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|Application / Reactivity||Rat (Rattus)|
|Blocking Reagent (BR)||2 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||87 Antibodies|
|Functional Studies (Func)||4 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies|
|Immunofluorescence (IF)||11 Antibodies|
|Immunohistochemistry (IHC)||34 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||7 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||33 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||20 Antibodies|
|Immunoprecipitation (IP)||28 Antibodies|
|Western Blotting (WB)||39 Antibodies|
|Antigen||Integrin alpha M (ITGAM) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD11b antibody is un-conjugated Alternatives|
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN967477) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN967477) Images back to top
|Alternative Name||CD11b (ITGAM Antibody Abstract)|
The WT.5 antibody reacts with the alpha subunit of Mac-1 (alphaMbeta2, CD11b/CD18) found on neutrophils and myeloid cells, but not on lymphocytes. Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. Mac-1 mediates adhesion to ICAM-1 (CD54) and C3bi. Neutrophil binding to ICAM-1 is blocked by mAb WT.5. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Integrin alphaM chain, Mac-1 alpha chain
|Research Area||Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN967477) Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN967477) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4° C.|
References for anti-CD11b Antibody (ABIN967477)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
|Product cited in:||
Watanabe, Arakawa, Fukuda, Higuchi, Kobayashi: "Role of neutrophils in a rat model of gastric ulcer recurrence caused by interleukin-1 beta." in: The American journal of pathology, Vol. 150, Issue 3, pp. 971-9, 1997
Tamatani, Kitamura, Kuida, Shirao, Mochizuki, Suematsu, Schmid-Schönbein, Watanabe, Tsurufuji, Miyasaka: "Characterization of rat LECAM-1 (L-selectin) by the use of monoclonal antibodies and evidence for the presence of soluble LECAM-1 in rat sera." in: European journal of immunology, Vol. 23, Issue 9, pp. 2181-8, 1993