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anti-Human DFFA antibody for Immunocytochemistry

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • A330085O09Rik
  • DFF-45
  • DFF1
  • DFF35
  • Dff45
  • DFFA
  • ICAD
  • ICAD-L
  • ICAD-S
Epitope
AA 313-331
38
29
18
16
13
13
11
8
5
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
165
78
32
1
1
Host
Rabbit
194
33
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
187
76
48
32
31
20
17
11
6
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN2156251
€ 510.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.001009452 ABIN214483 ICC IHC (p) IP WB Rabbit AA 313-331 Log in to see Polyclonal
0.001009452 ABIN1733624 ICC IHC (p) IP WB Rabbit IgG AA 313-331 Log in to see Polyclonal
0.001009452 ABIN2119453 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 51-305 Log in to see Polyclonal
0.001009452 ABIN1820461 ICC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.001009452 ABIN1821637 ICC WB Rabbit Log in to see Polyclonal
0.001009452 ABIN1030360 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.001009452 ABIN1886868 ICC ELISA WB Rabbit AA 313-331 Log in to see Polyclonal
0.001009452 ABIN2598329 ICC Rabbit Internal Region Log in to see Polyclonal
0.001009452 ABIN4305083 ICC IF WB Mouse IgG1 Transcript Variant 1 Log in to see 5C4
0.001009452 ABIN1582777 ICC ELISA WB Rabbit Log in to see Polyclonal
0.001009452 ABIN4305082 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.001009452 ABIN4305087 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.001009452 ABIN4305088 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.001009452 ABIN4305089 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.001009452 ABIN3049155 ICC IF IHC WB Rabbit Log in to see Polyclonal

Similar anti-DFFA Antibodies

Application / Reactivity Human
Dot Blot (DB) 4 Antibodies
ELISA 64 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 20 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 139 Antibodies

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope AA 313-331
(38), (29), (18), (16), (13), (13), (11), (8), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(165), (78), (32), (1), (1)
Host Rabbit
(194), (33), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
(187), (76), (48), (32), (31), (20), (17), (11), (6), (4), (4), (3), (2), (1), (1), (1)
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling Images
Predicted Reactivity Human
Characteristics Apoptosis Is Related To Many Diseases And Induced By A Family Of Cell Death Receptors And Their Ligands. Cell Death Signals Are Transduced By Death Domain Containing Adapter Molecules And Members Of The Caspase Family Of Proteases. These Death Signals Finally Cause The Degradation Of Chromosomal Dna By Activated Dnase. A Human 45 Kda Dna Fragmentation Factor (DFF45) Was Identified Recently Which Was Cleaved By Caspase-3 During Apoptosis. Mouse Homologue Of Human Dff45 Was Identified As A Dnase Inhibitor Designated Icad. Upon Cleavage Of Dff45/Icad, A Caspase Activated Deoxyribonuclease (DFF40/CAD) Is Released And Activated And Eventually Causes The Degradation Of Dna In The Nuclei. Therefore, The Cleavage Of Dff45/Icad, Which Causes Dff40/Cad Activation And Dna Degradation, Is The Hallmark Of Apoptotic Cell Death.
Purification Antibody is DEAE purified
Immunogen The immunogen for anti-DFF45 antibody: antibody was raised against a peptide corresponding to AA 313-331 of human DFF45.

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name DFF45 (DFFA Antibody Abstract)
Background (CT) Dna Fragmentation Factor & Inhibitor Of Cad
Gene ID 1676
NCBI Accession NP_004392
UniProt O00273
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling Images back to top
Application Notes DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blotat 1:1000 to 1:2000 dilution and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells
Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details Images back to top
Handling Advice As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
Storage 4 °C
Storage Comment DFF45 antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Expiry Date 12 months

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 313-331) (ABIN2156251) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 313-331) antibody
Immunocytochemistry (ICC) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (AA 313-331) (ABIN2156251) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 313-331) antibody (Image 2)