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Human Clusterin ELISA Kit

Recommended Clusterin Product (supplied by: Log in to see )

Antigen
Clusterin (CLU) ELISA Kits
  • 40
  • AI893575
  • APO-J
  • APOJ
  • ApoJ
  • Cli
  • CLI
  • CLU
  • clu
  • CLU1
  • CLU2
  • D14Ucla3
  • DAG
  • GP80
  • gpIII
  • KUB1
  • LOC100136120
  • MGC53055
  • MGC89163
  • NA1/NA2
  • RATTRPM2B
  • SGP-2
  • Sgp-2
  • SGP2
  • Sgp2
  • SP-40
  • SP40
  • Sugp-2
  • TRPM-2
  • Trpm2
  • TRPM2
  • TRPM2B
  • Trpmb
  • xclu
  • zgc:73352
Epitope
AA 23-227, AA 228-449
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
20
20
15
7
6
5
5
5
4
3
3
3
2
1
1
Methode Type
Sandwich ELISA
Detection Range
0.78-50 ng/mL
Minimum Detection Limit
0.78 ng/mL
Application
ELISA
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Catalog No. ABIN1672832
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN414571 Sandwich ELISA Serum, Plasma, Urine, Saliva, Tissue Homogenate, Cerebrospinal Fluid, Cell Culture Supernatant, Biological Fluids 12.5-800 ng/mL 12.5 ng/mL Log in to see 2
0.32693866 ABIN1114201 Sandwich ELISA Serum, Plasma, Biological Fluids 3.13-200 ng/mL 3.13 ng/mL Log in to see
0.32693866 ABIN1380020 Sandwich ELISA Cell Lysate, Serum, Plasma 63-4000 pg/mL 63 pg/mL Log in to see
0.32693866 ABIN2708172 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Urine 780-50000 pg/mL 780 pg/mL Log in to see
0.0012117105 ABIN366947 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Saliva, Urine 3.12-200 ng/mL 3.12 ng/mL Log in to see 1
0.0012117105 ABIN3032905 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.9-20000 pg/mL 4.9 pg/mL Log in to see
0.0012117105 ABIN509890 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 25-500 ng/mL 25 ng/mL Log in to see
0.0012117105 ABIN3198500 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.25-2000 ng/mL 31.25 ng/mL Log in to see
0.0012117105 ABIN454746 Plasma, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Log in to see
0.0012117105 ABIN1028385 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.781-50 ng/mL 0.781 ng/mL Log in to see
0.0012117105 ABIN1979436 Serum, Plasma, Cell Culture Supernatant, Urine Log in to see
0.0012117105 ABIN2042407 Sandwich ELISA 0.8-50 ng/mL 0.8 ng/mL Log in to see
0.0012117105 ABIN2065131 Cell Lysate, Serum, Plasma 63-4000 pg/mL 63 pg/mL Log in to see
0.0012117105 ABIN2683832 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Saliva, Serum, Urine 0.78-50 ng/mL 0.78 ng/mL Log in to see
0.0012117105 ABIN2683833 Sandwich ELISA Plasma (EDTA), Media, Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN2543055 Serum, Plasma, Urine, Saliva, Tissue Homogenate, Cerebrospinal Fluid, Cell Culture Supernatant, Biological Fluids 18-800 ng/mL 18 ng/mL Log in to see
0.0012117105 ABIN2543058 Sandwich ELISA Serum, Plasma, Urine, Saliva, Tissue Homogenate, Cerebrospinal Fluid, Cell Culture Supernatant, Biological Fluids 62.5-4000 ng/mL 62.5 ng/mL Log in to see
0.0012117105 ABIN2946416 3.125-200 ng/mL 3.125 ng/mL Log in to see
0.0012117105 ABIN2074788 Sandwich ELISA Log in to see
0.0012117105 ABIN2871381 Sandwich ELISA Log in to see

