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anti-Cow (Bovine) CYLD antibody for Immunocytochemistry

Recommended CYLD Antibody (supplied by: Log in to see )

Antigen
Cylindromatosis (Turban Tumor Syndrome) (CYLD) Antibodies
  • 2010013M14Rik
  • 2900009M21Rik
  • BRSS
  • C130039D01Rik
  • CDMT
  • cyld
  • CYLD1
  • CYLDI
  • EAC
  • LRRGT00003
  • MFT
  • MFT1
  • mKIAA0849
  • Rp1
  • Rp1h
  • SBS
  • TEM
  • USPL2
Epitope
AA 500-600
15
13
10
6
5
3
2
2
1
1
1
1
1
Reactivity
Cow (Bovine)
51
44
28
4
2
2
1
1
1
1
1
Host
Rabbit
41
18
9
Clonality
Polyclonal
Conjugate
This CYLD antibody is un-conjugated
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
46
23
14
12
11
10
8
1
1
1
1
Supplier
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Catalog No. ABIN410066
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010194078 ABIN4301703 ICC IF WB DyLight 405 Rabbit AA 500-600, Internal Region Log in to see Polyclonal

Similar anti-CYLD Antibodies

Application / Reactivity Cow (Bovine)
ELISA 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Western Blotting (WB) 5 Antibodies

General

Antigen Cylindromatosis (Turban Tumor Syndrome) (CYLD) Antibodies
Epitope AA 500-600
(15), (13), (10), (6), (5), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(51), (44), (28), (4), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(41), (18), (9)
Clonality
Polyclonal
Conjugate This CYLD antibody is un-conjugated
(3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(46), (23), (14), (12), (11), (10), (8), (1), (1), (1), (1)
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Product Details anti-CYLD Antibody

Target Details CYLD Application Details Handling Images
No Cross-Reactivity Rat (Rattus)
Purification affinity purified
Immunogen Synthetic peptide made to an internal portion of human CYLD (within residues 500-600).

Target Details CYLD

Product Details anti-CYLD Antibody Application Details Handling Images back to top
Antigen
Alternative Name CYLD (CYLD Antibody Abstract)
Background Gene Symbol: CYLD
Gene ID 1540
UniProt Q9NQC7
Research Area Proteolysis / Ubiquitin
Pathways Apoptosis

Application Details

Product Details anti-CYLD Antibody Target Details CYLD Handling Images back to top
Application Notes This CYLD antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a doublet is seen ~120 kDa (Hellerbrand, et al.)
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:500 - 1:2000, Western Blot 2 µg/mL
Protocol Protocol specific for CYLD Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-CYLD primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.Immunocytochemistry/Immunofluorescence protocol for CYLD Antibody Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only

Handling

Product Details anti-CYLD Antibody Target Details CYLD Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C