anti-Human DFFA antibody for Enzyme Immunoassay

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • DFFA
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • DNA fragmentation factor, alpha subunit
  • DFFA
  • dffa
  • Dffa
Epitope
C-Term, AA 302-331
28
26
19
16
15
14
12
8
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
167
85
41
2
2
1
1
1
Host
Rabbit
193
35
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
192
71
48
37
34
28
19
13
5
4
4
3
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN951895
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN272297 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN5554751 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5554752 EIA WB Rabbit IgG N-Term Log in to see Polyclonal

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope C-Term, AA 302-331
(28), (26), (19), (16), (15), (14), (12), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(167), (85), (41), (2), (2), (1), (1), (1)
Host Rabbit
(193), (35), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(192), (71), (48), (37), (34), (28), (19), (13), (5), (4), (4), (3), (2), (2), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling References for anti-DFFA antibody (ABIN951895) Images
Specificity This antibody recognizes Human DFFA / ICAD (C-term).
Purification Affinity Chromatography on Protein A
Immunogen KLH conjugated synthetic peptide between 302~331 amino acids from the C-terminal region of Human DFFA / ICAD. Genename: DFFA
Isotype Ig

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling References for anti-DFFA antibody (ABIN951895) Images back to top
Antigen
Alternative Name DFFA / ICAD (DFFA Antibody Abstract)
Background DFFA is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
Molecular Weight 36522 Da
Gene ID 1676
NCBI Accession NP_004392
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling References for anti-DFFA antibody (ABIN951895) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details References for anti-DFFA antibody (ABIN951895) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-DFFA antibody (ABIN951895)

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling Images back to top
Background publications

Ninios, Sekeri-Pataryas, Sourlingas: "Histone H1 subtype preferences of DFF40 and possible nuclear localization of DFF40/45 in normal and trichostatin A-treated NB4 leukemic cells." in: Apoptosis : an international journal on programmed cell death, Vol. 15, Issue 2, pp. 128-38, 2010

Banas, Skotniczny, Basta: "DFF45 expression in ovarian endometriomas." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 146, Issue 1, pp. 87-91, 2009

Trynka, Zhernakova, Romanos, Franke, Hunt, Turner, Bruinenberg, Heap, Platteel, Ryan, de Kovel, Holmes, Howdle, Walters, Sanders, Mulder, Mearin, Verbeek, Trimble, Stevens, Kelleher, Barisani et al.: "Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling. ..." in: Gut, Vol. 58, Issue 8, pp. 1078-83, 2009

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling References for anti-DFFA antibody (ABIN951895) back to top
Supplier Images
Western Blotting (WB) image for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 302-331), (C-Term) antibody (ABIN951895) Western blot analysis of DFFA (arrow) using DFFA / ICAD Antibody (C-term) Cat.-No AP5...
Western Blotting (WB) image for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 302-331), (C-Term) antibody (ABIN951895) Western blot analysis of DFFA / ICAD Antibody (C-term) in K562 cell line lysates (35u...