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anti-Mouse (Murine) CD11b antibody for Immunocytochemistry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Epitope
Internal Region, AA 250-350
29
19
15
14
14
8
6
5
5
5
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
612
461
142
67
26
20
17
17
17
11
10
9
5
4
3
2
2
1
Host
Rabbit
477
411
113
17
4
2
Clonality
Polyclonal
Conjugate
This CD11b antibody is un-conjugated
163
123
87
51
18
17
12
12
11
11
11
10
9
9
7
6
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
798
243
205
160
136
111
78
75
72
67
19
15
11
7
6
5
4
3
3
3
3
3
2
1
1
1
Supplier
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Catalog No. ABIN409931
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009956994 ABIN1732800 FACS ICC IF IHC (p) WB Rabbit Internal Region, AA 250-350 Log in to see Polyclonal
0.0009956994 ABIN1859497 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 808-931 Log in to see Polyclonal
0.0009956994 ABIN1859495 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 248-430 Log in to see Polyclonal
0.0009956994 ABIN226327 ICC FITC Rat IgG2b Log in to see
0.0009956994 ABIN4289434 FACS ICC IF IHC IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289435 FACS ICC IF IHC IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289470 FACS ICC IF IHC IHC (fro) IHC (p) IP PE Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289447 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 405 Rabbit AA 250-350, Internal Region Log in to see Polyclonal
0.0009956994 ABIN4289448 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit AA 250-350, Internal Region Log in to see Polyclonal
0.0009956994 ABIN4289449 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 647 Rabbit AA 250-350, Internal Region Log in to see Polyclonal
0.0009956994 ABIN4289450 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 700 Rabbit AA 250-350, Internal Region Log in to see Polyclonal
0.0009956994 ABIN4289458 FACS ICC IF IHC IHC (fro) IHC (p) IP Alexa Fluor 405 Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289459 FACS ICC IF IHC IHC (fro) IHC (p) IP Alexa Fluor 488 Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289460 FACS ICC IF IHC IHC (fro) IHC (p) IP Alexa Fluor 647 Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289461 FACS ICC IF IHC IHC (fro) IHC (p) IP Alexa Fluor 700 Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289462 FACS ICC IF IHC IHC (fro) IHC (p) IP Allophycocyanin Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289463 FACS ICC IF IHC IHC (fro) IHC (p) IP Biotin Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289464 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 650 Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289465 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 680 Rat IgG2b Log in to see M1-70-15
0.0009956994 ABIN4289466 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 488 Rat IgG2b Log in to see M1-70-15

Similar anti-CD11b Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies 8 Antibodies
ELISA 13 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 87 Antibodies
Functional Studies (Func) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 33 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 28 Antibodies
Western Blotting (WB) 36 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Epitope Internal Region, AA 250-350
(29), (19), (15), (14), (14), (8), (6), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(612), (461), (142), (67), (26), (20), (17), (17), (17), (11), (10), (9), (5), (4), (3), (2), (2), (1)
Host Rabbit
(477), (411), (113), (17), (4), (2)
Clonality
Polyclonal
Conjugate This CD11b antibody is un-conjugated
(163), (123), (87), (51), (18), (17), (12), (12), (11), (11), (11), (10), (9), (9), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(798), (243), (205), (160), (136), (111), (78), (75), (72), (67), (19), (15), (11), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN409931) Images
Cross-Reactivity (Details) We have feedback that this antibody does not work in human samples with Western blot.
Purification affinity purified
Immunogen A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. Immunogen has 100% homology to bovine and 88% homology with sheep.Marker Microglia Marker, Myeloid Marke

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN409931) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background Gene Symbol: ITGAM
Gene ID 3684, 16409, 25021
UniProt P05555
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN409931) Images back to top
Application Notes This CD11b antibody is useful for Western blot analysis, Immunocytochemistry, Flow Cytometry (PMID 21422470) and IHC-paraffin embedded sections. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human with Western blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916)
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry 1:400, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1:400, Western Blot 2 µg/mL
Protocol Western Blot protocol specific for CD11b Antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN409931) Images back to top
Format Liquid
Concentration 1.00 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.

