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anti-Monkey CD11b antibody for Flow Cytometry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Human, Monkey
634
485
196
66
25
20
10
9
9
8
8
5
4
3
2
2
2
1
1
Host
Mouse
489
395
154
17
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
166
128
93
51
16
15
15
14
14
13
11
11
9
9
7
6
6
6
6
5
5
5
5
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
823
248
221
162
157
123
98
80
75
70
24
19
17
13
8
5
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN118517
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.28290245 ABIN118513 FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
0.0010564557 ABIN118516 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 5
0.0010564557 ABIN118515 FACS FITC Mouse IgG1 Log in to see ICRF44 4
0.0010564557 ABIN118518 FACS PE Mouse IgG1 Log in to see ICRF44 4
0.0010564557 ABIN319675 FACS FITC Mouse IgG1 Log in to see ICRF44 4
0.0010564557 ABIN319676 FACS IHC (fro) Mouse IgG1 Log in to see ICRF44 4
0.0010564557 ABIN319677 FACS PE Mouse IgG1 Log in to see ICRF44 4
0.0010564557 ABIN3199418 FACS Func IHC (fro) IP APC Rat IgG2b Log in to see M1-70
0.0010564557 ABIN3200842 FACS Func IHC (fro) IP FITC Rat IgG2b Log in to see M1-70
0.0010564557 ABIN3200843 FACS Func IHC (fro) IP PE Rat IgG2b Log in to see M1-70
0.0010564557 ABIN3199416 FACS Func ICC IHC (fro) IP Biotin Mouse IgG1 Log in to see ICRF44
0.0010564557 ABIN3200840 FACS Func ICC IHC (fro) IP FITC Mouse IgG1 Log in to see ICRF44
0.0010564557 ABIN3200841 FACS Func ICC IHC (fro) IP PE Mouse IgG1 Log in to see ICRF44
0.0010564557 ABIN3199414 FACS Func ICC IHC (fro) IP Mouse IgG1 Log in to see ICRF44
0.0010564557 ABIN3199417 FACS Func IHC (fro) IP Rat IgG2b Log in to see M1-70
0.0010564557 ABIN3199415 FACS Func ICC IHC (fro) IP Mouse IgG1 Log in to see ICRF44
0.0010564557 ABIN3036610 Func ICC FACS IF IP IHC Mouse IgG1 Log in to see
0.0010564557 ABIN3036618 Func FACS IP IHC APC Rat IgG2b Log in to see
0.0010564557 ABIN3036620 Func ICC FACS IF IP IHC PE Mouse IgG1 Log in to see
0.0010564557 ABIN3036617 Func FACS IP IHC PE Rat IgG2b Log in to see

Top referenced anti-CD11b antibody for Flow Cytometry

Similar anti-CD11b Antibodies

Application / Reactivity Monkey Human
Flow Cytometry (FACS) 26 Antibodies 465 Antibodies
Functional Studies (Func) 20 Antibodies 61 Antibodies
Immunocytochemistry (ICC) 10 Antibodies 66 Antibodies
Immunofluorescence (IF) 5 Antibodies 99 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 13 Antibodies
Immunoprecipitation (IP) 21 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 11 Antibodies
ELISA 64 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Monkey
(634), (485), (196), (66), (25), (20), (10), (9), (9), (8), (8), (5), (4), (3), (2), (2), (2), (1), (1)
Host Mouse
(489), (395), (154), (17), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(166), (128), (93), (51), (16), (15), (15), (14), (14), (13), (11), (11), (9), (9), (7), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(823), (248), (221), (162), (157), (123), (98), (80), (75), (70), (24), (19), (17), (13), (8), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN118517) Images
Specificity This antibody recognises the CD11b cell surface glycoprotein. This antibody blocks some functions of CD11b, although full definition is still to be completed.
Purification Affinity Chromatography on Protein G.
Immunogen Rheumatoid synovial cells and human mocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Clone ICRF44
Isotype IgG1

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN118517) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b is a 165kD molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 3684
NCBI Accession NP_000623
UniProt P11215
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN118517) Images back to top
Application Notes Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN118517) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD11b Antibody (ABIN118517)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Sotiriou, Orlova, Al-Fakhri, Ihanus, Economopoulou, Isermann, Bdeir, Nawroth, Preissner, Gahmberg, Koschinsky, Chavakis: "Lipoprotein(a) in atherosclerotic plaques recruits inflammatory cells through interaction with Mac-1 integrin." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 559-61, 2006

Background publications

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Dransfield, Cabañas, Barrett, Hogg: "Interaction of leukocyte integrins with ligand is necessary but not sufficient for function." in: The Journal of cell biology, Vol. 116, Issue 6, pp. 1527-35, 1992

Malhotra, Hogg, Sim: "Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3)." in: European journal of immunology, Vol. 16, Issue 9, pp. 1117-23, 1986

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN118517) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD11b antibody (Integrin alpha M) (ABIN118517) Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD11b