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anti-Rat (Rattus) CD11b antibody for Enzyme Immunoassay

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Epitope
Internal Region
32
19
19
14
14
8
6
5
5
5
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
615
466
145
67
26
20
17
17
17
11
9
9
5
4
3
2
2
1
Host
Goat
477
413
117
16
4
2
Clonality
Polyclonal
Conjugate
This CD11b antibody is un-conjugated
164
123
88
51
18
17
12
12
11
11
11
10
10
9
7
6
5
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
800
244
207
162
139
112
78
76
72
69
19
15
11
7
6
5
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN374760
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN782999 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal 1

Top referenced anti-CD11b antibody for Enzyme Immunoassay

Similar anti-CD11b Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 120 Antibodies 49 Antibodies 39 Antibodies 4 Antibodies
Staining Methods (StM) 2 Antibodies 1 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Neutralization (Neut) 1 Antibodies
Multiplex Assay (MA) 1 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 6 Antibodies 4 Antibodies
In vitro Assay (in vitro) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 125 Antibodies 127 Antibodies 28 Antibodies 6 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 57 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Epitope Internal Region
(32), (19), (19), (14), (14), (8), (6), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(615), (466), (145), (67), (26), (20), (17), (17), (17), (11), (9), (9), (5), (4), (3), (2), (2), (1)
Host Goat
(477), (413), (117), (16), (4), (2)
Clonality
Polyclonal
Conjugate This CD11b antibody is un-conjugated
(164), (123), (88), (51), (18), (17), (12), (12), (11), (11), (11), (10), (10), (9), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(800), (244), (207), (162), (139), (112), (78), (76), (72), (69), (19), (15), (11), (7), (6), (5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN374760) Images
Sequence C-RNDGEDSYRT Q
Specificity This antibody detects ITGAM / CD11b.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_000623.2. Genename: ITGAM

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN374760) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8 % spleen cells, 44 % bone marrow cells and less than 1 % of thymocytes and is commonly used as a microglial marker in nervous tissue.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 3684
NCBI Accession NP_000623
UniProt P11215
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN374760) Images back to top
Application Notes Peptide ELISA: 1/32000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN374760) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD11b Antibody (ABIN374760)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Zhou, He, Ren, Wang, Zhou, Wan, Ono, Fujiwara, Zuo: "Myeloid suppressor cell-associated immune dysfunction in CSA1M fibrosarcoma tumor-bearing mice." in: Cancer science, Vol. 98, Issue 6, pp. 882-9, 2007

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN374760) back to top
Supplier Images
 image for anti-CD11b antibody (Integrin alpha M) (Internal Region) (ABIN374760) anti-Integrin alpha M (ITGAM) (Internal Region) antibody