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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)|
|Cytometry by Time of Flight (CyTOF)||6 Antibodies||2 Antibodies|
|Simple Western (SimWes)||1 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||123 Antibodies||73 Antibodies||52 Antibodies||4 Antibodies|
|Staining Methods (StM)||2 Antibodies||1 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Multiplex Assay (MA)||1 Antibodies|
|Mass Cytometry (CyTOF)||1 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||6 Antibodies||4 Antibodies||2 Antibodies|
|In vitro Assay (in vitro)||1 Antibodies|
|Antigen||Integrin alpha M (ITGAM) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CD11b antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-CD11b AntibodyTarget Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN374760) Images
|Specificity||This antibody detects ITGAM / CD11b.|
Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide with sequence from the internal region of the protein sequence according to NP_000623.2. Genename: ITGAM|
Target Details CD11bProduct Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN374760) Images back to top
|Alternative Name||CD11b (ITGAM Antibody Abstract)|
|Background||CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8 % spleen cells, 44 % bone marrow cells and less than 1 % of thymocytes and is commonly used as a microglial marker in nervous tissue.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor|
|Research Area||Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN374760) Images back to top
Peptide ELISA: 1/32000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN374760) Images back to top
|Buffer||Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD11b Antibody (ABIN374760)Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Zhou, He, Ren, Wang, Zhou, Wan, Ono, Fujiwara, Zuo: "Myeloid suppressor cell-associated immune dysfunction in CSA1M fibrosarcoma tumor-bearing mice." in: Cancer science, Vol. 98, Issue 6, pp. 882-9, 2007
ImagesProduct Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN374760) back to top