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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||121 Antibodies||68 Antibodies||74 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Neutralization (Neut)||3 Antibodies||2 Antibodies||2 Antibodies|
|Intracellular Staining (ICS)||4 Antibodies||2 Antibodies||2 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies||10 Antibodies||17 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||35 Antibodies||23 Antibodies||26 Antibodies|
|Immunohistochemistry (IHC)||51 Antibodies||29 Antibodies||30 Antibodies|
|Antigen||Cytochrome C, Somatic (CYCS) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cytochrome C antibody is un-conjugated Alternatives|
|10 references available|
|Supplier||Log in to see|
Product Details anti-Cytochrome C AntibodyTarget Details Cytochrome C Application Details Handling References for anti-Cytochrome C Antibody (ABIN2666071) Images
|Purification||The antibody was purified by affinity chromatography.|
Target Details Cytochrome CProduct Details anti-Cytochrome C Antibody Application Details Handling References for anti-Cytochrome C Antibody (ABIN2666071) Images back to top
|Alternative Name||Cytochrome C (CYCS Antibody Abstract)|
|Background||Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain, the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.|
|Research Area||Cancer, Apoptosis/Necrosis, Metabolism|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling References for anti-Cytochrome C Antibody (ABIN2666071) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details References for anti-Cytochrome C Antibody (ABIN2666071) Images back to top
|Buffer||phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide. Final antibody concentration is 0.5 mg/mL.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Upon receipt, store between 2°C and 8°C.|
References for anti-Cytochrome C Antibody (ABIN2666071)Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling Images back to top
McGuire, Barlan, Griffin et al.: "Adenovirus type 5 rupture of lysosomes leads to cathepsin B-dependent mitochondrial stress and production of reactive oxygen species." in: Journal of virology, Vol. 85, Issue 20, pp. 10806-13, 2011 (PubMed).
She, Zhang, Marchionini et al.: "Molecular characterization of skeletal muscle atrophy in the R6/2 mouse model of Huntington's disease." in: American journal of physiology. Endocrinology and metabolism, Vol. 301, Issue 1, pp. E49-61, 2011 (PubMed).
Semenkova, Dudich, Dudich et al.: "Alpha-fetoprotein positively regulates cytochrome c-mediated caspase activation and apoptosome complex formation." in: European journal of biochemistry / FEBS, Vol. 270, Issue 21, pp. 4388-99, 2003 (PubMed).
Ferguson, Marietta, Van Den Berg: "UV-induced apoptosis is mediated independent of caspase-9 in MCF-7 cells: a model for cytochrome c resistance." in: The Journal of biological chemistry, Vol. 278, Issue 46, pp. 45793-800, 2003 (PubMed).
Zhang, Gerstein: "The human genome has 49 cytochrome c pseudogenes, including a relic of a primordial gene that still functions in mouse." in: Gene, Vol. 312, pp. 61-72, 2003 (PubMed).
Chandra, Liu, Tang: "Early mitochondrial activation and cytochrome c up-regulation during apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50842-54, 2002 (PubMed).
Shih, Wu, Hung et al.: "Abrin triggers cell death by inactivating a thiol-specific antioxidant protein." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21870-7, 2001 (PubMed).
Liu, Kim, Yang et al.: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, 1996 (PubMed).
Jemmerson, Johnson, Burrell et al.: "A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells." in: European journal of immunology, Vol. 21, Issue 1, pp. 143-51, 1991 (PubMed).
Goshorn, Retzel, Jemmerson: "Common structural features among monoclonal antibodies binding the same antigenic region of cytochrome c." in: The Journal of biological chemistry, Vol. 266, Issue 4, pp. 2134-42, 1991 (PubMed).
ImagesProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling References for anti-Cytochrome C Antibody (ABIN2666071) back to top