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anti-Rhesus Monkey CD11b antibody for Flow Cytometry

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • C3bi
  • CD11B
  • Cd11b
  • CD11b/CD18
  • CR3
  • CR3A
  • F730045J24Rik
  • Ly-40
  • MAC-1
  • Mac-1
  • Mac-1a
  • MAC1
  • MAC1A
  • MO1A
  • SLEB6
Reactivity
Baboon, Chimpanzee, Cynomolgus, Human, Rhesus Monkey
551
431
133
48
26
20
16
16
16
10
10
9
5
4
3
2
2
1
Host
Mouse
446
390
103
16
4
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to PerCP-eFluor® 710
162
120
85
51
18
17
12
11
11
8
7
7
7
7
6
6
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
757
219
177
132
108
82
75
67
48
38
19
15
11
7
6
5
4
3
3
3
3
3
2
1
1
1
Supplier
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Available images

Catalog No. ABIN2677355
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.26371998 ABIN2677345 FACS PE-Cy5 Mouse IgG1 kappa Log in to see ICRF44 1
0.26371998 ABIN2677339 FACS FITC Mouse IgG1 kappa Log in to see ICRF44
0.26371998 ABIN2677349 FACS APC Mouse IgG1 kappa Log in to see ICRF44
0.26371998 ABIN2677357 FACS APC eFluor 780 Mouse IgG1 kappa Log in to see ICRF44
0.26371998 ABIN2677343 FACS PE Mouse IgG1 kappa Log in to see ICRF44
0.26371998 ABIN2677351 FACS PE-Cy7 Mouse IgG1 kappa Log in to see ICRF44
0.0010444935 ABIN280223 Func FACS IHC (fro) IHC Mouse IgG1 kappa Log in to see ICRF44
0.0010444935 ABIN2478194 FACS PE-Alexa Fluor 647 Mouse IgG1 Log in to see ICRF44 2
0.0010444935 ABIN2478187 FACS Alexa Fluor 700 Mouse IgG1 Log in to see ICRF44 2
0.0010444935 ABIN2478192 FACS PE Mouse IgG1 Log in to see ICRF44 2
0.0010444935 ABIN2478183 Func IHC (fro) FACS IP Mouse IgG1 Log in to see ICRF44 2
0.0010444935 ABIN2478189 FACS FITC Mouse IgG1 Log in to see ICRF44 2
0.0010444935 ABIN2478185 FACS Alexa Fluor 647 Mouse IgG1 Log in to see ICRF44 2
0.0010444935 ABIN279372 FACS Biotin Mouse IgG1 kappa Log in to see ICRF44
0.0010444935 ABIN3024273 BR FACS IF Rat IgG2b, kappa Log in to see M1-70
0.0010444935 ABIN3024274 BR FACS IF Rat IgG2b, kappa Log in to see M1-70

Top referenced anti-CD11b antibody for Flow Cytometry

Similar anti-CD11b Antibodies

Application / Reactivity Human Baboon Cynomolgus Rhesus Monkey Chimpanzee
Western Blotting (WB) 89 Antibodies
Staining Methods (StM) 1 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Multiplex Assay (MA) 1 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 6 Antibodies
Immunoprecipitation (IP) 101 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies
Immunohistochemistry (IHC) 93 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 67 Antibodies 2 Antibodies 2 Antibodies

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Baboon, Chimpanzee, Cynomolgus, Human, Rhesus Monkey
(551), (431), (133), (48), (26), (20), (16), (16), (16), (10), (10), (9), (5), (4), (3), (2), (2), (1)
Host Mouse
(446), (390), (103), (16), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is conjugated to PerCP-eFluor® 710
(162), (120), (85), (51), (18), (17), (12), (11), (11), (8), (7), (7), (7), (7), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(757), (219), (177), (132), (108), (82), (75), (67), (48), (38), (19), (15), (11), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN2677355) Images
Specificity The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin β2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.
Characteristics The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin β2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.
Synonyms: Synonyms: Integrin alpha M, ITGAM, Mac-1 alpha (Mac1A), Complement Receptor 3 alpha (CR3A)
Clone ICRF44
Isotype IgG1 kappa

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b Antibody (ABIN2677355) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b Antibody (ABIN2677355) Images back to top
Application Notes Applications reported: This ICRF44 antibody has been reported for use in flow cytometric analysis.

Applications tested: This ICRF44 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Fixation: Samples can be stored in IC Fixation Buffer (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4 °C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b Antibody (ABIN2677355) Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

References for anti-CD11b Antibody (ABIN2677355)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Moreau, Hill, Thébault, Deschamps, Chiffoleau, Chauveau, Moullier, Anegon, Alliot-Licht, Cuturi: "Tolerogenic dendritic cells actively inhibit T cells through heme oxygenase-1 in rodents and in nonhuman primates." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 9, pp. 3070-7, 2009

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b Antibody (ABIN2677355) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD11b antibody (Integrin alpha M)  (PerCP-eFluor® 710) (ABIN2677355) anti-Integrin alpha M (ITGAM) antibody (PerCP-eFluor® 710)