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anti-Human CFLAR antibody for Immunofluorescence

Recommended CFLAR Antibody (supplied by: Log in to see )

Antigen
CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
  • 2310024N18Rik
  • A430105C05Rik
  • c-Flip
  • c-FLIP
  • c-FLIPL
  • c-FLIPR
  • c-FLIPS
  • CASH
  • Cash
  • CASP8AP1
  • Casper
  • cflar
  • CFLAR
  • CLARP
  • Clarp1
  • ENSMUSG00000072980
  • FLAME
  • FLAME-1
  • FLAME1
  • Flip
  • FLIP
  • Gm9845
  • I-FLICE
  • MRIT
Epitope
N-Term
37
21
18
13
13
13
10
8
7
6
6
6
6
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
199
78
56
2
2
2
2
2
1
Host
Rabbit
155
60
4
Clonality
Polyclonal
Conjugate
This CFLAR antibody is un-conjugated
7
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
179
73
48
36
34
22
22
15
13
7
3
3
1
1
1
Supplier
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Catalog No. ABIN499846
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3124413 ABIN499850 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.3124413 ABIN499848 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.3124413 ABIN781988 EIA IF IHC (p) WB Mouse IgG2a Log in to see 2F12
0.3124413 ABIN615235 EIA IF IHC (p) WB Rabbit AA 191-209 Log in to see Polyclonal
0.3124413 ABIN563829 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 178-277, partial Log in to see 2F12
0.3124413 ABIN522287 IF IP ELISA WB Mouse IgG2a kappa AA 178-277, partial Log in to see 1G1
0.00109224 ABIN318973 IHC (fro) IF WB Rabbit IgG C-Term, AA 191-209 Log in to see Polyclonal 4
0.00109224 ABIN222911 IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.00109224 ABIN395575 IF WB Mouse IgG2a kappa AA 178-277 Log in to see 2F12
0.00109224 ABIN2471477 IF IHC ELISA WB Mouse IgG2a kappa AA 178-277 Log in to see 2F12
0.00109224 ABIN768891 IF IHC (p) ELISA WB Mouse IgG2a, kappa Log in to see 2F12
0.00109224 ABIN241636 IF WB Rabbit C-Term Log in to see Polyclonal
0.00109224 ABIN1723783 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 178-277 Log in to see 2F12
0.00109224 ABIN121511 IF WB Rabbit C-Term, long form Log in to see Polyclonal
0.00109224 ABIN1031381 FACS IF IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.00109224 ABIN1888196 FACS IF IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.00109224 ABIN2232347 IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.00109224 ABIN320424 IF WB Rabbit IgG C-Term, long form Log in to see Polyclonal
0.00109224 ABIN2140243 IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.00109224 ABIN2232334 IF IHC ELISA WB Mouse IgG2a kappa AA 178-277 Log in to see 2F12

Similar anti-CFLAR Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 17 Antibodies 26 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 4 Antibodies
Flow Cytometry (FACS) 2 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 13 Antibodies
Immunofluorescence (IF) 5 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 10 Antibodies 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 10 Antibodies
SDS-PAGE (SDS) 1 Antibodies
Western Blotting (WB) 36 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies

General

Antigen CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
Epitope N-Term
(37), (21), (18), (13), (13), (13), (10), (8), (7), (6), (6), (6), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(199), (78), (56), (2), (2), (2), (2), (2), (1)
Host Rabbit
(155), (60), (4)
Clonality
Polyclonal
Conjugate This CFLAR antibody is un-conjugated
(7), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(179), (73), (48), (36), (34), (22), (22), (15), (13), (7), (3), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CFLAR Antibody

Target Details CFLAR Application Details Handling References for anti-CFLAR Antibody (ABIN499846) Images
Specificity FLIP antibody was raised against a peptide corresponding to amino acids near the amino terminus of human FLIP The sequence is identical in all FLIP splice variants. FLIP recognizes all FLIP splice variants including FLIPa, b, and y.
Purification Affinity chromatography purified via peptide column
Immunogen Bacteria FLIP (N-Terminus) Peptide
Isotype IgG

Target Details CFLAR

Product Details anti-CFLAR Antibody Application Details Handling References for anti-CFLAR Antibody (ABIN499846) Images back to top
Antigen
Alternative Name CFLAR / Casper / I-FLICE (CFLAR Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP
Gene ID 8837
UniProt O15519
Pathways Apoptosis

Application Details

Product Details anti-CFLAR Antibody Target Details CFLAR Handling References for anti-CFLAR Antibody (ABIN499846) Images back to top
Application Notes ELISA. Western Blot: 1/500 to 1/1000. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details References for anti-CFLAR Antibody (ABIN499846) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-CFLAR Antibody (ABIN499846)

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling Images back to top
Background publications

Thome, Schneider, Hofmann, Fickenscher, Meinl, Neipel, Mattmann, Burns, Bodmer, Schröter, Scaffidi, Krammer, Peter, Tschopp: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997

Irmler, Thome, Hahne, Schneider, Hofmann, Steiner, Bodmer, Schröter, Burns, Mattmann, Rimoldi, French, Tschopp: "Inhibition of death receptor signals by cellular FLIP." in: Nature, Vol. 388, Issue 6638, pp. 190-5, 1997

Shu, Halpin, Goeddel: "Casper is a FADD- and caspase-related inducer of apoptosis." in: Immunity, Vol. 6, Issue 6, pp. 751-63, 1997

Hu, Vincenz, Ni, Gentz, Dixit: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997

Images

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling References for anti-CFLAR Antibody (ABIN499846) back to top
Supplier Images
Immunofluorescence (IF) image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (N-Term) (ABIN499846) Immunocytochemistry of FLIP in HeLa cells with AP30341PU-N FLIP antibody at 5 μg/ml.
Western Blotting (WB) image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (N-Term) (ABIN499846) Western blot analysis of FLIP in K562 cell lysate with AP30341PU-N FLIP antibody at (...