References for anti-CD11b Antibody (ABIN409931)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Sakaguchi, Shono, Suzuki, Sawano, Anderson, Do, Ohtsubo, Mizunoya, Sato, Nakamura, Furuse, Yamada, Ikeuchi, Tatsumi: "Implication of anti-inflammatory macrophages in regenerative moto-neuritogenesis: promotion of myoblast migration and neural chemorepellent semaphorin 3A expression in injured muscle." in: The international journal of biochemistry & cell biology, Vol. 54, pp. 272-85, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Sawano, Suzuki, Do, Ohtsubo, Mizunoya, Ikeuchi, Tatsumi: "Supplementary immunocytochemistry of hepatocyte growth factor production in activated macrophages early in muscle regeneration." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 85, Issue 12, pp. 994-1000, 2014

Shono, Sakaguchi, Suzuki, Do, Mizunoya, Nakamura, Sato, Furuse, Yamada, Ikeuchi, Tatsumi: "Preliminary time-course study of antiinflammatory macrophage infiltration in crush-injured skeletal muscle." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 84, Issue 11, pp. 744-50, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Wong, Zhang, Gilkes, Chen, Wei, Chaturvedi, Hubbi, Semenza: "Inhibitors of hypoxia-inducible factor 1 block breast cancer metastatic niche formation and lung metastasis." in: Journal of molecular medicine (Berlin, Germany), Vol. 90, Issue 7, pp. 803-15, 2012 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Jin, Li, Xu, Guo, Chen, Bo: "Lipoxin A4 analog attenuates morphine antinociceptive tolerance, withdrawal-induced hyperalgesia, and glial reaction and cytokine expression in the spinal cord of rat." in: Neuroscience, Vol. 208, pp. 1-10, 2012 (Sample species: Rat (Rattus)). Further details: Western Blotting

Zhang, Kim: "Effect of molecular size and modification pattern on the internalization of water soluble β-(1 → 3)-(1 → 4)-glucan by primary murine macrophages." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 6, pp. 914-27, 2012 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Nelson, Hao, Manos, Ransohoff, Gruol: "Altered hippocampal synaptic transmission in transgenic mice with astrocyte-targeted enhanced CCL2 expression." in: Brain, behavior, and immunity, Vol. 25 Suppl 1, pp. S106-19, 2011 (Sample species: Mouse (Murine)). Further details: Western Blotting

Yin, Wu, Avigan, Rosenblatt, Stone, Kharbanda, Kufe: "MUC1-C oncoprotein suppresses reactive oxygen species-induced terminal differentiation of acute myelogenous leukemia cells." in: Blood, Vol. 117, Issue 18, pp. 4863-70, 2011

Gruol, Puro, Hao, Blakely, Janneke, Vo: "Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6." in: Journal of neuroimmunology, Vol. 239, Issue 1-2, pp. 28-36, 2011 (Sample species: Rat (Rattus)). Further details: Western Blotting

Yin, Ahmad, Kosugi, Kawano, Avigan, Stone, Kharbanda, Kufe: "Terminal differentiation of chronic myelogenous leukemia cells is induced by targeting of the MUC1-C oncoprotein." in: Cancer biology & therapy, Vol. 10, Issue 5, pp. 483-91, 2010 (Sample species: Human). Further details: Immunohistochemistry

Sheldon, Roberts: "Toll-like receptor 4 mediates the response of epithelial and stromal cells to lipopolysaccharide in the endometrium." in: PLoS ONE, Vol. 5, Issue 9, pp. e12906, 2010 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN409931) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD11b antibody (Integrin alpha M) (Internal Region) (ABIN409931) anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody