anti-Human CFLAR antibody for Immunofluorescence

Recommended CFLAR Antibody (supplied by: Log in to see )

Antigen
CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
  • Clarp1
  • cflar
  • CFLAR
  • FLIP
  • CASH
  • CASP8AP1
  • CLARP
  • Casper
  • FLAME
  • FLAME-1
  • FLAME1
  • I-FLICE
  • MRIT
  • c-FLIP
  • c-FLIPL
  • c-FLIPR
  • c-FLIPS
  • 2310024N18Rik
  • A430105C05Rik
  • Cash
  • ENSMUSG00000072980
  • Flip
  • Gm9845
  • c-Flip
  • CASP8 and FADD-like apoptosis regulator a
  • CASP8 and FADD-like apoptosis regulator
  • cellular FLICE-like inhibitory protein
  • cflara
  • CFLAR
  • cflar
  • Cflar
  • C-FLIP
Epitope
C-Term
41
36
24
23
17
16
15
14
10
7
6
6
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
280
135
90
6
2
2
1
1
Host
Rabbit
161
112
23
Clonality
Polyclonal
Conjugate
This CFLAR antibody is un-conjugated
11
8
7
6
6
4
4
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
257
120
102
42
38
29
29
26
21
8
4
2
2
1
1
1
1
Supplier
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Catalog No. ABIN4312113
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.783466 ABIN4312114 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
11.783466 ABIN781988 EIA IF IHC (p) WB Mouse IgG2a Log in to see 2F12
11.783466 ABIN4312110 ELISA ICC IF WB Rabbit C-Term Log in to see Polyclonal
11.783466 ABIN4312111 ELISA ICC IF WB Rabbit C-Term Log in to see Polyclonal
11.783466 ABIN615235 EIA IF IHC (p) WB Rabbit AA 191-209 Log in to see Polyclonal
11.783466 ABIN4312112 ELISA FACS ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
11.783466 ABIN563829 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 178-277, partial Log in to see 2F12
11.783466 ABIN522287 IF IP ELISA WB Mouse IgG2a kappa AA 178-277, partial Log in to see 1G1
1 ABIN222911 IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN318973 IHC (fro) IF WB Rabbit IgG C-Term, AA 191-209 Log in to see Polyclonal 4
1 ABIN449670 ELISA ICC IF SDS WB Mouse IgG3 kappa AA 1-376 Log in to see 5D8
1 ABIN5554801 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5554800 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN5554802 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN395575 IF WB Mouse IgG2a kappa AA 178-277 Log in to see 2F12
1 ABIN768891 IF IHC (p) ELISA WB Mouse IgG2a, kappa AA 178-277 Log in to see 2F12
1 ABIN2471477 IF IHC ELISA WB Mouse IgG2a kappa AA 178-277 Log in to see 2F12
1 ABIN4965081 IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1723783 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 178-277 Log in to see 2F12
1 ABIN121511 IF WB Rabbit C-Term, long form Log in to see Polyclonal

General

Antigen CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
Epitope C-Term
(41), (36), (24), (23), (17), (16), (15), (14), (10), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(280), (135), (90), (6), (2), (2), (1), (1)
Host Rabbit
(161), (112), (23)
Clonality
Polyclonal
Conjugate This CFLAR antibody is un-conjugated
(11), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(257), (120), (102), (42), (38), (29), (29), (26), (21), (8), (4), (2), (2), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CFLAR Antibody

Target Details CFLAR Application Details Handling References for anti-CFLAR antibody (ABIN4312113) Images
Specificity FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
Purification Ion exchange chromatography
Immunogen Antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP. The immunogen is located within the last 50 amino acids of FLIP.

Target Details CFLAR

Product Details anti-CFLAR Antibody Application Details Handling References for anti-CFLAR antibody (ABIN4312113) Images back to top
Antigen
Alternative Name FLIP (CFLAR Antibody Abstract)
Background Gene Symbol: CFLAR
Molecular Weight Theoretical MW: 58 kDa
Gene ID 8837
NCBI Accession NP_997536
Research Area Signaling, Apoptosis/Necrosis, Cancer
Pathways Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development

Application Details

Product Details anti-CFLAR Antibody Target Details CFLAR Handling References for anti-CFLAR antibody (ABIN4312113) Images back to top
Application Notes Western Blot 1-2 μg/mL, ELISA 1:100-1:2000, Immunohistochemistry 5 μg/mL, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5 μg/mLFLIP antibody can be used for the detection of FLIP by Western blot at 1:500 to 1:1000 dilution. 55 kDa band can be detected. FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details References for anti-CFLAR antibody (ABIN4312113) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CFLAR antibody (ABIN4312113)

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling Images back to top
Product cited in:

Kim, Gupta, Park, Yadav, Aggarwal et al.: "Turmeric (Curcuma longa) inhibits inflammatory nuclear factor (NF)-κB and NF-κB-regulated gene products and induces death receptors leading to suppressed proliferation, induced chemosensitization, ..." in: Molecular nutrition & food research, Vol. 56, Issue 3, pp. 454-65, 2012

Phromnoi, Reuter, Sung, Prasad, Kannappan, Yadav, Chanmahasathien, Limtrakul, Aggarwal et al.: "A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IκB kinase activation and sensitizes tumor cells to apoptosis by ..." in: Molecular pharmacology, Vol. 79, Issue 2, pp. 279-89, 2011

Takada, Andreeff, Aggarwal et al.: "Indole-3-carbinol suppresses NF-kappaB and IkappaBalpha kinase activation, causing inhibition of expression of NF-kappaB-regulated antiapoptotic and metastatic gene products and enhancement of ..." in: Blood, Vol. 106, Issue 2, pp. 641-9, 2005

Aggarwal, Ichikawa, Takada, Sandur, Shishodia, Aggarwal: "Curcumin (diferuloylmethane) down-regulates expression of cell proliferation and antiapoptotic and metastatic gene products through suppression of IkappaBalpha kinase and Akt activation." in: Molecular pharmacology, Vol. 69, Issue 1, pp. 195-206, 2005

Ichikawa, Takada, Murakami, Aggarwal: "Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 7383-92, 2005

Takada, Khuri, Aggarwal et al.: "Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression ..." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26287-99, 2004

Takada, Fang, Jamaluddin, Boyd, Aggarwal: "Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 39541-54, 2004

Kishimoto, Sprent: "A defect in central tolerance in NOD mice." in: Nature immunology, Vol. 2, Issue 11, pp. 1025-31, 2001

Kim, Fisher, Xu, el-Deiry: "Molecular determinants of response to TRAIL in killing of normal and cancer cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 6, Issue 2, pp. 335-46, 2000

Images

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling References for anti-CFLAR antibody (ABIN4312113) back to top
Supplier Images
Immunofluorescence (IF) image for anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (C-Term) antibody (ABIN4312113) Immunocytochemistry/Immunofluorescence: FLIP Antibody [NBP1-77020] - 3T3 cells with F...
Western Blotting (WB) image for anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (C-Term) antibody (ABIN4312113) Western Blot: FLIP Antibody [NBP1-77020] - Western blot analysis of FLIP in NIH/3T3 w...
Western Blotting (WB) image for anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (C-Term) antibody (ABIN4312113) Western Blot: FLIP Antibody [NBP1-77020] - Analysis of FLIP in K562 cell lysate with ...