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anti-Rat (Rattus) CD11b antibody for Immunohistochemistry (Frozen Sections)

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Rat (Rattus)
619
467
145
67
26
20
18
17
17
11
10
9
5
4
3
2
2
1
1
Host
Mouse
479
413
117
17
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is un-conjugated
164
124
88
51
18
17
12
12
11
11
11
10
10
9
7
6
5
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
801
243
207
161
141
113
79
76
71
70
19
15
11
7
6
5
4
3
3
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120414
$ 720.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008800383 ABIN409930 FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Rabbit Internal Region, AA 250-350 Log in to see Polyclonal 23
0.0008800383 ABIN120410 FACS IHC (fro) Biotin Mouse IgG2a Log in to see OX-42 3
0.0008800383 ABIN120413 Func FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see OX-42 4
0.0008800383 ABIN120412 Func FACS IHC (fro) IF IHC (p) IP Mouse IgG2a Log in to see OX-42 4
0.0008800383 ABIN182004 FACS IHC (fro) IP Mouse IgG2a Log in to see OX-42 4
0.0008800383 ABIN951178 IHC (fro) Mouse IgG1 Log in to see ED8 1
0.0008800383 ABIN951177 IHC (fro) Mouse IgG1 Log in to see ED8 1
0.0008800383 ABIN119464 FACS IHC (fro) Mouse IgG1 Log in to see ED7 4
0.0008800383 ABIN967477 BR IHC (fro) FACS IP WB Mouse IgA, kappa Log in to see WT-5 2
0.0008800383 ABIN4289448 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 488 Rabbit AA 250-350, Internal Region Log in to see Polyclonal 1
0.0008800383 ABIN1859498 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 598-700 Log in to see Polyclonal
0.0008800383 ABIN1859499 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 870-1004 Log in to see Polyclonal
0.0008800383 ABIN1859494 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 31-160 Log in to see Polyclonal
0.0008800383 ABIN213152 FACS IHC (fro) Mouse IgG1 Log in to see ED7
0.0008800383 ABIN600694 IHC (fro) FACS Mouse IgG1 Log in to see
0.0008800383 ABIN284103 FACS IHC (fro) Mouse IgG2a Log in to see OX-42
0.0008800383 ABIN198550 FACS IHC (fro) Biotin Mouse IgG2a Log in to see
0.0008800383 ABIN206706 FACS IHC (fro) Biotin Mouse IgG2a Log in to see OX-42
0.0008800383 ABIN600700 IHC (fro) FACS IP Mouse IgG2a Log in to see
0.0008800383 ABIN2478198 IHC (fro) FACS IF IP Mouse IgG2a Log in to see OX-42 4

Top referenced anti-CD11b antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CD11b Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 2 Antibodies
ELISA 13 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 87 Antibodies
Functional Studies (Func) 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 33 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 28 Antibodies
Western Blotting (WB) 39 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Rat (Rattus)
(619), (467), (145), (67), (26), (20), (18), (17), (17), (11), (10), (9), (5), (4), (3), (2), (2), (1), (1)
Host Mouse
(479), (413), (117), (17), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is un-conjugated
(164), (124), (88), (51), (18), (17), (12), (12), (11), (11), (11), (10), (10), (9), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(801), (243), (207), (161), (141), (113), (79), (76), (71), (70), (19), (15), (11), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN120414) Images
Purification Affinity chromatography on Protein G
Immunogen Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Clone OX-42
Isotype IgG2a

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN120414) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35 % of alveolar macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 10116
UniProt Q63001
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN120414) Images back to top
Application Notes Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/50 - 1/100. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN120414) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD11b Antibody (ABIN120414)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Background publications

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Milligan, Cunningham, Levitt: "Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain." in: The Journal of comparative neurology, Vol. 314, Issue 1, pp. 125-35, 1992

Robinson, White, Mason: "Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3." in: Immunology, Vol. 57, Issue 2, pp. 239-47, 1986

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN120414) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD11b antibody (Integrin alpha M) (ABIN120414) Staining of rat peritoneal macrophages with MOUSE ANTI RAT CD11b(ABIN120414).