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anti-Mouse (Murine) CFLAR antibody for Immunofluorescence

Recommended CFLAR Antibody (supplied by: Log in to see )

Antigen
CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
  • 2310024N18Rik
  • A430105C05Rik
  • c-Flip
  • c-FLIP
  • c-FLIPL
  • c-FLIPR
  • c-FLIPS
  • CASH
  • Cash
  • CASP8AP1
  • Casper
  • cflar
  • CFLAR
  • CLARP
  • Clarp1
  • ENSMUSG00000072980
  • FLAME
  • FLAME-1
  • FLAME1
  • Flip
  • FLIP
  • Gm9845
  • I-FLICE
  • MRIT
Epitope
C-Term
36
21
19
13
13
13
10
8
7
6
6
6
6
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
200
78
57
2
2
2
2
2
1
Host
Rabbit
155
60
4
Clonality
Polyclonal
Conjugate
This CFLAR antibody is un-conjugated
7
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
179
73
48
36
34
22
22
15
13
7
3
3
1
1
1
Supplier
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Catalog No. ABIN499844
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.28855592 ABIN499846 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 4
0.28855592 ABIN499848 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.0010854929 ABIN318973 IHC (fro) IF WB Rabbit IgG C-Term, AA 191-209 Log in to see Polyclonal 4
0.0010854929 ABIN222911 IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010854929 ABIN1031381 FACS IF IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0010854929 ABIN1888196 FACS IF IHC ELISA WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-CFLAR antibody for Immunofluorescence

Similar anti-CFLAR Antibodies

Application / Reactivity Mouse (Murine)
ELISA 26 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 2 Antibodies
SDS-PAGE (SDS) 1 Antibodies
Western Blotting (WB) 57 Antibodies

General

Antigen CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
Epitope C-Term
(36), (21), (19), (13), (13), (13), (10), (8), (7), (6), (6), (6), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(200), (78), (57), (2), (2), (2), (2), (2), (1)
Host Rabbit
(155), (60), (4)
Clonality
Polyclonal
Conjugate This CFLAR antibody is un-conjugated
(7), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(179), (73), (48), (36), (34), (22), (22), (15), (13), (7), (3), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CFLAR Antibody

Target Details CFLAR Application Details Handling References for anti-CFLAR Antibody (ABIN499844) Images
Specificity FLIP antibody was raised against a peptide corresponding to amino acids 449 to 465 of mouse FLIPL/CASHa. FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
Purification DEAE
Immunogen Bacteria FLIP (C-Terminus) Peptide
Isotype IgG

Target Details CFLAR

Product Details anti-CFLAR Antibody Application Details Handling References for anti-CFLAR Antibody (ABIN499844) Images back to top
Antigen
Alternative Name CFLAR / Casper / I-FLICE (CFLAR Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP
Gene ID 12633
NCBI Accession NP_997536
UniProt O35732
Pathways Apoptosis

Application Details

Product Details anti-CFLAR Antibody Target Details CFLAR Handling References for anti-CFLAR Antibody (ABIN499844) Images back to top
Application Notes ELISA. Western Blot: 1/500 to 1/1000.55 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details References for anti-CFLAR Antibody (ABIN499844) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-CFLAR Antibody (ABIN499844)

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling Images back to top
Background publications

Thome, Schneider, Hofmann, Fickenscher, Meinl, Neipel, Mattmann, Burns, Bodmer, Schröter, Scaffidi, Krammer, Peter, Tschopp: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997

Irmler, Thome, Hahne, Schneider, Hofmann, Steiner, Bodmer, Schröter, Burns, Mattmann, Rimoldi, French, Tschopp: "Inhibition of death receptor signals by cellular FLIP." in: Nature, Vol. 388, Issue 6638, pp. 190-5, 1997

Shu, Halpin, Goeddel: "Casper is a FADD- and caspase-related inducer of apoptosis." in: Immunity, Vol. 6, Issue 6, pp. 751-63, 1997

Hu, Vincenz, Ni, Gentz, Dixit: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997

Images

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling References for anti-CFLAR Antibody (ABIN499844) back to top
Supplier Images
Immunofluorescence (IF) image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (C-Term) (ABIN499844) Immunocytochemistry of FLIP in 3T3 cells with AP30340PU-N FLIP antibody at 5 μg/ml.
Western Blotting (WB) image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (C-Term) (ABIN499844) Western blot analysis of FLIP in NIH/3T3 whole cell lysate with AP30340PU-N FLIP anti...