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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Blocking Reagent (BR)||2 Antibodies||8 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||87 Antibodies|
|Functional Studies (Func)||4 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies|
|Immunofluorescence (IF)||11 Antibodies|
|Immunohistochemistry (IHC)||34 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||7 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||33 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||20 Antibodies|
|Immunoprecipitation (IP)||28 Antibodies|
|Western Blotting (WB)||36 Antibodies|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||3 Antibodies|
|Antigen||Integrin alpha M (ITGAM) Antibodies|
|Epitope||Internal Region, AA 250-350 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CD11b antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
|11 references available|
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Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN409931) Images
|Cross-Reactivity (Details)||We have feedback that this antibody does not work in human samples with Western blot.|
|Immunogen||A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. Immunogen has 100% homology to bovine and 88% homology with sheep.Marker Microglia Marker, Myeloid Marke|
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN409931) Images back to top
|Alternative Name||CD11b (ITGAM Antibody Abstract)|
|Background||Gene Symbol: ITGAM|
|Gene ID||3684, 16409, 25021|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN409931) Images back to top
This CD11b antibody is useful for Western blot analysis, Immunocytochemistry, Flow Cytometry (PMID 21422470) and IHC-paraffin embedded sections. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human with Western blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916)
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry 1:400, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1:400, Western Blot 2 µg/mL
Western Blot protocol specific for CD11b Antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN409931) Images back to top
|Buffer||PBS, 30 % glycerol, Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||Aliquot and store at -20 °C or -80 °C.|
References for anti-CD11b Antibody (ABIN409931)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
|Product cited in:||
Sakaguchi, Shono, Suzuki, Sawano, Anderson, Do, Ohtsubo, Mizunoya, Sato, Nakamura, Furuse, Yamada, Ikeuchi, Tatsumi: "Implication of anti-inflammatory macrophages in regenerative moto-neuritogenesis: promotion of myoblast migration and neural chemorepellent semaphorin 3A expression in injured muscle." in: The international journal of biochemistry & cell biology, Vol. 54, pp. 272-85, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)
Sawano, Suzuki, Do, Ohtsubo, Mizunoya, Ikeuchi, Tatsumi: "Supplementary immunocytochemistry of hepatocyte growth factor production in activated macrophages early in muscle regeneration." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 85, Issue 12, pp. 994-1000, 2014
Shono, Sakaguchi, Suzuki, Do, Mizunoya, Nakamura, Sato, Furuse, Yamada, Ikeuchi, Tatsumi: "Preliminary time-course study of antiinflammatory macrophage infiltration in crush-injured skeletal muscle." in: Animal science journal = Nihon chikusan Gakkaihō, Vol. 84, Issue 11, pp. 744-50, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)
Wong, Zhang, Gilkes, Chen, Wei, Chaturvedi, Hubbi, Semenza: "Inhibitors of hypoxia-inducible factor 1 block breast cancer metastatic niche formation and lung metastasis." in: Journal of molecular medicine (Berlin, Germany), Vol. 90, Issue 7, pp. 803-15, 2012 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry
Jin, Li, Xu, Guo, Chen, Bo: "Lipoxin A4 analog attenuates morphine antinociceptive tolerance, withdrawal-induced hyperalgesia, and glial reaction and cytokine expression in the spinal cord of rat." in: Neuroscience, Vol. 208, pp. 1-10, 2012 (Sample species: Rat (Rattus)). Further details: Western Blotting
Zhang, Kim: "Effect of molecular size and modification pattern on the internalization of water soluble β-(1 → 3)-(1 → 4)-glucan by primary murine macrophages." in: The international journal of biochemistry & cell biology, Vol. 44, Issue 6, pp. 914-27, 2012 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence
Nelson, Hao, Manos, Ransohoff, Gruol: "Altered hippocampal synaptic transmission in transgenic mice with astrocyte-targeted enhanced CCL2 expression." in: Brain, behavior, and immunity, Vol. 25 Suppl 1, pp. S106-19, 2011 (Sample species: Mouse (Murine)). Further details: Western Blotting
Yin, Wu, Avigan, Rosenblatt, Stone, Kharbanda, Kufe: "MUC1-C oncoprotein suppresses reactive oxygen species-induced terminal differentiation of acute myelogenous leukemia cells." in: Blood, Vol. 117, Issue 18, pp. 4863-70, 2011
Gruol, Puro, Hao, Blakely, Janneke, Vo: "Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6." in: Journal of neuroimmunology, Vol. 239, Issue 1-2, pp. 28-36, 2011 (Sample species: Rat (Rattus)). Further details: Western Blotting
Yin, Ahmad, Kosugi, Kawano, Avigan, Stone, Kharbanda, Kufe: "Terminal differentiation of chronic myelogenous leukemia cells is induced by targeting of the MUC1-C oncoprotein." in: Cancer biology & therapy, Vol. 10, Issue 5, pp. 483-91, 2010 (Sample species: Human). Further details: Immunohistochemistry
Sheldon, Roberts: "Toll-like receptor 4 mediates the response of epithelial and stromal cells to lipopolysaccharide in the endometrium." in: PLoS ONE, Vol. 5, Issue 9, pp. e12906, 2010 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry
ImagesProduct Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN409931) back to top