anti-Mouse (Murine) CD11b antibody for Immunohistochemistry (Frozen Sections)

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Reactivity
Human, Mouse (Murine)
704
491
192
78
31
19
17
15
13
11
9
9
7
7
5
4
4
4
2
2
Host
Rat
508
387
166
16
6
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
152
124
92
52
20
19
15
15
14
14
14
13
11
10
9
9
9
8
7
7
7
6
6
6
6
6
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP)
878
233
229
172
170
137
98
97
95
67
25
21
21
13
12
8
5
5
4
3
3
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN120130
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN409930 FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit Internal Region, AA 250-350 Log in to see Polyclonal 24
1 ABIN118146 FACS IHC (fro) IF IHC (p) IP WB Rat IgG2b Log in to see M1-70-15 18
1 ABIN320196 FACS IHC (fro) IP Rat IgG2b Log in to see 5C6 13
1 ABIN370850 FACS IHC (fro) IP Rat IgG2a Log in to see 3A33 11
1 ABIN573960 IHC (fro) FACS IHC (p) IP Rat IgG2b Log in to see M1-70-15
1 ABIN118145 FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70 14
1 ABIN1450195 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see 5C6 12
1 ABIN2478219 IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
1 ABIN2478234 Func IHC (fro) FACS IF IHC (p) IP Rat IgG2b Log in to see M1-70-15 4
1 ABIN4289459 FACS ICC IF IHC (fro) IHC (p) Alexa Fluor 488 Rat IgG2b Log in to see M1-70-15
1 ABIN319553 Func FACS IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15 6
1 ABIN4289448 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit AA 250-350, Internal Region Log in to see Polyclonal 1
1 ABIN370845 FACS IHC (fro) IP Biotin Rat IgG2a Log in to see 3A33 11
1 ABIN1724066 IHC (fro) FACS IHC (p) IP Rat IgG2b Log in to see M1-70-15
1 ABIN1177001 BR IHC (fro) FACS IHC (p) IP WB Rat IgG2b, kappa Log in to see M1-70 8
1 ABIN370849 FACS IHC (fro) IP Rat IgG2a Log in to see 3A33 11
1 ABIN2688908 IHC (f) IHC (fro) FACS Rat IgG2b, kappa Log in to see M1-70 4
1 ABIN439874 FACS ICC IHC IHC (fro) IHC (p) IP in vitro FITC Rat IgG2b kappa Log in to see M1-70
1 ABIN4289434 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15
1 ABIN4289435 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) IP Rat IgG2b Log in to see M1-70-15

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Mouse (Murine)
(704), (491), (192), (78), (31), (19), (17), (15), (13), (11), (9), (9), (7), (7), (5), (4), (4), (4), (2), (2)
Host Rat
(508), (387), (166), (16), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(152), (124), (92), (52), (20), (19), (15), (15), (14), (14), (14), (13), (11), (10), (9), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (6), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP)
(878), (233), (229), (172), (170), (137), (98), (97), (95), (67), (25), (21), (21), (13), (12), (8), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling ProductDetails: References for anti-CD11b antibody (ABIN120130) Images
Purification Ion Exchange Chromatograohy
Immunogen Thioglycollate-elicted Peritoneal Macrophages (TPM). Spleen cells from AO rats were fused with cells of the Y3 rat myeloma cell line.
Clone 5C6
Isotype IgG2b

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling ProductDetails: References for anti-CD11b antibody (ABIN120130) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8 % spleen cells, 44 % bone marrow cells and less than 1 % of thymocytes and is commonly used as a microglial marker in nervous tissue.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 10090
UniProt P11215
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling ProductDetails: References for anti-CD11b antibody (ABIN120130) Images back to top
Application Notes Flow Ccytometry: Use 10 μL of 1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details ProductDetails: References for anti-CD11b antibody (ABIN120130) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD11b antibody (ABIN120130)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Heydenreich, Nolte, Göb, Langhauser, Hofmeister, Kraft, Albert-Weissenberger, Brede, Varallyay, Göbel, Meuth, Nieswandt, Dickneite, Stoll, Kleinschnitz: "C1-inhibitor protects from brain ischemia-reperfusion injury by combined antiinflammatory and antithrombotic mechanisms." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2457-67, 2012

Tysseling, Mithal, Sahni, Birch, Jung, Belmadani, Miller, Kessler: "SDF1 in the dorsal corticospinal tract promotes CXCR4+ cell migration after spinal cord injury." in: Journal of neuroinflammation, Vol. 8, pp. 16, 2011

Wu, Wu, Wang, Wang: "Expression and cellular localization of cyclooxygenases and prostaglandin E synthases in the hemorrhagic brain." in: Journal of neuroinflammation, Vol. 8, pp. 22, 2011

Terwel, Steffensen, Verghese, Kummer, Gustafsson, Holtzman, Heneka: "Critical role of astroglial apolipoprotein E and liver X receptor-α expression for microglial Aβ phagocytosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 19, pp. 7049-59, 2011

McDonald, Cortini, Rosas, Fossati-Jimack, Ling, Lewis, Dewitt, Liddiard, Brown, Jones, Hallett, Botto, Taylor: "In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 6, pp. 1972-82, 2011

Devey, Ferenbach, Mohr, Sangster, Bellamy, Hughes, Wigmore: "Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 17, Issue 1, pp. 65-72, 2008

Basso, Frenkel, Quintana, Costa-Pinto, Petrovic-Stojkovic, Puckett, Monsonego, Bar-Shir, Engel, Gozin, Weiner: "Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis." in: The Journal of clinical investigation, Vol. 118, Issue 4, pp. 1532-43, 2008

Khorooshi, Babcock, Owens: "NF-kappaB-driven STAT2 and CCL2 expression in astrocytes in response to brain injury." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 10, pp. 7284-91, 2008

Hickman, Allison, El Khoury: "Microglial dysfunction and defective beta-amyloid clearance pathways in aging Alzheimer's disease mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 33, pp. 8354-60, 2008

Clausen, Lambertsen, Babcock, Holm, Dagnaes-Hansen, Finsen: "Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice." in: Journal of neuroinflammation, Vol. 5, pp. 46, 2008

Manno: "Measurement of the digit lengths and the anogenital distance in mice." in: Physiology & behavior, Vol. 93, Issue 1-2, pp. 364-8, 2008

Rosen, Milon, Gordon: "Antibody to the murine type 3 complement receptor inhibits T lymphocyte-dependent recruitment of myelomonocytic cells in vivo." in: The Journal of experimental medicine, Vol. 169, Issue 2, pp. 535-48, 1989

Rosen, Gordon: "Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo." in: The Journal of experimental medicine, Vol. 166, Issue 6, pp. 1685-701, 1987

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling ProductDetails: References for anti-CD11b antibody (ABIN120130) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin alpha M (ITGAM) antibody (ABIN120130) Staining of mouse bone marrow with Rat anti Mouse CD11b (ABIN120130)