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|Application / Reactivity||Rabbit||Non-Human Primate||Mouse (Murine)|
|Blocking Reagent (BR)||2 Antibodies|
|Flow Cytometry (FACS)||47 Antibodies||9 Antibodies|
|Functional Studies (Func)||12 Antibodies||7 Antibodies|
|Immunofluorescence (IF)||7 Antibodies|
|Immunohistochemistry (IHC)||7 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||14 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies|
|Immunohistochemistry (Paraformaldehyde) (IHC (pfa))||1 Antibodies|
|Immunoprecipitation (IP)||22 Antibodies||7 Antibodies|
|Western Blotting (WB)||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||4 Antibodies|
|Biochemical Assay (BCA)||1 Antibodies|
|Antigen||Integrin alpha M (ITGAM) Antibodies|
|Reactivity||Human, Non-Human Primate, Mouse (Murine), Rabbit Alternatives|
|Conjugate||This CD11b antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
|5 references available|
|Supplier||Log in to see|
Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN457402) Images
|Specificity||The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.|
|Purification||Purified from cell culture supernatant by protein-G affinity chromatography.|
|Purity||> 95 % (by SDS-PAGE)|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.|
|Immunogen||B10 mouse spleen cells enriched for T cells|
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN457402) Images back to top
|Alternative Name||ITGAM (ITGAM Antibody Abstract)|
|Background||CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.|
|Research Area||Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN457402) Images back to top
Flow Cytometry: Recommended dilution: 1 µg/mL
Functional Application: In vitro blocking of CD11b
Working concentrations should be determined by the investigator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN457402) Images back to top
|Buffer||Azide free phosphate buffered saline (PBS), approx. pH 7.4.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-CD11b Antibody (ABIN457402)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
|Product cited in:||
Brickson, Ji, Schell et al.: "M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 95, Issue 3, pp. 969-76, 2003 (PubMed).
Welt, Edelman, Simon et al.: "Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 20, Issue 12, pp. 2553-8, 2000 (PubMed).
Dembic, Schenck, Bogen: "Dendritic cells purified from myeloma are primed with tumor-specific antigen (idiotype) and activate CD4+ T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 6, pp. 2697-702, 2000 (PubMed).
Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).
Ault, Springer: "Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 359-64, 1981 (PubMed).