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anti-Non-Human Primate CD11b antibody for Flow Cytometry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Reactivity
Human, Non-Human Primate, Mouse (Murine), Rabbit
671
498
208
79
31
22
17
15
13
11
9
9
8
8
6
4
4
4
2
Host
Rat
492
396
161
17
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
167
128
93
51
16
15
15
15
14
13
11
11
9
9
9
6
6
6
6
5
5
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
857
246
222
172
163
139
97
95
89
66
26
21
21
19
12
8
6
5
5
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN457402
$ 173.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1981858 Func IHC (f) ICC FACS IP Biotin Mouse IgG1 Log in to see ICRF44 4
1 ABIN1027692 FACS Func ICC IHC (f) IP Mouse IgG1 Log in to see ICRF44 3
1 ABIN2749012 Func IHC (f) ICC FACS IP Mouse IgG1 Log in to see ICRF44 3
1 ABIN457369 FACS Func IHC (f) IP PE Rat IgG2b Log in to see M1-70 9
1 ABIN457346 FACS Func IHC (f) IP FITC Rat IgG2b Log in to see M1-70 8
1 ABIN1690733 FACS Func IHC (f) IP APC Rat IgG2b Log in to see M1-70 10
1 ABIN2749103 Func IHC (f) ICC FACS IP PE Mouse IgG1 Log in to see ICRF44 4
1 ABIN2749102 Func IHC (f) ICC FACS IP FITC Mouse IgG1 Log in to see ICRF44 4

Top referenced anti-CD11b antibody for Flow Cytometry

Similar anti-CD11b Antibodies

Application / Reactivity Rabbit Non-Human Primate
Blocking Reagent (BR) 4 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Flow Cytometry (FACS) 77 Antibodies 9 Antibodies
Functional Studies (Func) 18 Antibodies 9 Antibodies
Immunocytochemistry (ICC) 15 Antibodies 5 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 4 Antibodies 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 33 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 31 Antibodies 9 Antibodies
Western Blotting (WB) 2 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Human, Non-Human Primate, Mouse (Murine), Rabbit
(671), (498), (208), (79), (31), (22), (17), (15), (13), (11), (9), (9), (8), (8), (6), (4), (4), (4), (2)
Host Rat
(492), (396), (161), (17), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(167), (128), (93), (51), (16), (15), (15), (15), (14), (13), (11), (11), (9), (9), (9), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
(857), (246), (222), (172), (163), (139), (97), (95), (89), (66), (26), (21), (21), (19), (12), (8), (6), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN457402) Images
Specificity The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Purification Purified from cell culture supernatant by protein-G affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen B10 mouse spleen cells enriched for T cells
Clone M1-70
Isotype IgG2b

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN457402) Images back to top
Antigen
Alternative Name ITGAM (ITGAM Antibody Abstract)
Background CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, Activation of Innate immune Response

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN457402) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN457402) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD11b Antibody (ABIN457402)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Brickson, Ji, Schell, Olabisi, St Pierre Schneider, Best: "M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 95, Issue 3, pp. 969-76, 2003

Dembic, Schenck, Bogen: "Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 6, pp. 2697-702, 2000

Welt, Edelman, Simon, Rogers: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, 1981