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anti-Mouse (Murine) Caspase 8 antibody for Flow Cytometry

Recommended Caspase 8 Antibody (supplied by: Log in to see )

Antigen
Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8, apoptosis-related cysteine peptidase
  • death related ced-3/Nedd2-like protein
  • caspase-8
  • caspase 8
  • casp8
  • Dredd
  • CASP8
  • AaeL_AAEL014148
  • LOC100222284
  • Casp8
  • LOC100348477
Reactivity
Human, Mouse (Murine), Monkey, Rat (Rattus)
350
123
98
25
22
20
3
2
1
Host
Mouse
273
108
10
2
Clonality
Monoclonal
Conjugate
This Caspase 8 antibody is un-conjugated
18
13
8
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
350
113
93
75
50
36
36
29
14
13
8
8
6
5
2
2
2
1
1
1
1
1
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Catalog No. ABIN969003
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.407578 ABIN2745526 ICC FACS IHC ELISA WB Rat IgG1 Log in to see 3B10
13.407578 ABIN2745525 FACS IHC ELISA WB Rat IgG1 Log in to see 1G12
13.407578 ABIN4288173 ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 1H11
13.407578 ABIN1105678 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 1H11
13.407578 ABIN1846137 FACS IHC ELISA WB Mouse IgG1 Log in to see
1 ABIN207902 FACS WB Mouse IgG1 AA 217-222 Log in to see 90A992
1 ABIN1497105 ELISA FACS IHC (p) WB Mouse IgG1 Log in to see 1H11
1 ABIN4880223 ELISA FACS IHC WB Mouse IgG1 Log in to see 1H11
1 ABIN2618019 ICC FACS IHC ELISA WB Rat IgG1, kappa Log in to see 3B10
1 ABIN2618020 ICC FACS ELISA WB Rat IgG1, kappa Log in to see 1G12
1 ABIN4901141 ELISA FACS IHC WB Mouse IgG1 Log in to see 1H11

Top referenced anti-Caspase 8 antibody for Flow Cytometry

Similar anti-Caspase 8 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 20 Antibodies 36 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Flow Cytometry (FACS) 7 Antibodies 12 Antibodies
Immunocytochemistry (ICC) 12 Antibodies 16 Antibodies
Immunofluorescence (IF) 9 Antibodies 9 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Western Blotting (WB) 80 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies

General

Antigen Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) Antibodies
Reactivity Human, Mouse (Murine), Monkey, Rat (Rattus)
(350), (123), (98), (25), (22), (20), (3), (2), (1)
Host Mouse
(273), (108), (10), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 8 antibody is un-conjugated
(18), (13), (8), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(350), (113), (93), (75), (50), (36), (36), (29), (14), (13), (8), (8), (6), (5), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Caspase 8 Antibody

Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 Antibody (ABIN969003) Images
Purification Ascites
Immunogen Purified recombinant fragment of human CASP8 expressed in E. coli.
Clone 1H11
Isotype IgG1

Target Details Caspase 8

Product Details anti-Caspase 8 Antibody Application Details Handling References for anti-Caspase 8 Antibody (ABIN969003) Images back to top
Antigen
Alternative Name CASP8 (CASP8 Antibody Abstract)
Background This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Molecular Weight 26 kDa
Gene ID 841
HGNC 841
Pathways Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response

Application Details

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Handling References for anti-Caspase 8 Antibody (ABIN969003) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details References for anti-Caspase 8 Antibody (ABIN969003) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-Caspase 8 Antibody (ABIN969003)

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling Images back to top
Product cited in:

Fulda: "Caspase-8 in cancer biology and therapy." in: Cancer letters, Vol. 281, Issue 2, pp. 128-33, 2009

Jani, Gobejishvili, Hote, Barve, Joshi-Barve, Kharebava, Suttles, Chen, McClain, Barve: "Inhibition of methionine adenosyltransferase II induces FasL expression, Fas-DISC formation and caspase-8-dependent apoptotic death in T leukemic cells." in: Cell research, Vol. 19, Issue 3, pp. 358-69, 2009

Images

Product Details anti-Caspase 8 Antibody Target Details Caspase 8 Application Details Handling References for anti-Caspase 8 Antibody (ABIN969003) back to top
Supplier Images
ELISA image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (ABIN969003) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (ABIN969003) Figure 2: Flow cytometric analysis of NIH/3T3 cells using CASP8 mouse mAb (blue) and ...
Immunohistochemistry (IHC) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (ABIN969003) Figure 3: Immunohistochemical analysis of paraffin-embedded colon cancer tissues usin...
Immunohistochemistry (IHC) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (ABIN969003) Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues usin...
Western Blotting (WB) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (ABIN969003) Figure 5: Western blot analysis using CASP8 mouse mAb against Hela (1), Jurkat (2), T...