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anti-Human CRADD antibody for Western Blotting

Recommended CRADD Antibody (supplied by: Log in to see )

Antigen
CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD) Antibodies
  • cradd
  • CRADD
  • DKFZp468A2219
  • LOC100226369
  • MGC78788
  • MGC89035
  • MRT34
  • RAIDD
  • raidd
  • zgc:101717
Epitope
AA 99-117, Middle Region
19
18
11
11
11
8
8
7
3
3
2
1
1
1
1
Reactivity
Human
145
16
15
2
2
1
1
1
1
Host
Rabbit
108
36
2
1
Clonality
Polyclonal
Conjugate
This CRADD antibody is un-conjugated
9
9
7
4
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
132
72
27
16
12
9
9
2
2
1
1
Supplier
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Catalog No. ABIN500561
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12650052 ABIN500563 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 3
0.12650052 ABIN222892 WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN2706923 WB Rabbit Center Log in to see Polyclonal
0.12650052 ABIN1538574 WB Rabbit Ig AA 69-98, Center Log in to see Polyclonal
0.12650052 ABIN393523 IF WB Mouse IgG1 kappa AA 1-200 Log in to see 1F8
0.12650052 ABIN1871993 IHC WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN2160305 WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.12650052 ABIN2476316 WB Rabbit IgG Log in to see Polyclonal 1
0.12650052 ABIN3021483 IHC WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN3201804 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 1-199 Log in to see
0.12650052 ABIN2166892 WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN121568 IF WB Rabbit AA 99-117 Log in to see Polyclonal
0.12650052 ABIN2166893 WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN563759 IF ELISA WB Mouse IgG1 kappa AA 1-199, full length Log in to see 1F8
0.12650052 ABIN1031051 ICC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.12650052 ABIN1030612 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.12650052 ABIN187724 WB Rabbit AA 99-117 Log in to see Polyclonal
0.12650052 ABIN187725 WB Rabbit AA 181-199 Log in to see Polyclonal
0.12650052 ABIN2403922 IHC WB Rabbit IgG Log in to see Polyclonal
0.12650052 ABIN2498252 ICC ELISA WB Rabbit AA 99-117 Log in to see Polyclonal

Top referenced anti-CRADD antibody for Western Blotting

Similar anti-CRADD Antibodies

Application / Reactivity Human
ELISA 72 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 17 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 27 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 132 Antibodies

General

Antigen CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD) Antibodies
Epitope AA 99-117, Middle Region
(19), (18), (11), (11), (11), (8), (8), (7), (3), (3), (2), (1), (1), (1), (1)
Reactivity Human
(145), (16), (15), (2), (2), (1), (1), (1), (1)
Host Rabbit
(108), (36), (2), (1)
Clonality
Polyclonal
Conjugate This CRADD antibody is un-conjugated
(9), (9), (7), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(132), (72), (27), (16), (12), (9), (9), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-CRADD Antibody

Target Details CRADD Application Details Handling References for anti-CRADD Antibody (ABIN500561) Images
Specificity This antibody detects RAIDD at Center.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Peptide Affinity Chromatography
Immunogen RAIDD antibody was raised against a peptide corresponding to amino acids 99 to 117 of human RAIDD.
Isotype IgG

Target Details CRADD

Product Details anti-CRADD Antibody Application Details Handling References for anti-CRADD Antibody (ABIN500561) Images back to top
Antigen
Alternative Name RAIDD (CRADD Antibody Abstract)
Background Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1,RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals1,3. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.Synonyms: CRADD, Caspase and RIP adapter with death domain, Death domain-containing protein CRADD, RIP-associated protein with a death domain
Gene ID 8738
UniProt P78560
Research Area Cancer, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-CRADD Antibody Target Details CRADD Handling References for anti-CRADD Antibody (ABIN500561) Images back to top
Application Notes ELISA. Western blot: 1: 500 - 1: 1000, a 22 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CRADD Antibody Target Details CRADD Application Details References for anti-CRADD Antibody (ABIN500561) Images back to top
Buffer PBS containing 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CRADD Antibody (ABIN500561)

Product Details anti-CRADD Antibody Target Details CRADD Application Details Handling Images back to top
Background publications

Hofmann, Bucher, Tschopp: "The CARD domain: a new apoptotic signalling motif." in: Trends in biochemical sciences, Vol. 22, Issue 5, pp. 155-6, 1997

Duan, Dixit: "RAIDD is a new 'death' adaptor molecule." in: Nature, Vol. 385, Issue 6611, pp. 86-9, 1997

Ahmad, Srinivasula, Wang, Talanian, Litwack, Fernandes-Alnemri, Alnemri: "CRADD, a novel human apoptotic adaptor molecule for caspase-2, and FasL/tumor necrosis factor receptor-interacting protein RIP." in: Cancer research, Vol. 57, Issue 4, pp. 615-9, 1997

Images

Product Details anti-CRADD Antibody Target Details CRADD Application Details Handling References for anti-CRADD Antibody (ABIN500561) back to top
Supplier Images
Immunofluorescence (IF) image for anti-CRADD antibody (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain) (AA 99-117) (ABIN500561) Immunocytochemistry of RAIDD in HeLa cells with RAIDD antibody at 5 μg/ml.
Immunofluorescence (IF) image for anti-CRADD antibody (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain) (AA 99-117) (ABIN500561) Immunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 µg/ml.
Western Blotting (WB) image for anti-CRADD antibody (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain) (AA 99-117) (ABIN500561) Western blot analysis of RAIDD in whole cell lysates from HeLa (H) or K562 (K) cells ...