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anti-Human Caspase 8 antibody for Immunoprecipitation

Recommended Caspase 8 Antibody (supplied by: Log in to see )

Antigen
Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
  • ALPS2B
  • CAP4
  • Casp-8
  • CASP-8
  • casp8
  • CASP8
  • casp8-A
  • caspase-8
  • FLICE
  • MACH
  • Mch5
  • MCH5
  • xCaspase-8
  • zgc:92075
Epitope
full length
48
18
17
17
13
10
10
8
8
7
7
6
6
5
5
5
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
339
96
69
18
18
14
4
1
1
1
Host
Mouse
272
100
11
2
Clonality
Monoclonal
Conjugate
This Caspase 8 antibody is un-conjugated
17
12
8
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
323
113
81
70
41
34
31
24
12
11
8
5
2
2
1
1
1
1
1
1
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Catalog No. ABIN967335
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089465064 ABIN967334 IP WB Mouse IgG1, kappa full length Log in to see 3-1 5
0.00089465064 ABIN223019 IP WB Mouse IgG1 N-Term, active Log in to see C502S 5
0.00089465064 ABIN1168996 ICC IP WB Mouse IgG2b AA 181-478 Log in to see C15 8
0.00089465064 ABIN180429 IP WB Mouse IgG1 Log in to see 8CSP03 2
0.00089465064 ABIN189211 FACS IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see E6
0.00089465064 ABIN372513 IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
0.00089465064 ABIN269525 IHC (p) IP IHC WB Mouse IgG1 kappa Log in to see 8CSP03
0.00089465064 ABIN957192 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00089465064 ABIN126934 IP WB Mouse IgG1 Log in to see C502S
0.00089465064 ABIN957191 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00089465064 ABIN219263 IP ELISA WB Mouse IgG1 C-Term Log in to see
0.00089465064 ABIN1168997 ICC IP WB Mouse IgG2b AA 181-478 Log in to see C15
0.00089465064 ABIN307184 IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
0.00089465064 ABIN241741 IP WB Mouse IgG1 N-Term Log in to see
0.00089465064 ABIN210738 IP WB Rabbit IgG Log in to see Polyclonal
0.00089465064 ABIN2470495 IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089465064 ABIN1490390 IP Rabbit Internal Region Log in to see Polyclonal
0.00089465064 ABIN1490387 IP Rabbit Internal Region Log in to see Polyclonal
0.00089465064 ABIN2962132 ICC IP WB Mouse IgG2b AA 181-478 Log in to see C15
0.00089465064 ABIN219257 IP WB Mouse IgG1 Log in to see

Top referenced anti-Caspase 8 antibody for Immunoprecipitation

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General

Antigen Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
Epitope full length
(48), (18), (17), (17), (13), (10), (10), (8), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(339), (96), (69), (18), (18), (14), (4), (1), (1), (1)
Host Mouse
(272), (100), (11), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 8 antibody is un-conjugated
(17), (12), (8), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(323), (113), (81), (70), (41), (34), (31), (24), (12), (11), (8), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Caspase 8 Antibody

Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 Antibody (ABIN967335) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Caspase-8 full-length recombinant protein
Clone 3-1
Isotype IgG1, kappa

Target Details Caspase 8

Product Details anti-Caspase 8 Antibody Application Details Handling References for anti-Caspase 8 Antibody (ABIN967335) Images back to top
Antigen
Alternative Name Caspase-8 (CASP8 Antibody Abstract)
Background Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transducer, FADD/MORT-1, as well as to other caspase (ICE/Ced-3) cysteine proteases. The N-terminal region of caspase-8 contains an amino acid sequence, termed the death domain, that facilitates caspase-8-FADD direct interaction. FADD therefore acts as an adapter molecule, allowing caspase-8 to become recruited to the cytoplasmic region of Fas following receptor activation. Viral proteins (v-FLIPS) which inhibit recruitment and activation of caspase-8 have been isolated. Caspase-8 is produced as a proenzyme (55/50 kDa doublet) which upon receptor aggregation is proteolytically cleaved into smaller subunits of 40/36 (doublet), and 23 kDa. Overexpression of caspase-8 is sufficient to induce apoptosis in certain cell lines (e.g., MCF-7) and this phenotype is blocked by overexpression of the caspase-3 protease inhibitor, CrmA. The antibody recognizes both the proform of human caspase-8 (55/50 kDa doublet) as well as the cleaved forms which migrate at 40/36 (doublet) and 23 kDa in SDS/PAGE. It also immunoprecipitates the full-length human caspase-8. Full-length recombinant human caspase-8 protein was used as immunogen.
Synonyms: FLICE, MACH-1, Mch5
Molecular Weight 55/50 kDa, 40/36 kDa, 23 kDa
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling

Application Details

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Handling References for anti-Caspase 8 Antibody (ABIN967335) Images back to top
Application Notes Applications include western blot analysis (0.125 - 0.5 µg/ml) and immunoprecipitation (4 µg/200 µg cell lysate). Jurkat T cells (ATCC CRL-1573) are suggested as a positive control. Antibodies-online offers several caspase-8 antibodies. A Jurkat model cell system was used to evaluate these antibodies, these results are summarized in the following table. However, actual bands observed could vary according to the cell model system or treatment used.
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details References for anti-Caspase 8 Antibody (ABIN967335) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-Caspase 8 Antibody (ABIN967335)

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling Images back to top
Product cited in:

Boesen-de Cock, Tepper, de Vries, van Blitterswijk, Borst: "Common regulation of apoptosis signaling induced by CD95 and the DNA-damaging stimuli etoposide and gamma-radiation downstream from caspase-8 activation." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14255-61, 1999

Thome, Martinon, Hofmann, Rubio, Steiner, Schneider, Mattmann, Tschopp: "Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-kappaB transcription factor and c-Jun N-terminal kinase." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 9962-8, 1999

Cock, Tepper, de Vries, van Blitterswijk, Borst: "CD95 (Fas/APO-1) induces ceramide formation and apoptosis in the absence of a functional acid sphingomyelinase." in: The Journal of biological chemistry, Vol. 273, Issue 13, pp. 7560-5, 1998

Thome, Schneider, Hofmann, Fickenscher, Meinl, Neipel, Mattmann, Burns, Bodmer, Schröter, Scaffidi, Krammer, Peter, Tschopp: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997

Muzio, Chinnaiyan, Kischkel, ORourke, Shevchenko, Ni, Scaffidi, Bretz, Zhang, Gentz, Mann, Krammer, Peter, Dixit: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996

Images

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 Antibody (ABIN967335) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (full length) (ABIN967335) Western blot analysis of caspase-8. Lysates from control (lanes 1-3) and camptothecin...
Western Blotting (WB) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (full length) (ABIN967335) Immunoprecipitation/western blot analysis of caspase-8. Lysates from control (lane 1)...
Western Blotting (WB) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (full length) (ABIN967335) anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (full length) antibody (Image 3)