You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) Cytochrome C antibody for BioImaging

Recommended Cytochrome C Antibody (supplied by: Log in to see )

Antigen
Cytochrome C, Somatic (CYCS) Antibodies
  • bln
  • bs30f07.y1
  • CG13263
  • CG17903
  • CYC
  • cyc
  • CYCS
  • cycs
  • CYCSA
  • cyct
  • cyt-c-d
  • cyt-c-p
  • Cyt-c1
  • cyt-c2
  • Cyt-c2
  • cyt.c
  • Cyt C
  • Cyt c
  • cyt c
  • Cytc
  • cytc
  • CytC-1
  • CytC-2
  • Cytc-d
  • cyt c-d
  • Cytc-p
  • cyt c-p
  • CytC1
  • cytochrome c
  • DC3
  • dc3
  • dc4
  • DC4
  • DMc01
  • Dmel\\CG13263
  • Dmel\\CG17903
  • ENSMUSG00000058927
  • hcs
  • HCS
  • ms(2)04445
  • pDMc01
  • pDMc02
  • THC4
  • wu:fk52b01
  • zgc:86706
Reactivity
Human, Mouse (Murine), Rat (Rattus)
182
109
100
48
24
17
16
11
11
8
8
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
137
113
7
2
Clonality
Monoclonal
Conjugate
This Cytochrome C antibody is un-conjugated
16
9
4
2
2
2
1
1
1
1
1
1
1
1
Application
BioImaging (BI), Immunoprecipitation (IP), Western Blotting (WB)
179
67
61
48
44
40
34
17
14
11
11
6
6
4
4
4
3
3
3
2
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN2689486
Contact our Customer Service for availability and price in your country.

Top referenced anti-Cytochrome C antibody for BioImaging

Similar anti-Cytochrome C Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 1 Antibodies 1 Antibodies
ELISA 13 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 26 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 75 Antibodies

General

Antigen Cytochrome C, Somatic (CYCS) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(182), (109), (100), (48), (24), (17), (16), (11), (11), (8), (8), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(137), (113), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytochrome C antibody is un-conjugated
(16), (9), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application BioImaging (BI), Immunoprecipitation (IP), Western Blotting (WB)
(179), (67), (61), (48), (44), (40), (34), (17), (14), (11), (11), (6), (6), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-Cytochrome C Antibody

Target Details Cytochrome C Application Details Handling References for anti-Cytochrome C Antibody (ABIN2689486) Images
Brand BD Pharmingen™
Characteristics A cytochrome is an electron-transporting protein that contains a heme prosthetic group. Cytochromes have been known to be essential components of the mitochondrial respiratory chain since 1925. The iron atom of the heme group in cytochromes alternates between a reduced ferrous (+2) state and an oxidized ferric (+3) state during electron transport in oxidative phosphorylation. Cytochromes are classified into four groups (a, b, c and d) according to spectrochemical characteristics, and there are five cytochromes between coenzyme QH2 and O2 in the electron transport chain. Cytochrome c is a water-soluble protein that either promotes cell survival or death, depending upon its intracellular location. In healthy cells, it is a peripheral membrane protein of the mitochondria that transports electrons from the coenzyme QH2 cytochrome c reductase complex to the cytochrome c oxidase complex. When proapoptotic stimuli induce breakdown of the mitochondria, cytochrome c is released to the cytosol where it functions in the activation of caspases that trigger apoptosis. The 6H2.B4 monoclonal antibody has been reported to recognize the native and not the denatured form of rat, mouse, and human cytochrome c. Furthermore, studies utilizing competitive ELISA indicate that mAb 6H2.B4 binds to a region around residue 62 of rat cytochrome c. The 6H2.B4 monoclonal antibody is not useful for western blot analysis. For the western blot application, clone 7H8.2C12 (cat.no. 556433) would be recommended. Suggested positive controls for detecting cytochrome c include P388D mouse lymphoma cells (ATCC CCL-46), HeLa human carcinoma cells (ATCC CCL-2), Jurkat T leukemia cells (ATCC TIB-152) and NIH 3T3 mouse fibroblast cells (ATCC CRL-1658). This antibody is routinely tested by immunoprecipitation. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Cytochrome c - Purified - Clone 6H2.B4 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms, Rat - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat Cytochrome c
Clone 6H2-B4
Isotype IgG1, kappa

Target Details Cytochrome C

Product Details anti-Cytochrome C Antibody Application Details Handling References for anti-Cytochrome C Antibody (ABIN2689486) Images back to top
Antigen
Alternative Name Cytochrome C (CYCS Antibody Abstract)
Molecular Weight 15 kDa
Research Area Apoptosis/Necrosis, Metabolism
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling References for anti-Cytochrome C Antibody (ABIN2689486) Images back to top
Application Notes Confirmed by immunoprecipitation: Human Confirmed during development by immunoprecipitation: Mouse, Rat
Restrictions For Research Use only

Handling

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details References for anti-Cytochrome C Antibody (ABIN2689486) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-Cytochrome C Antibody (ABIN2689486)

Product Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling Images back to top
Product cited in:

Garrido, Galluzzi, Brunet, Puig, Didelot, Kroemer: "Mechanisms of cytochrome c release from mitochondria." in: Cell death and differentiation, Vol. 13, Issue 9, pp. 1423-33, 2006

Rossé, Olivier, Monney, Rager, Conus, Fellay, Jansen, Borner: "Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c." in: Nature, Vol. 391, Issue 6666, pp. 496-9, 1998

Liu, Kim, Yang, Jemmerson, Wang: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, 1996

Jemmerson, Johnson: "Different functional boundaries for the major antigenic region of two cytochromes c." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4428-32, 1991