9
8
6
5
2
2
2
1
1
1
1
Options
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Catalog No. ABIN967335
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967334 IP WB Mouse IgG1, kappa full length Log in to see 3-1 5
1 ABIN252118 ICC IHC (fro) IF IHC (p) IP IHC WB Rabbit Large Subunit, Small Subunit, active Log in to see Polyclonal 33
1 ABIN4288161 IHC IHC (fro) IHC (p) IP WB Rabbit AA 449-464, AA 464-479, AA 481-496, AA 523-538, C-Term Log in to see Polyclonal 3
1 ABIN223019 IP WB Mouse IgG1 N-Term, active Log in to see C502S 5
1 ABIN1168996 ICC IP WB Mouse IgG2b AA 181-478 Log in to see C15 8
1 ABIN957192 IHC (fro) IHC (p) IP WB Rabbit AA 449-464, AA 464-479, AA 481-496, AA 523-538 Log in to see Polyclonal
1 ABIN126934 IP WB Mouse IgG1 Log in to see C502S
1 ABIN957191 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN1077919 ICC IHC IP WB Rabbit IgG AA 217-384 Log in to see Polyclonal
1 ABIN1858254 ICC IHC IP WB Rabbit IgG AA 385-479 Log in to see Polyclonal
1 ABIN1168997 ICC IP WB Mouse IgG2b AA 181-478 Log in to see C15
1 ABIN2962132 ICC IP WB Mouse IgG2b AA 181-478 Log in to see C15
1 ABIN1732676 IHC (p) IP WB Rabbit IgG full length Log in to see Polyclonal
1 ABIN4903139 IF IHC IP WB Mouse IgG Log in to see
1 ABIN1490387 IP WB Sepharose Rabbit Internal Region Log in to see Polyclonal
1 ABIN1490390 IP WB Magnetic Particles Rabbit Internal Region Log in to see Polyclonal
1 ABIN219263 IP ELISA WB Mouse IgG1 C-Term Log in to see
1 ABIN2219227 IC IP WB Mouse IgG2b AA 181-478 Log in to see 6D221
1 ABIN2618021 ICC IP WB Mouse IgG2b AA 181-478 Log in to see C15
1 ABIN1977287 ICC IP WB Mouse IgG2b AA 181-478 Log in to see

General

Antigen Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
Epitope full length
(44), (19), (18), (16), (15), (12), (10), (10), (9), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(361), (121), (96), (25), (21), (20), (3), (2), (1)
Host Mouse
(280), (114), (10), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 8 antibody is un-conjugated
(18), (13), (8), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(361), (109), (92), (79), (50), (36), (36), (29), (14), (13), (9), (8), (6), (5), (2), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Caspase 8 Antibody

Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 antibody (ABIN967335) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Caspase-8 full-length recombinant protein
Clone 3-1
Isotype IgG1, kappa

Target Details Caspase 8

Product Details anti-Caspase 8 Antibody Application Details Handling References for anti-Caspase 8 antibody (ABIN967335) Images back to top
Antigen
Alternative Name Caspase-8 (CASP8 Antibody Abstract)
Background Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transducer, FADD/MORT-1, as well as to other caspase (ICE/Ced-3) cysteine proteases. The N-terminal region of caspase-8 contains an amino acid sequence, termed the death domain, that facilitates caspase-8-FADD direct interaction. FADD therefore acts as an adapter molecule, allowing caspase-8 to become recruited to the cytoplasmic region of Fas following receptor activation. Viral proteins (v-FLIPS) which inhibit recruitment and activation of caspase-8 have been isolated. Caspase-8 is produced as a proenzyme (55/50 kDa doublet) which upon receptor aggregation is proteolytically cleaved into smaller subunits of 40/36 (doublet), and 23 kDa. Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.g., MCF-7) and this phenotype is blocked by overexpression of the caspase-3 protease inhibitor, CrmA. The antibody recognizes both the proform of human caspase-8 (55/50 kDa doublet) as well as the cleaved forms which migrate at 40/36 (doublet) and 23 kDa in SDS/PAGE. It also immunoprecipitates the full-length human caspase-8. Full-length recombinant human caspase-8 protein was used as immunogen.
Synonyms: FLICE, MACH-1, Mch5
Molecular Weight 55/50 kDa, 40/36 kDa, 23 kDa
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades

Application Details

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Handling References for anti-Caspase 8 antibody (ABIN967335) Images back to top
Application Notes Applications include western blot analysis (0.125 - 0.5 µg/ml) and immunoprecipitation (4 µg/200 µg cell lysate). Jurkat T cells (ATCC CRL-1573) are suggested as a positive control. Antibodies-online offers several caspase-8 antibodies. A Jurkat model cell system was used to evaluate these antibodies, these results are summarized in the following table. However, actual bands observed could vary according to the cell model system or treatment used.
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details References for anti-Caspase 8 antibody (ABIN967335) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-Caspase 8 antibody (ABIN967335)

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling Images back to top
Product cited in:

Boesen-de Cock, Tepper, de Vries, van Blitterswijk, Borst: "Common regulation of apoptosis signaling induced by CD95 and the DNA-damaging stimuli etoposide and gamma-radiation downstream from caspase-8 activation." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14255-61, 1999

Thome, Martinon, Hofmann, Rubio, Steiner, Schneider, Mattmann, Tschopp: "Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-kappaB transcription factor and c-Jun N-terminal kinase." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 9962-8, 1999

Cock, Tepper, de Vries, van Blitterswijk, Borst: "CD95 (Fas/APO-1) induces ceramide formation and apoptosis in the absence of a functional acid sphingomyelinase." in: The Journal of biological chemistry, Vol. 273, Issue 13, pp. 7560-5, 1998

Thome, Schneider, Hofmann, Fickenscher, Meinl, Neipel, Mattmann, Burns, Bodmer, Schröter, Scaffidi, Krammer, Peter, Tschopp: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997

Muzio, Chinnaiyan, Kischkel, ORourke, Shevchenko, Ni, Scaffidi, Bretz, Zhang, Gentz, Mann, Krammer, Peter, Dixit: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996

Images

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 antibody (ABIN967335) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (full length) antibody (ABIN967335) Western blot analysis of caspase-8. Lysates from control (lanes 1-3) and camptothecin...
Western Blotting (WB) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (full length) antibody (ABIN967335) Immunoprecipitation/western blot analysis of caspase-8. Lysates from control (lane 1)...
Western Blotting (WB) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (full length) antibody (ABIN967335) anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (full length) antibody (Image 3)