anti-Monkey CD11b antibody for Flow Cytometry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Reactivity
Human, Monkey
691
474
192
78
30
19
17
15
13
11
9
9
7
7
5
4
4
4
2
2
Host
Mouse
494
371
164
16
5
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
147
119
88
48
20
19
15
15
14
14
13
11
10
9
9
9
8
8
7
7
7
6
6
6
6
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
843
234
228
172
168
139
96
95
94
66
25
21
21
13
8
6
5
5
4
3
3
1
1
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN118517
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.326658 ABIN118513 FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
1 ABIN118516 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 5
1 ABIN118515 FACS FITC Mouse IgG1 Log in to see ICRF44 4
1 ABIN118518 FACS PE Mouse IgG1 Log in to see ICRF44 4
1 ABIN319675 FACS FITC Mouse IgG1 Log in to see ICRF44 4
1 ABIN319677 FACS PE Mouse IgG1 Log in to see ICRF44 4
1 ABIN319676 FACS IHC (fro) Mouse IgG1 Log in to see ICRF44 4
1 ABIN3200842 FACS Func IHC (fro) IP FITC Rat IgG2b Log in to see M1-70
1 ABIN205315 FACS IHC (fro) Mouse IgG1 Log in to see ICRF44
1 ABIN3199416 FACS Func ICC IHC (fro) IP Biotin Mouse IgG1 Log in to see ICRF44
1 ABIN3200841 FACS Func ICC IHC (fro) IP PE Mouse IgG1 Log in to see ICRF44
1 ABIN3199414 FACS Func ICC IHC (fro) IP Mouse IgG1 Log in to see ICRF44
1 ABIN3199418 FACS Func IHC (fro) IP APC Rat IgG2b Log in to see M1-70
1 ABIN3200843 FACS Func IHC (fro) IP PE Rat IgG2b Log in to see M1-70
1 ABIN3200840 FACS Func ICC IHC (fro) IP FITC Mouse IgG1 Log in to see ICRF44
1 ABIN600698 IHC (fro) FACS IP Mouse IgG1 Log in to see
1 ABIN3199415 FACS Func ICC IHC (fro) IP Mouse IgG1 Log in to see ICRF44
1 ABIN600699 IHC (fro) FACS IHC (p) IP Mouse IgG1 Log in to see
1 ABIN3199417 FACS Func IHC (fro) IP Rat IgG2b Log in to see M1-70
1 ABIN3036610 FACS IF/ICC IHC IP Mouse IgG1 Log in to see

Top referenced anti-CD11b antibody for Flow Cytometry

  • Human Monoclonal CD11b Primary Antibody for FACS - ABIN319675 (4 Publications): Sotiriou, Orlova, Al-Fakhri, Ihanus, Economopoulou, Isermann, Bdeir, Nawroth, Preissner, Gahmberg, Koschinsky, Chavakis: Lipoprotein(a) in atherosclerotic plaques recruits inflammatory cells through interaction with Mac-1 integrin. in FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 (PubMed)

  • Human Monoclonal CD11b Primary Antibody for FACS - ABIN319677 (4 Publications): Yoshino, Ami, Terao, Tashiro, Honda: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. in Experimental animals / Japanese Association for Laboratory Animal Science 2000 (PubMed)

  • Human Monoclonal CD11b Primary Antibody for FACS, IHC (fro) - ABIN319676 (4 Publications): Dransfield, Cabañas, Barrett, Hogg: Interaction of leukocyte integrins with ligand is necessary but not sufficient for function. in The Journal of cell biology 1992 (PubMed)

  • Human Monoclonal CD11b Primary Antibody for FACS, IHC (fro) - ABIN118513 (4 Publications): Malhotra, Hogg, Sim: Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3). in European journal of immunology 1986 (PubMed)

  • Human Monoclonal CD11b Primary Antibody for Func, FACS - ABIN118516 (5 Publications): Ramacciotti, Blackburn, Hawley, Vandy, Ballard-Lipka, Stabler, Baker, Guire, Rectenwald, Henke, Myers, Wakefield: Evaluation of soluble P-selectin as a marker for the diagnosis of deep venous thrombosis. in Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis 2011 (PubMed)

Similar anti-CD11b Antibodies

Application / Reactivity Monkey Human
ELISA 1 Antibodies 77 Antibodies
Flow Cytometry (FACS) 29 Antibodies 541 Antibodies
Functional Studies (Func) 15 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Immunoprecipitation (IP) 23 Antibodies
Western Blotting (WB) 1 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 17 Antibodies
Cytometry by Time of Flight (CyTOF) 6 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Monkey
(691), (474), (192), (78), (30), (19), (17), (15), (13), (11), (9), (9), (7), (7), (5), (4), (4), (4), (2), (2)
Host Mouse
(494), (371), (164), (16), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(147), (119), (88), (48), (20), (19), (15), (15), (14), (14), (13), (11), (10), (9), (9), (9), (8), (8), (7), (7), (7), (6), (6), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(843), (234), (228), (172), (168), (139), (96), (95), (94), (66), (25), (21), (21), (13), (8), (6), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling ProductDetails: References for anti-CD11b antibody (ABIN118517) Images
Specificity This antibody recognises the CD11b cell surface glycoprotein. This antibody blocks some functions of CD11b, although full definition is still to be completed.
Purification Affinity Chromatography on Protein G.
Immunogen Rheumatoid synovial cells and human mocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Clone ICRF44
Isotype IgG1

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling ProductDetails: References for anti-CD11b antibody (ABIN118517) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b is a 165kD molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 3684
NCBI Accession NP_000623
UniProt P11215
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling ProductDetails: References for anti-CD11b antibody (ABIN118517) Images back to top
Application Notes Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells or 100 μL whole blood. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details ProductDetails: References for anti-CD11b antibody (ABIN118517) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD11b antibody (ABIN118517)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Sotiriou, Orlova, Al-Fakhri, Ihanus, Economopoulou, Isermann, Bdeir, Nawroth, Preissner, Gahmberg, Koschinsky, Chavakis: "Lipoprotein(a) in atherosclerotic plaques recruits inflammatory cells through interaction with Mac-1 integrin." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 559-61, 2006

Background publications

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Dransfield, Cabañas, Barrett, Hogg: "Interaction of leukocyte integrins with ligand is necessary but not sufficient for function." in: The Journal of cell biology, Vol. 116, Issue 6, pp. 1527-35, 1992

Malhotra, Hogg, Sim: "Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3)." in: European journal of immunology, Vol. 16, Issue 9, pp. 1117-23, 1986

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling ProductDetails: References for anti-CD11b antibody (ABIN118517) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD11b antibody (Integrin alpha M) (ABIN118517) Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD11b