|Application / Reactivity||Human||Mouse (Murine)|
|Western Blotting (WB)||93 Antibodies||41 Antibodies|
|Staining Methods (StM)||2 Antibodies||1 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Multiplex Assay (MA)||1 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||6 Antibodies||4 Antibodies|
|In vitro Assay (in vitro)||1 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||101 Antibodies||108 Antibodies|
|Immunohistochemistry (Paraformaldehyde) (IHC (pfa))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies||36 Antibodies|
|Immunohistochemistry (IHC)||97 Antibodies||85 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||53 Antibodies|
|Antigen||Integrin alpha M (ITGAM) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This CD11b antibody is conjugated to Alexa Fluor 700 Alternatives|
Biochemical Assay (BCA)
|12 references available|
|Supplier||Log in to see|
Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN2658026) Images
|Cross-Reactivity||Cynomolgus, Rhesus Monkey, Baboon, Chimpanzee, Rabbit|
|Purification||The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions.|
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN2658026) Images back to top
|Alternative Name||CD11b (ITGAM Antibody Abstract)|
|Background||CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.|
|Research Area||Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN2658026) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN2658026) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C|
References for anti-CD11b Antibody (ABIN2658026)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Kim, Nakano, Das et al.: "Flow-induced protein kinase A-CREB pathway acts via BMP signaling to promote HSC emergence." in: The Journal of experimental medicine, Vol. 212, Issue 5, pp. 633-48, 2015 (PubMed).
DAmico, Wu: "The early progenitors of mouse dendritic cells and plasmacytoid predendritic cells are within the bone marrow hemopoietic precursors expressing Flt3." in: The Journal of experimental medicine, Vol. 198, Issue 2, pp. 293-303, 2003 (PubMed).
Brickson, Ji, Schell et al.: "M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 95, Issue 3, pp. 969-76, 2003 (PubMed).
Coxon, Rieu, Barkalow et al.: "A novel role for the beta 2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation." in: Immunity, Vol. 5, Issue 6, pp. 653-66, 1997 (PubMed).
Allen, Aderem: "Molecular definition of distinct cytoskeletal structures involved in complement- and Fc receptor-mediated phagocytosis in macrophages." in: The Journal of experimental medicine, Vol. 184, Issue 2, pp. 627-37, 1996 (PubMed).
Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).
Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, 1981 (PubMed).
Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983 (PubMed).
Ho, Springer: "Biosynthesis and assembly of the alpha and beta subunits of Mac-1, a macrophage glycoprotein associated with complement receptor function." in: The Journal of biological chemistry, Vol. 258, Issue 5, pp. 2766-9, 1983 (PubMed).
Springer, Davignon, Ho et al.: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, 1983 (PubMed).
Noel, Mosser, Edelson: "Role of complement in mouse macrophage binding of Haemophilus influenzae type b." in: The Journal of clinical investigation, Vol. 85, Issue 1, pp. 208-18, 1990 (PubMed).
Springer, Galfrè, Secher et al.: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).
ImagesProduct Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN2658026) back to top