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anti-Human DFFA antibody for Immunocytochemistry

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • DFFA
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • DNA fragmentation factor, alpha subunit
  • DFFA
  • dffa
  • Dffa
Epitope
C-Term
28
26
19
16
15
15
12
8
5
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
162
82
40
2
2
1
1
1
Host
Rabbit
185
36
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
187
67
44
33
31
27
16
13
6
4
4
3
2
2
1
Supplier
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Catalog No. ABIN4305087
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN214483 ICC IHC (p) IP WB Rabbit AA 313-331 Log in to see Polyclonal
1 ABIN4305083 ICC IF WB Mouse IgG1 Transcript Variant 1 Log in to see 5C4
1 ABIN2119453 ICC IHC IP WB Rabbit IgG AA 51-305 Log in to see Polyclonal
1 ABIN1733624 ICC IHC (p) IP WB Rabbit IgG AA 313-331 Log in to see Polyclonal
1 ABIN4305088 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN4305089 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN1820461 ICC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN4305082 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1030360 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2598329 ICC IF IHC (p) IP WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN1821637 ICC WB Rabbit Log in to see Polyclonal
1 ABIN1886868 ICC ELISA WB Rabbit AA 313-331 Log in to see Polyclonal
1 ABIN1582777 ICC ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-DFFA antibody for Immunocytochemistry

Similar anti-DFFA Antibodies

Application / Reactivity Human
Dot Blot (DB) 4 Antibodies
ELISA 57 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 142 Antibodies

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope C-Term
(28), (26), (19), (16), (15), (15), (12), (8), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(162), (82), (40), (2), (2), (1), (1), (1)
Host Rabbit
(185), (36), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(187), (67), (44), (33), (31), (27), (16), (13), (6), (4), (4), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling References for anti-DFFA Antibody (ABIN4305087) Images
Purification Ion exchange chromatography
Immunogen Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45. The immunogen is located within the last 50 amino acids of DFF45.

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling References for anti-DFFA Antibody (ABIN4305087) Images back to top
Antigen
Alternative Name DFF45/ICAD (DFFA Antibody Abstract)
Background Gene Symbol: DFFA
Molecular Weight Theoretical MW: 45 kDa
Gene ID 1676
NCBI Accession NP_004392
Research Area DNA/RNA, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling References for anti-DFFA Antibody (ABIN4305087) Images back to top
Application Notes Western Blot 0.5 - 1 μg/mL, ELISA 1:100-1:2000, Immunohistochemistry 5 μg/mL, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5 μg/mLDFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blotat 1:1000 to 1:2000 dilution and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details References for anti-DFFA Antibody (ABIN4305087) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-DFFA Antibody (ABIN4305087)

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling Images back to top
Product cited in:

Nagata, Kishi, Liu, Matsuda, Imanaka, Tsukada, Kang, Muraguchi: "The regulation of DNAse activities in subcellular compartments of activated thymocytes." in: Immunology, Vol. 105, Issue 4, pp. 399-406, 2002

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling References for anti-DFFA Antibody (ABIN4305087) back to top
Supplier Images
Western Blotting (WB) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (C-Term) (ABIN4305087) Western Blot: DFF45/ICAD Antibody [NBP1-77034] - Analysis of HeLa (H), Jurkat (J), A4...
Western Blotting (WB) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (C-Term) (ABIN4305087) Western Blot: DFF45/ICAD Antibody [NBP1-77034] - Western blot analysis of 50 ug of to...
Immunofluorescence (IF) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (C-Term) (ABIN4305087) Immunocytochemistry/Immunofluorescence: DFF45/ICAD Antibody [NBP1-77034] - Staining o...