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anti-Pigeon Cytochrome C antibody for Flow Cytometry

Recommended Cytochrome C Antibody (supplied by: Log in to see )

Antigen
Cytochrome C, Somatic (CYCS) Antibodies
  • cyct
  • cyc
  • hcs
  • CYCS
  • cycs
  • CYC
  • CG13263
  • Cyt C
  • Cyt c
  • Cyt-c1
  • CytC-1
  • CytC1
  • Cytc
  • Cytc-d
  • DC3
  • Dmel\\CG13263
  • bln
  • bs30f07.y1
  • cyt c
  • cyt c-d
  • cyt-c-d
  • cyt.c
  • cytochrome c
  • dc3
  • ms(2)04445
  • pDMc02
  • CG17903
  • Cyt-c2
  • CytC-2
  • Cytc-p
  • DC4
  • DMc01
  • Dmel\\CG17903
  • cyt c-p
  • cyt-c-p
  • cyt-c2
  • dc4
  • pDMc01
  • HCS
  • THC4
  • cytc
  • CYCSA
  • wu:fk52b01
  • zgc:86706
  • ENSMUSG00000058927
  • cytochrome c, somatic
  • cytochrome c, testis
  • Cytochrome c distal
  • Cytochrome c proximal
  • cytochrome c-like
  • cytochrome c, somatic b
  • predicted gene 10053
  • cycs
  • CYCS
  • cyct
  • Cyt-c-d
  • Cyt-c-p
  • LOC100724240
  • CYC
  • Cycs
  • cycsb
  • Gm10053
Epitope
AA 1-80
28
16
15
11
8
6
6
5
5
4
3
3
2
2
1
1
1
1
1
1
Reactivity
Dog (Canine), Frog, Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Pigeon, Rat (Rattus)
345
285
215
99
76
62
37
33
28
28
25
22
21
10
9
8
7
7
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
251
141
7
2
Clonality
Monoclonal
Conjugate
This Cytochrome C antibody is un-conjugated
22
15
11
8
8
6
6
5
5
5
5
5
5
5
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
284
150
138
81
68
63
48
32
26
20
13
6
6
5
5
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN3025122
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3024593 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 66-104, AA 93-104 Log in to see SPM389
1 ABIN3024590 FACS IF IHC (p) WB Mouse IgG2b, kappa Log in to see CTC05
1 ABIN1724341 FACS ICC IHC (p) WB Mouse IgG2a Log in to see 7H8-2C12
1 ABIN3025124 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 1-80 Log in to see 7H8-2C12
1 ABIN3024594 FACS IF IHC (p) WB Mouse IgG2b, kappa AA 66-104, AA 93-104 Log in to see SPM389
1 ABIN3024592 FACS IF IHC (p) WB Mouse IgG2b, kappa Log in to see CTC05

Similar anti-Cytochrome C Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Fruit Fly (Drosophila melanogaster) Horse (Equine) Pigeon Frog
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies 2 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies 2 Antibodies 2 Antibodies 6 Antibodies 2 Antibodies 6 Antibodies
Western Blotting (WB) 19 Antibodies 59 Antibodies 81 Antibodies 234 Antibodies 147 Antibodies 194 Antibodies 53 Antibodies 8 Antibodies
Radioimmunoassay (RIA) 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Cytochrome C, Somatic (CYCS) Antibodies
Epitope AA 1-80
(28), (16), (15), (11), (8), (6), (6), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Frog, Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Pigeon, Rat (Rattus)
(345), (285), (215), (99), (76), (62), (37), (33), (28), (28), (25), (22), (21), (10), (9), (8), (7), (7), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(251), (141), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytochrome C antibody is un-conjugated
(22), (15), (11), (8), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(284), (150), (138), (81), (68), (63), (48), (32), (26), (20), (13), (6), (6), (5), (5), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Cytochrome C Antibody

Target Details Cytochrome C Application Details Handling Images
Characteristics It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Purification Protein G affinity chromatography
Immunogen Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon Cytochrome C was used as the immunogen for the Cytochrome C antibody.
Clone 7H8-2C12
Isotype IgG2b, kappa

Target Details Cytochrome C

Product Details anti-Cytochrome C Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytochrome C (CYCS Antibody Abstract)
Background It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top
Application Notes Optimal dilution of the Cytochrome C antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the Cyt°Chrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Cytochrome C antibody (Cytochrome C, Somatic) (AA 1-80) (ABIN3025122) Formalin-fixed, paraffin-embedded human pancreas stained with Cytochrome C antibody (...
Immunohistochemistry (IHC) image for anti-Cytochrome C antibody (Cytochrome C, Somatic) (AA 1-80) (ABIN3025122) Formalin-fixed, paraffin-embedded human salivary tumor stained with Cytochrome C anti...