anti-Dog (Canine) Cytochrome C antibody for Cytometry by Time of Flight

Recommended Cytochrome C Antibody (supplied by: Log in to see )

Antigen
Cytochrome C, Somatic (CYCS) Antibodies
  • cyct
  • cyc
  • hcs
  • CYCS
  • cycs
  • CYC
  • CG13263
  • Cyt C
  • Cyt c
  • Cyt-c1
  • CytC-1
  • CytC1
  • Cytc
  • Cytc-d
  • DC3
  • Dmel\\CG13263
  • bln
  • bs30f07.y1
  • cyt c
  • cyt c-d
  • cyt-c-d
  • cyt.c
  • cytochrome c
  • dc3
  • ms(2)04445
  • pDMc02
  • CG17903
  • Cyt-c2
  • CytC-2
  • Cytc-p
  • DC4
  • DMc01
  • Dmel\\CG17903
  • cyt c-p
  • cyt-c-p
  • cyt-c2
  • dc4
  • pDMc01
  • HCS
  • THC4
  • cytc
  • CYCSA
  • wu:fk52b01
  • zgc:86706
  • ENSMUSG00000058927
  • cytochrome c, somatic
  • cytochrome c, testis
  • Cytochrome c distal
  • Cytochrome c proximal
  • cytochrome c-like
  • cytochrome c, somatic b
  • predicted gene 10053
  • cycs
  • CYCS
  • cyct
  • Cyt-c-d
  • Cyt-c-p
  • LOC100724240
  • CYC
  • Cycs
  • cycsb
  • Gm10053
Epitope
AA 93-104
27
18
15
12
8
6
6
6
5
5
4
3
2
2
1
1
1
1
1
1
Reactivity
Amphibian, Bird (Avian), Dog (Canine), Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
351
292
220
102
75
63
37
34
28
28
28
27
21
11
10
9
8
7
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
260
151
6
2
Clonality
Monoclonal
Conjugate
This Cytochrome C antibody is un-conjugated
22
15
11
8
8
6
6
5
5
5
5
5
5
5
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
302
157
150
83
77
64
50
35
26
20
13
6
5
5
4
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN4301987
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4301988 CyTOF FACS IF IHC (p) WB Mouse IgG2b kappa AA 93-104 Log in to see SPM389

General

Antigen Cytochrome C, Somatic (CYCS) Antibodies
Epitope AA 93-104
(27), (18), (15), (12), (8), (6), (6), (6), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Amphibian, Bird (Avian), Dog (Canine), Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
(351), (292), (220), (102), (75), (63), (37), (34), (28), (28), (28), (27), (21), (11), (10), (9), (8), (7), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(260), (151), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytochrome C antibody is un-conjugated
(22), (15), (11), (8), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(302), (157), (150), (83), (77), (64), (50), (35), (26), (20), (13), (6), (5), (5), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Cytochrome C Antibody

Target Details Cytochrome C Application Details Handling Images
Specificity It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Purification Protein A or G purified
Immunogen Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
Clone SPM389
Isotype IgG2b kappa

Target Details Cytochrome C

Product Details anti-Cytochrome C Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytochrome C (CYCS Antibody Abstract)
Background Gene Symbol: CYCS
Molecular Weight Theoretical MW: 15 kDa
Gene ID 54205
Research Area Apoptosis/Necrosis, Metabolism
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top
Application Notes Western Blot : 0.5 - 1.0 μg/mL, Flow Cytometry : 0.5 - 1 μg/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 μg/mL, Immunofluorescence : 0.5 - 1.0 μg/mL, CyTOF-readyHu-chromosome location: 7p15.3 Immunohistochemistry-Paraffin 0.25 - 0.5 μg/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: No Preservative
Preservative Without preservative
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.