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anti-Monkey CD11b antibody for Functional Studies

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Human, Monkey
615
467
146
67
25
20
17
17
17
11
10
9
5
4
3
2
2
1
Host
Mouse
476
413
117
17
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
164
123
88
51
18
17
12
12
11
11
11
10
10
9
7
6
5
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
799
243
207
161
140
112
78
77
71
69
19
15
11
7
6
5
4
3
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN118516
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008771965 ABIN118517 Func FACS IHC (fro) IP Mouse IgG1 Log in to see ICRF44 4
0.0008771965 ABIN3200842 FACS Func IHC (fro) IP FITC Rat IgG2b Log in to see M1-70
0.0008771965 ABIN3200840 FACS Func ICC IHC (fro) IP FITC Mouse IgG1 Log in to see ICRF44
0.0008771965 ABIN3200843 FACS Func IHC (fro) IP PE Rat IgG2b Log in to see M1-70
0.0008771965 ABIN3200841 FACS Func ICC IHC (fro) IP PE Mouse IgG1 Log in to see ICRF44
0.0008771965 ABIN3199416 FACS Func ICC IHC (fro) IP Biotin Mouse IgG1 Log in to see ICRF44
0.0008771965 ABIN3199418 FACS Func IHC (fro) IP APC Rat IgG2b Log in to see M1-70
0.0008771965 ABIN3199414 FACS Func ICC IHC (fro) IP Mouse IgG1 Log in to see ICRF44
0.0008771965 ABIN3199415 FACS Func ICC IHC (fro) IP Mouse IgG1 Log in to see ICRF44
0.0008771965 ABIN3199417 FACS Func IHC (fro) IP Rat IgG2b Log in to see M1-70
0.0008771965 ABIN3036610 Func ICC FACS IF IP IHC Mouse IgG1 Log in to see
0.0008771965 ABIN3036617 Func FACS IP IHC PE Rat IgG2b Log in to see
0.0008771965 ABIN3036618 Func FACS IP IHC APC Rat IgG2b Log in to see
0.0008771965 ABIN3036619 Func FACS IP IHC FITC Rat IgG2b Log in to see
0.0008771965 ABIN3036620 Func ICC FACS IF IP IHC PE Mouse IgG1 Log in to see
0.0008771965 ABIN3036621 Func ICC FACS IF IP IHC FITC Mouse IgG1 Log in to see
0.0008771965 ABIN3036622 Func ICC FACS IF IP IHC Biotin Mouse IgG1 Log in to see
0.0008771965 ABIN3036609 Func ICC FACS IF IP IHC Mouse IgG1 Log in to see
0.0008771965 ABIN3036611 Func FACS IP IHC Rat IgG2b Log in to see

Top referenced anti-CD11b antibody for Functional Studies

Similar anti-CD11b Antibodies

Application / Reactivity Monkey Human
Flow Cytometry (FACS) 26 Antibodies 446 Antibodies
Functional Studies (Func) 20 Antibodies 60 Antibodies
Immunocytochemistry (ICC) 10 Antibodies 60 Antibodies
Immunofluorescence (IF) 5 Antibodies 92 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 13 Antibodies
Immunoprecipitation (IP) 21 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 11 Antibodies
ELISA 62 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Monkey
(615), (467), (146), (67), (25), (20), (17), (17), (17), (11), (10), (9), (5), (4), (3), (2), (2), (1)
Host Mouse
(476), (413), (117), (17), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(164), (123), (88), (51), (18), (17), (12), (12), (11), (11), (11), (10), (10), (9), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(799), (243), (207), (161), (140), (112), (78), (77), (71), (69), (19), (15), (11), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN118516) Images
Specificity This antibody recognizes the CD11b cell surface glycoprotein. Clone ICRF44 has been reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release.
No Cross-Reactivity Cat (Feline)
Cross-Reactivity (Details) Species reactivity (tested):Human, Cynomolgus Monkey , Rhesus Monkey, Baboon.
Purification Affinity Chromatography on Protein G
Immunogen Rheumatoid synovial cells and Human monocytes. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Clone ICRF44
Isotype IgG1

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN118516) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b is a 165 kD molecule also know as the alpha-M integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta-2 integrin and is found on monocytes, granulocytes, NK cells and some peripheral blood lymphocytes. ICR44 significantly blocks homotypic PNM aggregation on response to FMLP (3,4).Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 3684
NCBI Accession NP_000623
UniProt P11215
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN118516) Images back to top
Application Notes Flow Cytometry (use 10 μL of 1:10 - 1:100 diluted antibody to label 10^6 cells or 100 μL whole blood). Immunoprecipitation Immunohistochemistry on frozen sections (1:100 - 1:1000) Recommended positive control tissue: Human tonsil. Not suitable for paraffin embedded sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN118516) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the reconstituted antibody at 2-8 °C for one month (add 0.09% Sodium Azide) or at -20 °C for longer.

References for anti-CD11b Antibody (ABIN118516)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Ramacciotti, Blackburn, Hawley, Vandy, Ballard-Lipka, Stabler, Baker, Guire, Rectenwald, Henke, Myers, Wakefield: "Evaluation of soluble P-selectin as a marker for the diagnosis of deep venous thrombosis." in: Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, Vol. 17, Issue 4, pp. 425-31, 2011

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Dransfield, Cabañas, Barrett, Hogg: "Interaction of leukocyte integrins with ligand is necessary but not sufficient for function." in: The Journal of cell biology, Vol. 116, Issue 6, pp. 1527-35, 1992

Malhotra, Hogg, Sim: "Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3)." in: European journal of immunology, Vol. 16, Issue 9, pp. 1117-23, 1986

Product cited in:

Sotiriou, Orlova, Al-Fakhri, Ihanus, Economopoulou, Isermann, Bdeir, Nawroth, Preissner, Gahmberg, Koschinsky, Chavakis: "Lipoprotein(a) in atherosclerotic plaques recruits inflammatory cells through interaction with Mac-1 integrin." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 559-61, 2006

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN118516) back to top
Supplier Images
 image for anti-CD11b antibody (Integrin alpha M) (ABIN118516) Staining of human peripheral blood granulocytes with Mouse anti Human CD11b (ABIN1185...