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anti-Fruit Fly (Drosophila melanogaster) Cytochrome C antibody for Flow Cytometry

Recommended Cytochrome C Antibody (supplied by: Log in to see )

Antigen
Cytochrome C, Somatic (CYCS) Antibodies
  • bln
  • bs30f07.y1
  • CG13263
  • CG17903
  • CYC
  • cyc
  • CYCS
  • cycs
  • CYCSA
  • cyct
  • cyt-c-d
  • cyt-c-p
  • Cyt-c1
  • cyt-c2
  • Cyt-c2
  • cyt.c
  • Cyt C
  • Cyt c
  • cyt c
  • Cytc
  • cytc
  • CytC-1
  • CytC-2
  • Cytc-d
  • cyt c-d
  • Cytc-p
  • cyt c-p
  • CytC1
  • cytochrome c
  • DC3
  • dc3
  • dc4
  • DC4
  • DMc01
  • Dmel\\CG13263
  • Dmel\\CG17903
  • ENSMUSG00000058927
  • hcs
  • HCS
  • ms(2)04445
  • pDMc01
  • pDMc02
  • THC4
  • wu:fk52b01
  • zgc:86706
Epitope
AA 93-104
26
18
13
11
8
6
6
6
4
4
4
3
2
1
1
1
1
1
1
1
Reactivity
Amphibian, Bird (Avian), Dog (Canine), Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
306
229
172
93
61
55
28
27
27
25
24
19
16
8
7
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
236
138
7
2
Clonality
Monoclonal
Conjugate
This Cytochrome C antibody is un-conjugated
20
14
10
7
7
6
6
5
5
5
5
5
5
5
5
5
5
5
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
277
156
137
81
68
67
48
34
19
11
11
8
6
6
5
4
4
4
3
3
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4301973
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010485934 ABIN4301975 FACS IF IHC (p) WB Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4301974 FACS IF IHC (p) WB Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN1724341 FACS ICC IHC (p) WB Mouse IgG2a Log in to see 7H8-2C12
0.0010485934 ABIN4301945 FACS ICC IF IHC IHC (p) WB Mouse IgG2b kappa Log in to see 7H8-2C12
0.0010485934 ABIN4302009 FACS IHC (p) WB PerCP Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302010 FACS IHC (p) WB PE Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302011 FACS IHC (p) WB DyLight 550 Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302012 FACS IHC (p) WB DyLight 350 Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302013 FACS IHC (p) WB DyLight 405 Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302014 FACS IHC (p) WB DyLight 405LS Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4301987 FACS IF IHC (p) WB Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4301997 FACS IHC (p) WB Alexa Fluor 405 Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4301998 FACS IHC (p) WB Alexa Fluor 488 Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4301999 FACS IHC (p) WB Alexa Fluor 647 Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302000 FACS IHC (p) WB Alexa Fluor 700 Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302001 FACS IHC (p) WB Allophycocyanin Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302002 FACS IHC (p) WB Biotin Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302003 FACS IHC (p) WB DyLight 650 Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302004 FACS IHC (p) WB FITC Mouse IgG2b kappa AA 93-104 Log in to see SPM389
0.0010485934 ABIN4302005 FACS IHC (p) WB DyLight 680 Mouse IgG2b kappa AA 93-104 Log in to see SPM389

Similar anti-Cytochrome C Antibodies

Application / Reactivity Dog (Canine) Horse (Equine) Human Mouse (Murine) Rat (Rattus) Fruit Fly (Drosophila melanogaster)
Western Blotting (WB) 55 Antibodies 80 Antibodies 220 Antibodies 117 Antibodies 169 Antibodies 53 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 8 Antibodies 7 Antibodies 7 Antibodies
Intracellular Staining (ICS) 4 Antibodies 2 Antibodies 2 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 30 Antibodies 16 Antibodies 23 Antibodies 5 Antibodies

General

Antigen Cytochrome C, Somatic (CYCS) Antibodies
Epitope AA 93-104
(26), (18), (13), (11), (8), (6), (6), (6), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Amphibian, Bird (Avian), Dog (Canine), Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
(306), (229), (172), (93), (61), (55), (28), (27), (27), (25), (24), (19), (16), (8), (7), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(236), (138), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytochrome C antibody is un-conjugated
(20), (14), (10), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(277), (156), (137), (81), (68), (67), (48), (34), (19), (11), (11), (8), (6), (6), (5), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Cytochrome C Antibody

Target Details Cytochrome C Application Details Handling Images
Specificity It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Purification Protein A or G purified
Immunogen Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
Clone SPM389
Isotype IgG2b kappa

Target Details Cytochrome C

Product Details anti-Cytochrome C Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytochrome C (CYCS Antibody Abstract)
Background Gene Symbol: CYCS
Molecular Weight Theoretical MW: 15 kDa
UniProt P99999
Research Area Metabolism, Apoptosis/Necrosis, Cancer
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top
Application Notes Western Blot 0.5 - 1.0 μg/mL, Flow Cytometry 0.5 - 1 μg/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 μg/mL, Immunofluorescence 0.5 - 1.0 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer Prepared in 1 mM PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.