Top referenced Clusterin ELISA Kit for ELISA

  • Human Clusterin ELISA Kit for Sandwich ELISA - ABIN414571 (2 Publications): Yu, Chen, Wang, Zhou, Luo, Xu, Shen, Yin, Tao, Xiao, Xiao, Lü, Wang: Time-dependent decrease of clusterin as a potential cerebrospinal fluid biomarker for drug-resistant epilepsy. in Journal of molecular neuroscience : MN 2014 (PubMed)

  • Human Clusterin ELISA Kit for Sandwich ELISA - ABIN366947 (1 Publications): Tzounakas, Georgatzakou, Kriebardis, Papageorgiou, Stamoulis, Foudoulaki-Paparizos, Antonelou, Papassideri: Uric acid variation among regular blood donors is indicative of red blood cell susceptibility to storage lesion markers: A new hypothesis tested. in Transfusion 2015 (PubMed)

  • Human Clusterin ELISA Kit for Sandwich ELISA - ABIN1672832 (1 Publications): Han, DeMattos, Dugan, Kim-Han, Brendza, Fryer, Kierson, Cirrito, Quick, Harmony, Aronow, Holtzman: Clusterin contributes to caspase-3-independent brain injury following neonatal hypoxia-ischemia. in Nature medicine 2001 (PubMed)

Similar Clusterin ELISA Kits

Application / Reactivity Human
ELISA 21 ELISA Kits

General

Antigen Clusterin (CLU) ELISA Kits
Epitope AA 23-227, AA 228-449
Reactivity Human
Kits with alternative reactivity to:
(20), (20), (15), (7), (6), (5), (5), (5), (4), (3), (3), (3), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 0.78-50 ng/mL
Minimum Detection Limit 0.78 ng/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details Clusterin ELISA Kit

Target details Application Details Handling References for Clusterin Kit (ABIN1672832) Images
Purpose For quantitative detection of human Clusterin in cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine
Detection Method Colorimetric
Specificity Natural and recombinant human Clusterin
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <20pg/mL
Components
  • 96-well plate precoated with anti- human Clusterin antibod - 1
  • Lyophilized recombinant human Clusterin standar - 50ng/tubex2
  • Biotinylated anti- human Clusterin antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: D23-R227(beta)&S228-E449(alpha)

Target details

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Antigen
Alternative Name CLU (CLU ELISA Kit Abstract)
Background Clusterin(apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein(DAG protein), testosterone repressed prostate message-2(TRPM-2), sulfated glycoprotein-2(SGP-2) and complement lysis inhibitor(CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic(H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.
Gene ID 1191
UniProt P10909
Pathways Apoptosis

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human Clusterin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Clusterin has been precoated onto 96-well plates. Standards(NSO, D23-R227(β)&S228-E449(α)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Clusterin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Clusterin amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Clusterin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Clusterin standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 5.62, Standard deviation: 0.259, CV(%): 4.6
  • Sample 2: n=16, Mean(ng/ml): 10.1, Standard deviation: 0.444, CV(%): 4.4
  • Sample 3: n=16, Mean(ng/ml): 25.5, Standard deviation: 1.07, CV(%): 4.2,
  • Sample 1: n=24, Mean(ng/ml): 6.23, Standard deviation: 0.467, CV(%): 7.5
  • Sample 2: n=24, Mean(ng/ml): 12.1, Standard deviation: 0.835, CV(%): 6.9
  • Sample 3: n=24, Mean(ng/ml): 26.4, Standard deviation: 1.95, CV(%): 7.4
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Clusterin Kit (ABIN1672832)

Product Details Clusterin ELISA Kit Target details Application Details Handling Images back to top
Background publications

Han, DeMattos, Dugan, Kim-Han, Brendza, Fryer, Kierson, Cirrito, Quick, Harmony, Aronow, Holtzman: "Clusterin contributes to caspase-3-independent brain injury following neonatal hypoxia-ischemia." in: Nature medicine, Vol. 7, Issue 3, pp. 338-43, 2001

Images

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Supplier Images
ELISA image for Clusterin ELISA Kit (CLU) (ABIN1672832) Human Clusterin PicoKine ELISA Kit standard curve